Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>Events>This Event
  Events - May 2013


3 days Hands-on qPCR

20 May 2013 - 22 May 2013 - TATAA Biocenter, Prague



Bookmark and Share


Tataa logga.png

3 DAYS HANDS-ON QPCR

Target audience: Beginners to Medium experienced qPCR users

Entrance qualifications: Basic Molecular biology or similar

Description: The basic real-time qPCR course. You will aquire a comprehensive overview of the possibilities with real-time PCR, how to use it and how to analyze the results. The course contains:

Day 1

Basic PCR theory
The theory of real-time PCR
Applications and possibilities of qPCR. Comparison of qPCR with regular PCR.
Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons, etc)
Different instrument platforms and their typical uses
Primer design
The problem of primer-dimer formation and how to minimize them
Probe design
Experimental design and optimization
Basic data handling and analysis

Day 2

Introduction to quantification principles
Quantification strategies, uses and limitations
Calculations using different relative quantification methods
Strategies for normalization of qPCR data
In situ calibration for compensation of inhibition in samples
Absolute quantification

Day 3

Sample Preparation (Extraction of RNA and DNA)
Principles of RT
Priming methods for RT
RT optimization
The MIQE guidelines
Group discussion, bring your own questions




Further information
Scientific News
Open Source Seed Initiative – A Welcome Boost to Global Crop Breeding
A team of plant breeders, farmers, non-profit agencies, seed advocates, and policymakers have created the Open Source Seed Initiative.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
A New Way Out for Stem Cells
Researchers at North Carolina State University have discovered that therapeutic stem cells exit the bloodstream in a different manner than was previously thought.
One Giant Leap for the Future of Safe Drug Delivery
Sheffield engineers make major breakthrough in developing silk ‘micro-rockets’ that can be used safely in biological environments.
Designing Potential AIDS Vaccine Candidates
Findings represent ‘big accomplishment’ in biomedical engineering and design.
Anticancer Drug Stops Ebola Virus Molecule in its Tracks
A team of scientists from the University of Oxford have successfully mapped the structure of the Ebola virus molecule that drives the attack strategy and leads to fatal infections in humans.
Assessing the Effectiveness of Genome-Editing Technologies
Researchers have developed a cost-effective and rapid method for assessing edits generated by CRISPR-Cas9 and other genome-editing technologies.
Anthrax Proteins Might Help Treat Cancerous Tumors
Studies in mice reveal novel treatment regimen.
New Cancer Drug Target Found in Dual-Function Protein
Findings from a study from TSRI have shown that targeting a protein called GlyRS might help to halt cancer growth.
Key to Chronic Fatigue Syndrome is in Your Gut, Not Head
Researchers report they have identified biological markers of the disease in gut bacteria and inflammatory microbial agents in the blood.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!