Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


MiraMas™ Kit

Product Description
The MiraMas™ Kit is designed to create cDNA libraries for qPCR detection of microRNAs and other small RNAs. The method is based on ligation of a 5’- adenylated/3’ blocked oligonucleotide adapter (Adenylated 3’ Ligation Adapter) to the 3’ ends of the small RNAs using RNA Ligase, followed by reverse transcription with M-MLV Reverse Transcriptase to convert the small RNAs to cDNA templates for qPCR. The ligated adapter provides the binding site for the reverse transcription (RT) primer. After the second-strand cDNA is synthesized by extension of a microRNA-specific Forward PCR primer, the same adapter sequence serves as binding site for the Reverse PCR primer. Use of an adenylated adapter is advantageous because it streamlines the work-flow and leads to increased specificity for producing the desired reaction products, with fewer unwanted side products. The MiraMas protocol uses a single-tube format for ligation, reverse transcription, and subsequent dilution of the cDNA library. The MiraMas Kit allows users great flexibility in formatting their experiments, in that the number of samples that can be processed is scaled to the amount of sample RNA used in the reactions. Depending on the amount of sample RNA, the kit can be used to produce cDNA libraries from 30 to 150 samples. Each cDNA library can be used for detection of tens to hundreds of target microRNAs. The kit includes enough Universal Reverse Primer for 1,000 real-time PCRs.
Product MiraMas™ Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 5208
Quantity 30 to 150 cDNA libraries
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Peanut Allergy Prevention Strategy is Nutritionally Safe
Early-life peanut consumption does not affect duration of breastfeeding or children’s growth and nutrition.
A Future Tool for Medicine, Food Safety
A new type of electronic sensor that might be used to quickly detect and classify bacteria for medical diagnostics and food safety has passed a key hurdle by distinguishing between dead and living bacteria cells.
Local Microbes Can Predict Wine’s Chemical Profile
Regionally distinctive groups of bacteria and fungi, associated with local climate and environmental conditions, may leave a very specific “fingerprint” on a wine’s chemical composition, report University of California, Davis, researchers who collaborated on a new study with two Napa Valley wineries.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Genetically Engineered Crops Are Safe
Distinction between genetic engineering and conventional plant breeding becoming less clear, says new report on GE crops.
Developing Non-Allergenic 'Super' Peanuts
Scientists from The University of Western Australia have joined a global research team that have identified genes in peanuts that when altered will be able to prevent an allergic response in humans.
Checking the Quality of Chocolate With Ultrasound
The method, developed by researchers from KU Leuven, could save the chocolate industry a lot of time and money.
Detecting Fake Parmesan Cheeses
Scientists report on a way to catch adulteration of the regional artisanal products.
Cancer-Fighting Properties Of Horseradish Revealed
Horseradish contains cancer-fighting compounds known as glucosinolates. Glucosinolate type and quantity vary depending on size and quality of the horseradish root. For the first time, the activation of cancer-fighting enzymes by glucosinolate products in horseradish has been documented.

SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!