Satellite Banner
Molecular & Clinical Diagnostics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

University of Toronto Breakthrough Allows Fast, Reliable Identification of Pathogens

Published: Friday, June 21, 2013
Last Updated: Friday, June 21, 2013
Bookmark and Share
Researchers have created an electronic chip that can analyze blood and other clinical samples for infectious bacteria with record-breaking speed.

Life-threatening bacterial infections cause tens of thousands of deaths every year in North America but current methods of culturing bacteria in the lab can take days to report the specific source of the infection, and even longer to pinpoint the right antibiotic that will clear the infection.

The new technology, reported in the journal Nature Communications, can identify the pathogen in a matter of minutes, and looks for many different bacteria and drug resistance markers in parallel, allowing rapid and specific identification of infectious agents.

“Overuse of antibiotics is driving the continued emergence of drug-resistant bacteria,” said Shana Kelley (Pharmacy and Biochemistry), a senior author of the study. “A chief reason for use of ineffective or inappropriate antibiotics is the lack of a technology that rapidly offers physicians detailed information about the specific cause of the infection.”

Many infections are resistant to first-line antibiotics and there is an urgent, unmet need for technologies that can allow bacterial infections to be rapidly and specifically diagnosed, Kelley said.
The researchers developed an integrated circuit that could detect bacteria at concentrations found in patients presenting with a urinary tract infection.

“The chip reported accurately on the type of bacteria in a sample, along with whether the pathogen possessed drug resistance,” explained Chemistry Ph.D. student Brian Lam, the first author of the study.

One key to the advance was the design of an integrated circuit that could accommodate a panel of many biomarkers.

“The team discovered how to use the liquids in which biological samples are immersed as a ‘switch’ – allowing us to look separately for each biomarker in the sample in turn,” said Ted Sargent (Electrical and Computer Engineering), the other senior author of the report.

“The solution-based circuit chip rapidly and identifies and determines the antibiotic resistance of multiple pathogens – this represents a significant advance in biomolecular sensing,” said Paul S. Weiss, Kavli Chair in NanoSystems Science and Director of the California NanoSystems Institute at UCLA.

Ihor Boszko, director of business development at Xagenic, a Toronto-based in vitro diagnostics company said the breakthrough could have significant practical implications.

"This kind of highly sensitive, enzyme-free electrochemical detection technology will have tremendous utility for near patient clinical diagnostic," said Boszko. "Multiplexing of in vitro diagnostic approach adds the capability of simultaneously testing for multiple viruses or bacteria that produce similar clinical symptoms.

"It also allows for simple and cost effective manufacturing of highly multiplexed electrochemical detectors, which will certainly have a significant impact on the availability of effective diagnostic tools."


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,400+ scientific posters on ePosters
  • More than 4,900+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

New Chip Makes Testing For Antibiotic-Resistant Bacteria Faster, Easier
Researchers at the University of Toronto design diagnostic chip to reduce testing time from days to one hour, allowing doctors to pick the right antibiotic the first time.
Wednesday, May 27, 2015
Scientific News
AACC 2016 Sees Clinical Chemistry Labs Drive Precision Medicine Offerings
Biomarker assays to enable precision medicine and risk assessment, mass spec-based tests designed for use in clinical labs large and small, and liquid biopsy technology captured the spotlight at the AACC annual meeting.
Diagnosing Tumors of Unknown Origin
EPICUP® test is a tool that helps to identify up to 87% of cancers of unknown origin (COD).
New Possibilities Tumor Research
Grazer researchers say gene activity of the tumor from the analysis of circulating DNA in blood ahead.
Parkinson’s Test Could Aid Early Detection
A test that can detect Parkinson’s disease in the early stages of the illness has moved a step closer.
Linkage Biosciences Awarded NHS Contract
Comapny announces that it has been awarded a four-year contract by NHS Blood and Transplant (NHSBT) in the UK for implementation of the LinkSeq™ Real-Time PCR HLA typing product.
Diagnosing Bacterial Infections in Blood Samples
Researchers have diagnosed a bacterial infection from a blood sample in infants.
Neurodvelopmental Disorder Cause Linked to SON Gene
A genetic link has been discovered for a previously unxplained neurodevelopmental disorder.
World's Most In-Depth Study to Detect Alzheimer's Disease
A multisite team will see the most thorough and vigorous testing for Alzheimer's ever performed on volunteers.
Personalised Medicine: Dose by Design
Personalised medicine holds the promise of a new approach to healthcare, tailored exactly to our individual needs, as Congenica's Nick Lench discussed on a recent BBC Radio 4 programme.
Misdiagnosis in HCM Tests
Genetic tests for potentially fatal heart anomaly can misdiagnose condition in black Americans.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!