Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
Become a Member | Sign in
Home>News>This Article

A Simple HPLC Method for Determining the Purine Content of Beer and Beer-Like Alcoholic Beverages

Published: Wednesday, May 29, 2013
Last Updated: Wednesday, May 29, 2013
Bookmark and Share
In this article researchers from Teikyo University report an improved method for determining small amounts of purines in alcoholic beverages.

Several methods for quantifying the purine content in food and drink have been described using high-performance liquid chromatography (HPLC). We have developed an improved HPLC method that is based on a method reported by Kaneko et al. and that is more sensitive yet simple, and suitable for determining the purine content of beer and beer-like alcoholic beverages. Quantitative HPLC separation was performed on a Shodex Asahi Pak GS-320HQ column with an isocratic elution of 150 mmol/L sodium phosphate buffer (H3PO4/NaH2PO4 = 20:100 (v/v)). The retention times for the four analytes, namely, adenine, guanine, hypoxanthine and xanthine, were 19.9, 25.0, 29.3 and 43.0 min, respectively. The resolution was good, and there was no excessive interference from the other compounds in the beverages at these retention times. Furthermore, the detection limit for all the analytes was improved to less than 0.0075 mg/L, and all the calibration curves showed good linearity (r(2) > 0.999) between 0.013 and 10 mg/L for adenine and guanine, and between 0.025 and 10 mg/L for hypoxanthine and xanthine. The pretreatment was simplified by removing some procedures and optimizing the perchloric acid hydrolysis and the enzymatic peak-shift assay. We reduced the sample dilution rate by almost 50%, and the time spent on pretreatment from 4 days to only 180 min. The recovery of the analytes from spiked samples was 94.8 - 103.8%. This method may be useful for evaluating quantitative and qualitative differences in the purine content of beer and beer-like alcoholic beverages.

The article is published online in the journal Analytical Sciences and is free to access.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Acetaldehyde and Formaldehyde Content in Foods
Korean researchers have determined the content of the toxic and carcinogenic aldehydes, acetaldehyde and formaldehyde, in a variety of food groups.
Monday, November 09, 2015
Comparison of Caffeinated and Decaffeinated Coffee - Implications for Alzheimer's Disease
Researchers from the National University of Singapore successfully demonstrated the viability of GC-TOF-MS as an analytical platform for metabolomics analysis of coffee samples.
Monday, September 08, 2014
Immunochromatographic Assay for Rapid Salmonella Detection
The test strip described in the study would be beneficial for poultry farms and other veterinary production for the monitoring of S. Typhimurium and/or S. Enteritidis contamination.
Tuesday, August 12, 2014
Application of MALDI-TOF MS for the Identification of Food Borne Bacteria
MALDI-TOF MS is a promising platform for quick, flexible, and reliable identification of food microbial isolates.
Tuesday, April 29, 2014
Monitoring and Risk Assessment of Pesticide Residues in Commercially Dried Vegetables
The study aimed to analyze the presence of 253 pesticides commonly used on dried vegetables and to check their compliance with existing regulations.
Tuesday, March 11, 2014
Determination of Cyanogenic Compounds in Edible Plants by Ion Chromatography
Ion chromatography following acid hydrolysis was employed to prevent thiocyanate interference of the colorimetric methods.
Tuesday, December 10, 2013
LC-MS/MS-Based Multibiomarker Approaches for the Assessment of Human Exposure to Mycotoxins
To overcome the disadvantages of indirect methods, biomarker approaches were developed as a biomonitoring tool for some major mycotoxins.
Wednesday, November 13, 2013
Predictors of Obesity: The "Power" of the Omics
Diet and nutrient biomarkers should provide objective measures of dietary intake and nutritional status, as well as an integrated measure of intake, absorption and metabolism.
Friday, October 25, 2013
Optical Biosensors for Food Quality and Safety Assurance
With high specificity, convenience and quick response there is growing interest in biosensors for food safety testing.
Monday, October 07, 2013
Pesticide Residue Screening Using a Novel Artificial Neural Network Combined with a Bioelectric Cellular Biosensor
Researchers have developed and trained a customized feed forward artificial neural network for the classification of the pesticide groups, pyrethroids, carbamates, and organophosphates.
Friday, September 20, 2013
Effect of Trans Fatty Acid Intake on LC-MS and NMR Plasma Profiles
In this study, NMR and LC-MS untargeted metabolomics has been used as an approach to explore the impact of trans fatty acid intake on plasma metabolites.
Tuesday, August 13, 2013
Low Density Solvent-Based Dispersive Liquid-Liquid Microextraction for the Determination of Synthetic Antioxidants in Beverages by HPLC
In this study, researchers propose a method that could be used for monitoring as well as controlling the synthetic antioxidants in aqueous food samples.
Wednesday, July 31, 2013
What's New in the Diagnosis and Management of Food Allergy in Children?
With the increasing worldwide prevalence of food allergy comes a renewed impetus for improving diagnosis and management.
Monday, June 24, 2013
Genetically Modified Crops and Food Security
Although GM crops are not a panacea for the problems of hunger and malnutrition, evidence suggests that GM crops can be an important component in a broader food security strategy.
Monday, June 24, 2013
Use of FT-IR Spectra and PCA to the Bulk Characterization of Cell Wall Residues of Fruits and Vegetables Along a Fraction Process
The study has demonstrated that discrimination between the cell wall residues of fruits and vegetables is possible with the use of FT-IR spectra analysed using the PCA method.
Thursday, May 16, 2013
Scientific News
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
Kitchen Utensils Can Spread Bacteria Between Foods
In a recent study researchers found that produce that contained bacteria would contaminate other produce items through the continued use of knives or graters—the bacteria would latch on to the utensils commonly found in consumers' homes and spread to the next item.
GMO Food Animals Should be Judged by Product, Not Process
In a world with a burgeoning demand for meat, milk and eggs, regulatory policies around the use of biotechnologies in agriculture need to be based on the safety and attributes of those foods rather than on the methods used to produce them, says a UC Davis animal scientist.
Acetaldehyde and Formaldehyde Content in Foods
Korean researchers have determined the content of the toxic and carcinogenic aldehydes, acetaldehyde and formaldehyde, in a variety of food groups.
Increasing Vitamin D Supplementation
New study from ETH Zurich finds that elderly women should consume more vitamin D than previously recommended during the winter months.
IARC Monographs Evaluate Consumption of Red Meat and Processed Meat
Processed meat eaten daily increases the risk of colorectal cancer by 18%.
Nanoparticles in Foods Raise Safety Questions
Nanoparticles can make foods like jawbreaker candies brighter and creamier and keep them fresh longer. But researchers are still in the dark about what the tiny additives do once inside our bodies.
Arsenic Found in Many U.S. Red Wines
A new University of Washington study that tested 65 wines from America’s top four wine-producing states — California, Washington, New York and Oregon — found all but one have arsenic levels that exceed what’s allowed in drinking water.
Viruses Join Fight Against Harmful Bacteria
Engineered viruses could combat human disease and improve food safety.
Plastic for Dinner
Roughly a quarter of the fish sampled from fish markets in California and Indonesia contained man-made debris according to a study from the University of California, Davis, and Hasanuddin University in Indonesia.

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos