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Thursday, October 23, 2014
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High Titre BacMam viruses improve transduction efficiency of mammalian cells
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King

We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.

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Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn

We report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, with a cage surface consisting of positively charged amino groups, which is expected to bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y) exhibits enhanced targeting of U-251 GBM cell lines.

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Next Generation Multivalent PRINT Nanoparticle Vaccine Targeting Pneumococcal Disease
Shyam Rele,1 RiLee Robeson,1 Anton Beletskii,1 Jeremy Hansen,1 Meredith Earl,1 Gabe Fawcett,1* Marquita Lilly,1 Joseph Marchand,1 Michele Stone,1 Camille Bernasconi,1 Jinny Conley,1 Michael Hunter,1 Ramya Yadavalli,1 Nicole Meyer,1 Lara Kelly,1 Ben Yerxa,1 Jeff Maisonneuve,2 Mark Alderson,2 Frank Malinoski1

A new generation PRINT nanoparticle Pneumococcal vaccine matrix has been designed to mimic size and structural aspects of bacterial pathogens to efficiently present and deliver polysaccharide and protein antigens to elicit robust immune responses.

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Low-cost Electrochemical Microfluidic Immunoarrays for Cancer Diagnostics
Brunah A. Otieno, Colleen E. Krause, Gregory W. Bishop, James F. Rusling

This poster describes the development and validation of a simple, low-cost, semi-automated microfluidic device for cancer biomarker protein detection to aid in cancer diagnosis and therapy monitoring.

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Evidence for Integrating Point of Care Diagnostics as an Integral Part of Primary Healthcare in South African Rural Communities: A People-centred Health System-strengthening Approach
T.P. Mashamba-Thompson, N. Moodley, M.M. Moshabela

Diagnostic systems designed in developed countries may not be immediately appropriate for use in the target developing countries. Therefore, a rigorous assessment for POC diagnostics outcomes of interest in the setting for which the test is designed should be carried out prior to adoption and scale up.

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Correlation between plasma levels of lutein and oxidized low density lipoproteins: a short human intervention study
Tiziana Bacchetti, Gianna Ferretti

The increase of plasma carotenoids, in particular lutein, after dietary treatment for two weeks is associated with a significant protective effect against lipid peroxidation of plasma lipoproteins.

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DETERMINATION OF HOMOCYSTEINE THIOLACTONE IN URINE BY FIELD AMPLIFIED SAMPLE INJECTION AND SWEEPING MEKC METHOD WITH UV DETECTION
Paulina Furmaniak, Pawel Kubalczyk, Kamila Borowczyk, Rafal Glowacki

* The procedure does not overload the capillary and has positive effect on the increase in peak hight,
* The method allows about 70-fold increase in method sensitivity,
* The limits of detection and quantification are 0.1 nmol and 0.09 nmol of Hcy-thiolactone in 1 mL of urine, respectively,
* Presented assay can be used for the determination of Hcy-thiolactone in biological samples.

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A Rapid and Effective Tool for Monitoring Monoclonal Antibody Production
B. Bhullar, Ph.D., Carlina Hui, B.Sc., M. Hariharan, M.Sc., J. Hewitt, B.Sc , and N. Vats, Ph.D.

Monoclonal antibodies (Mab) are used in a variety of fields from diagnostics to therapeutics. Manufacturers of in vitro diagnostics (IVD) commonly use third-party suppliers to meet their needs for specified monoclonal antibodies.

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A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Christian Le Cocq, Kyeong Soo Jeong, Arjun Vadapalli, Joseph Ong, Elin Agne, Filip Karlsson, Ashutosh Ashutosh, Francisco Useche, Jayati Ghosh, Henrik Johansson, Scott Happe, Douglas Roberts, and Holly Hogrefe

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

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A multianalyte algorithm PCR-based blood test outperforms single analyte ELISA-based blood tests for neuroendocrine tumor detection
Mark Kidd, Irvin M Modlin, Daniele Alaimo, Stephen Callahan, Nancy Teixiera, Lisa Bodei, Ignat Drozdov

In a prospective study, in age-/sex- and ethnicity-matched patients and controls (n=82), a 51 panel multigene blood transcript test (NETest) was identified to be significantly more sensitive and accurate (>93%) than any single analyte assay (Chromogranin A, Pancreastatin or Neurokinin A) for neuroendocrine tumor detection.

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Showing Results 1 - 10 of 56
Scientific News
CDC Develops a New, Faster Lab Test for Enterovirus D68
Confirmed cases will appear to rise as agency accelerates specimen testing.
New 'Lab-on-a-Chip' Could Revolutionize Early Diagnosis of Cancer
Faster result times, reduced costs, minimal sample demands and better sensitivity of analysis.
NanoString, Brigham and Women’s Hospital Collaborate
Research collaboration to accelerate translation of genomic discoveries into clinical diagnostics in oncology.
Biologists Find An Early Sign Of Cancer
Patients show boost in certain amino acids years before diagnosis of pancreatic cancer.
New DNA Sequencing Method to Diagnose Tuberculosis
Teams aim to test the metagenomics technique on a wide range of samples.
Inivata Raises £4m to Realize the Potential of ctDNA Analysis
New spin-out from Cancer Research UK launched with funding from Imperial Innovations, Cambridge Innovation Capital and Johnson & Johnson Development Corporation.
Blood Test May Help Determine Psychosis Risk
A study led by University of North Carolina at Chapel Hill researchers represents an important step forward in the accurate diagnosis of people who are experiencing the earliest stages of psychosis.
Asuragen’s Xpansion Interpreter® Test Study of 1,040 Transmissions of Fragile X Alleles
Study reveals personalized risks for expansion from parent to child.
Blood Test To Diagnose Depression
Test identifies nine blood markers tied to depression; predicts who will benefit from therapy.
Study Aims to Develop Breath Test for COPD
A new clinical iniative, lead by NYU Langone Medical Center, aims to determine a breath test’s effectiveness to identify volatile organic compounds (VOCs) in human breath that are biomarkers of chronic obstructive pulmonary disease (COPD).
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