Corporate Banner
Satellite Banner
Food & Beverage Analysis
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Feeding Babies Egg and Peanut May Prevent Food Allergy
The new analysis pools all existing data, and suggests introducing egg and peanut at an early age may prevent the development of allergy.
Food Colour Re-Evaluation Milestone
The re-evaluation of titanium dioxide marks the completion of the EFSA's re-evaluation of all food colours permitted for use in the EU before 2009.
Risks in Your Food
Researchers have developed a method to reliably detect allergenic substances in foods.
Dietary Selenium Content Linked to Cancer
Researchers have shown higher blood selenium levels are associated with reduced liver cancer risk.
Sensor Could Help Fight Bacterial Infections
The sensor can detect E.coli bacteria in 15-20 minutes over a wide temperature range, offering a fast and cost effective tests.
Chemical in Plastics Linked to Genital Abnormalities
Researchers have linked an endocrine-disrupting chemical to reproductive organ abnormalities in children.
Sharks Contain High Levels of Neurotoxins Linked to Alzheimer’s
Research team suggests restricting shark consumption to protect human health as shark fins & meat contain high levels of neurotoxins.
Investigating Bacteria in Raw Milk
A microbial study of milk trucks aims to improve dairy food safety and quality.
FDA Isolates Hepatitis A Outbreak Origin
The FDA and CDC are aiding the Hawaii Department of Health investigation into hepatitis A virus (HAV) infections linked to imported scallops.
MRSA – Just Add Salt
Scientists have discoved a new way to attack Staphylococcus aureus through salt content mechanisms
Scroll Up
Scroll Down

Automated Sample Preparation of 25-OH-Vitamin D3/D2 from Serum with the AC Extraction Plate and the Tecan Freedom EVO® for the quantification by LC-MS/MS
Bookmark and Share

Tecan Group Ltd.

The current methods for sample preparation include protein precipitation (‘protein crash’), solvent extraction such as liquid-liquid extraction (LLE) and solid-phase extraction (SPE). 

However, all these methods require at least one labor intensive and time-consuming step such as filtration, centrifugation or solvent evaporation. In addition, these steps do not allow straightforward automation of the sample preparation process which is highly desirable in order to cope with the increasing Vitamin D sample load experienced by clinical laboratories. 

The Tecan AC Extraction Plate simplifies the sample preparation step in such a way that no centrifugation, filtration or solvent evaporation steps are needed. In fact the sample preparation procedure is reduced to simple pipetting and shaking. These pipetting and shaking steps were automated on a Tecan Freedom EVO liquid handling system. 

In this application note an approach is described which overcomes the difficulties described above. 


Further Information


SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!