Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


MiraMas™ Kit

Product Description
The MiraMas™ Kit is designed to create cDNA libraries for qPCR detection of microRNAs and other small RNAs. The method is based on ligation of a 5’- adenylated/3’ blocked oligonucleotide adapter (Adenylated 3’ Ligation Adapter) to the 3’ ends of the small RNAs using RNA Ligase, followed by reverse transcription with M-MLV Reverse Transcriptase to convert the small RNAs to cDNA templates for qPCR. The ligated adapter provides the binding site for the reverse transcription (RT) primer. After the second-strand cDNA is synthesized by extension of a microRNA-specific Forward PCR primer, the same adapter sequence serves as binding site for the Reverse PCR primer. Use of an adenylated adapter is advantageous because it streamlines the work-flow and leads to increased specificity for producing the desired reaction products, with fewer unwanted side products. The MiraMas protocol uses a single-tube format for ligation, reverse transcription, and subsequent dilution of the cDNA library. The MiraMas Kit allows users great flexibility in formatting their experiments, in that the number of samples that can be processed is scaled to the amount of sample RNA used in the reactions. Depending on the amount of sample RNA, the kit can be used to produce cDNA libraries from 30 to 150 samples. Each cDNA library can be used for detection of tens to hundreds of target microRNAs. The kit includes enough Universal Reverse Primer for 1,000 real-time PCRs.
Product MiraMas™ Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 5208
Quantity 30 to 150 cDNA libraries
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
Process Contaminants in Vegetable Oils and Foods
Glycerol-based process contaminants found in palm oil, but also in other vegetable oils, margarines and some processed foods, raise potential health concerns for average consumers of these foods in all young age groups, and for high consumers in all age groups.
Apricot Kernels Pose Risk of Cyanide Poisoning
Eating more than three small raw apricot kernels, or less than half of one large kernel, in a serving can exceed safe levels. Toddlers consuming even one small apricot kernel risk being over the safe level.
Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
First DNA Vaccine in the EU Recommended for Use in Salmon
Clynav to protect Atlantic salmon from serious infectious disease.
Grant to Fund Million Peaks Project
The European Research Council (ERC) has awarded a prestigious Advanced Grant to Prof. Peter Schoenmakers, Prof. Albert Polman and Prof. Huib Bakker, all three of whom work at the University of Amsterdam (UvA).
Finding Inorganic Arsenic in Foodstuffs
A new European standard method to determine the content of inorganic arsenic in foodstuffs has been developed at the National Food Institute, Technical University of Denmark.
Effective Identification of Low-Gliadin Wheat Lines
Researchers have demonstrated the use of NIRS to identify low-gliadin wheat lines.
Virus Causing Tilapia Die-Offs Identified
Discovery of the virus causing Tilapia die-offs in Israel and Ecuador points the way to protecting a fish that feeds multitudes.
Interactive Maps Reveal Global Obesity
World’s obese population hits 640 million, according to largest ever study.
Eating Green Could be in Your Genes
Genetic variation uncovered that has evolved in populations that have historically favored vegetarian diets, such as in India, Africa and parts of East Asia.

SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!