|Recent Technical Developments in the Standardized Separation and Measurement of Extracellular Vesicles|
Amy Phillips (1), Robert Vogel(2), and Murray F. Broom(1)
We present robust and practical solutions for the standardization of EV isolation and concentration measurement. EV concentration also needs to have a defined size range. With these solutions, significant improvement in the reproducibility and comparability of results between different researchers is possible.
|Developing a digital histopathology platform to support an international diabetes biobank|
Sucaet Yves,. Smeets Silke, Waelput Wim, In’t Veld P.eter
An online histopathology platform combines tissue samples and online digital histopathology images. We combine detailed online patient data with whole slide images. This allows potential users of the tissue bank material to select the tissue and patient characteristics before entering into a collaborative agreement. The digital histopathology platform also allows different pathology laboratories in Belgium to discuss histopathological slides, and facilitates research collaboration.
|High Titre BacMam viruses improve transduction efficiency of mammalian cells|
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King
We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.
|Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection|
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn
We report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, with a cage surface consisting of positively charged amino groups, which is expected to bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y) exhibits enhanced targeting of U-251 GBM cell lines.
|Performance of a hybrid gamma-optical camera for improved utility in diagnostic imaging|
L.K. Jambi1, J.E. Lees1, S.L. Bugby1, B.S. Bhatia1,2, M.S. Alqahtani1, W.R. McKnight1, A.H. Ng3 and A.C. Perkins3
Performance of a hybrid gamma-optical camera for improved utility in diagnostic imaging
|Next Generation Multivalent PRINT Nanoparticle Vaccine Targeting Pneumococcal Disease|
Shyam Rele,1 RiLee Robeson,1 Anton Beletskii,1 Jeremy Hansen,1 Meredith Earl,1 Gabe Fawcett,1* Marquita Lilly,1 Joseph Marchand,1 Michele Stone,1 Camille Bernasconi,1 Jinny Conley,1 Michael Hunter,1 Ramya Yadavalli,1 Nicole Meyer,1 Lara Kelly,1 Ben Yerxa,1 Jeff Maisonneuve,2 Mark Alderson,2 Frank Malinoski1
A new generation PRINT nanoparticle Pneumococcal vaccine matrix has been designed to mimic size and structural aspects of bacterial pathogens to efficiently present and deliver polysaccharide and protein antigens to elicit robust immune responses.
|Low-cost Electrochemical Microfluidic Immunoarrays for Cancer Diagnostics|
Brunah A. Otieno, Colleen E. Krause, Gregory W. Bishop, James F. Rusling
This poster describes the development and validation of a simple, low-cost, semi-automated microfluidic device for cancer biomarker protein detection to aid in cancer diagnosis and therapy monitoring.
|Evidence for Integrating Point of Care Diagnostics as an Integral Part of Primary Healthcare in South African Rural Communities: A People-centred Health System-strengthening Approach|
T.P. Mashamba-Thompson, N. Moodley, M.M. Moshabela
Diagnostic systems designed in developed countries may not be immediately appropriate for use in the target developing countries. Therefore, a rigorous assessment for POC diagnostics outcomes of interest in the setting for which the test is designed should be carried out prior to adoption and scale up.
|Correlation between plasma levels of lutein and oxidized low density lipoproteins: a short human intervention study|
Tiziana Bacchetti, Gianna Ferretti
The increase of plasma carotenoids, in particular lutein, after dietary treatment for two weeks is associated with a significant protective effect against lipid peroxidation of plasma lipoproteins.