Corporate Banner
Satellite Banner
Forensic Science & Clinical Toxicology
Scientific Community
 
Become a Member | Sign in
Home>Events>This Event
  Events - December 2013


3 days Hands-on qPCR

02 Dec 2013 - 04 Dec 2013 - TATAA Biocenter, Prague



Bookmark and Share


3 DAYS HANDS-ON QPCR

Target audience: Beginners to Medium experienced qPCR users

Entrance qualifications: Basic Molecular biology or similar

Description: The basic real-time qPCR course. You will aquire a comprehensive overview of the possibilities with real-time PCR, how to use it and how to analyze the results. The course contains:

Day 1

Basic PCR theory
The theory of real-time PCR
Applications and possibilities of qPCR. Comparison of qPCR with regular PCR.
Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons, etc)
Different instrument platforms and their typical uses
Primer design
The problem of primer-dimer formation and how to minimize them
Probe design
Experimental design and optimization
Basic data handling and analysis

Day 2

Introduction to quantification principles
Quantification strategies, uses and limitations
Calculations using different relative quantification methods
Strategies for normalization of qPCR data
In situ calibration for compensation of inhibition in samples
Absolute quantification

Day 3

Sample Preparation (Extraction of RNA and DNA)
Principles of RT
Priming methods for RT
RT optimization
The MIQE guidelines
Group discussion, bring your own questions



Further information
Scientific News
Peer Review is in Crisis, But Should be Fixed, Not Abolished
After the time to get the science done, peer review has become the slowest step in the process of sharing studies, and some scientists have had enough.
Rapid Identification of Illicit Designer Drugs
New technique can identify commonly used illicit compounds in as little as 15 seconds.
Trace Evidence Database for Forensic Investigators
Central database aims to bring together disparate evidence logs into a common resource for forensic scientists.
Forensics is Boosting the Battle Against Wildlife Trade
From rapid genetic analysis to spectrography, the new advances in forensics offer promise in stopping the trafficking in endangered species.
3D Imaging in Forensics
New portable, user-friendly crime-scene forensics tech will take high-resolution 3-D images of shoeprints, tire tracks in snow and soil.
A Simple Tool for Toxicological Analysis
In this study, GC-MS is used for the determination of clozapine, and five antidepressants in human plasma, serum and whole blood.
Identification of Individual Red Blood Cells by Raman Microspectroscopy
In this study, Raman Microspectroscopy was used to identify individual red blood cells.
3D-Printing in Science: Conference Co-Staged with LABVOLUTION
LABVOLUTION 2017 will have an added highlight of a simultaneous conference, "3D-Printing in Science".
Sequencing Soviet Anthrax
Next generation genomic analysis used to probe the former USSR’s biological weapons program.
Identifying People Using Human Hair Proteins
In an important breakthrough for the forensic science community, researchers have developed the first-ever biological identification method that exploits the information encoded in proteins of human hair.
Scroll Up
Scroll Down
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!