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Home>Events>This Event
  Events - December 2013

3 days Hands-on qPCR

02 Dec 2013 - 04 Dec 2013 - TATAA Biocenter, Prague

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Target audience: Beginners to Medium experienced qPCR users

Entrance qualifications: Basic Molecular biology or similar

Description: The basic real-time qPCR course. You will aquire a comprehensive overview of the possibilities with real-time PCR, how to use it and how to analyze the results. The course contains:

Day 1

Basic PCR theory
The theory of real-time PCR
Applications and possibilities of qPCR. Comparison of qPCR with regular PCR.
Review of currently available detection technologies (SYBR Green I, hydrolysis probes, Molecular Beacons, etc)
Different instrument platforms and their typical uses
Primer design
The problem of primer-dimer formation and how to minimize them
Probe design
Experimental design and optimization
Basic data handling and analysis

Day 2

Introduction to quantification principles
Quantification strategies, uses and limitations
Calculations using different relative quantification methods
Strategies for normalization of qPCR data
In situ calibration for compensation of inhibition in samples
Absolute quantification

Day 3

Sample Preparation (Extraction of RNA and DNA)
Principles of RT
Priming methods for RT
RT optimization
The MIQE guidelines
Group discussion, bring your own questions

Further information
Scientific News
Questioning the Validity of Forensic DNA Match Statistic
Fifteen years of criminal cases with affected mixture evidence.
Study Raises Questions About DNA Evidence
University of Indianapolis researchers say contamination through secondary transfer of material could implicate the innocent or help the guilty go free.
'Forensic Toolkit’ to Improve Evidence Detection and Analysis
Students from The University of Dundee have been developing a forensic `toolkit’ that will allow investigators to determine the age of fingerprints, detect traces of steel on bone from stabbings, and produce a biosensitive spray that can reveal traces of bodily fluids at crime scenes.
Glowing Fingerprints to Fight Crime
A CSIRO scientist who had his home broken into has developed a new crime scene identification technique to help fingerprint criminals.
Forensic Facial Examiners Can Be Near Perfect
In what might be the first face-off of its kind, trained forensics examiners from the FBI and law enforcement agencies worldwide were far more accurate in identifying faces in photographs than nonexperts and even computers.
Ancestral Background Can Be Determined By Fingerprints
A proof-of-concept study finds that it is possible to identify an individual’s ancestral background based on his or her fingerprint characteristics – a discovery with significant applications for law enforcement and anthropological research.
CSI -- On The Metabolite's Trail
Bioinformaticians at the University of Jena make the most efficient search engine for molecular structures available online.
People Emit Their Own Personal Microbial Cloud
We each give off millions of bacteria from our human microbiome to the air around us every day, and that cloud of bacteria can be traced back to an individual.
Blood, Teeth Samples Predict a Criminal's Age
Forensic biomedical scientists from KU Leuven have developed a test to predict individuals’ age on the basis of blood or teeth samples. This test may be particularly useful for the police, as it can help track down criminals or identify human remains.
Contactless Fingerprint Technology is Coming
Quickly moving through security checkpoints by showing your hand to a scanner seems straight out of science fiction, but NIST is working with industry to bring fast, touchless fingerprint readers out of the lab and into the marketplace.
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