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  Events - November 2013

1 day Sample preparation and quality control

20 Nov 2013 - 20 Nov 2013 - TATAA Biocenter, Odinsgatan 28, Gothenburg

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Target audience: Beginners to Medium experienced qPCR users

Entrance qualifications: Basic Molecular biology or similar

Description: One of the most important requirements to get good results from qPCR experiments is to have a template of good quality. In most cases this means having an efficient sample preparation. This course module is focused on extraction of RNA and DNA to be used as template in qPCR and reverse transcription reactions. It also includes the important quality control steps needed to asure reliable results. The course contains:

  • Overview of nucleic acid extraction methods
  • How to properly determine the concentration of purified nucleic acids
  • Storage of biological samples and purified nucleic acids
  • Quality control of purified material
  • Integrity of purified RNA
  • How to test for the presence of inhibitors

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