Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


MiraMas™ Kit

Product Description
The MiraMas™ Kit is designed to create cDNA libraries for qPCR detection of microRNAs and other small RNAs. The method is based on ligation of a 5’- adenylated/3’ blocked oligonucleotide adapter (Adenylated 3’ Ligation Adapter) to the 3’ ends of the small RNAs using RNA Ligase, followed by reverse transcription with M-MLV Reverse Transcriptase to convert the small RNAs to cDNA templates for qPCR. The ligated adapter provides the binding site for the reverse transcription (RT) primer. After the second-strand cDNA is synthesized by extension of a microRNA-specific Forward PCR primer, the same adapter sequence serves as binding site for the Reverse PCR primer. Use of an adenylated adapter is advantageous because it streamlines the work-flow and leads to increased specificity for producing the desired reaction products, with fewer unwanted side products. The MiraMas protocol uses a single-tube format for ligation, reverse transcription, and subsequent dilution of the cDNA library. The MiraMas Kit allows users great flexibility in formatting their experiments, in that the number of samples that can be processed is scaled to the amount of sample RNA used in the reactions. Depending on the amount of sample RNA, the kit can be used to produce cDNA libraries from 30 to 150 samples. Each cDNA library can be used for detection of tens to hundreds of target microRNAs. The kit includes enough Universal Reverse Primer for 1,000 real-time PCRs.
Product MiraMas™ Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 5208
Quantity 30 to 150 cDNA libraries
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
How To Keep Your Rice Arsenic-Free
Researchers at Queen’s University Belfast have made a breakthrough in discovering how to lower worrying levels of arsenic in rice that is eaten all over the world.
Pesticide Found in 70 Percent of Massachusetts’ Honey Samples
New Harvard University study says that the pesticide commonly found in honey samples is implicated in Colony Collapse Disorder.
Printed "Smart Cap" Detects Spoiled Food
It might not be long before consumers can just hit “print” to create an electronic circuit or wireless sensor in the comfort of their homes.
Red Wine Antioxidant May Provide New Cancer Therapy Options
Resveratrol and quercetin, two polyphenols that have been widely studied for their health properties, may soon become the basis of an important new advance in cancer treatment,
New Research will Show How the Environment Could Change the Way We Eat
A new study funded by the Wellcome Trust will investigate how environmental changes over the next 20-30 years may impact the way we eat, in the UK and worldwide.
Blue LEDs Can be Used to Preserve Food
Blue light emitting diodes (LEDs) have strong antibacterial effect on major foodborne pathogens and can be used as a chemical-free food preservation method, a new study has found.
FDA Declares Trans Fatty Acids Unsafe for Consumption
TFAs are widely recognized as the most harmful fat with regard to causing cardiovascular disease (CVD).
Fat, Sugar Cause Bacterial Changes that may Relate to Loss of Cognitive Function
A study has indicated that both a high-fat and a high-sugar diet, compared to a normal diet, cause changes in gut bacteria that appear related to a significant loss of "cognitive flexibility," or the power to adapt and adjust to changing situations.
How Anthrax Spores Grow in Cultured Human Tissues
New findings to help predict risk and outcomes of anthrax attacks.
Food Research at the Microscale
Thermal stage microscopy allows food science microscopists to analyze samples under a range of conditions.

SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!