The MaxSignal® Boldenone ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Boldenone in fish, shrimp, meat and urine. This kit offers high sensitivity with rapid, cost effective sample prep protocols. The MaxSignal® Boldenone ELISA Test Kit is a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample. Boldenone, also known as Equipoise, Ganabol, Equigan or Ultragan, is an anabolic steroid with a low androgenic potency developed for veterinary use. It is given to horses for therapeutic purposes or to illegally improve their performance. Boldenone is also used in cattle and pigs for growth promoting purposes and to increase muscular tissues, because this molecule raises protein synthesis, appetite and stimulates the release of erythropoietin in the kidneys. The use of boldenone is forbidden in the EU. In the US, it is not indicated for use in humans and is only available through veterinary clinics. This kit is manufactured to the international quality standard ISO 9001:2008.