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Instrumental Investigations: A Laboratory Manual of Forensic Analytical Chemistry
Robert Q. Thompson

A laboratory manual for undergraduate analytical and forensic chemistry courses

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Determination of C2-C12 Aldehydes in Water by SPME Arrow On-Fiber Derivatization and GC/MS
Peter Egli, Beat Schilling, Guenter Boehm, Kai Schueler

A method applying SPME Arrow extraction and on-fiber derivatization for the quantitation of C2-C12 aldehydes in water by GC/MS is described.

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Assessing Diversity in Cassava through the Application of Metabolomics
Margit Drapala, Elisabete Carvalhoa, Laura Perez-Fonsa, Elliott Pricea, L. Augusto Becerra Lopez-Lavalleb, Paul D. Frasera

In the present study metabolomic platforms have been established for Cassava and used to assess the biodiversity present in Cassava germplasm collections and elucidate underlying biochemical mechanisms associated with traits of interest.

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A KNIME Pipeline for the Analysis of GC-MS Data in Metabolomics
Sonia Liggi1, Maria Laura Santoru1, Cristina Piras1, Antonio Murgia2, Pierluigi Caboni2, Luigi Atzori1

A KNIME pipeline was developed to perform pre-processing of GC-MS data in an automated way and applied to a Inflammatory bowel diseases case study

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PredRet: Prediction of Retention Time by Direct Mapping between Multiple Chromatographic Systems
Jan Stanstrup, Steffen Neumann, Urška Vrhovšek

Retention time (RT) information is under-utilized in LC-MS based metabolomics and sharing of RTs between systems is not currently possible. PredRet is a new system that allows highly accurate mapping and prediction of RTs between LC systems.

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Profiling of metabolomic changes induced by testosterone esters in pig plasma and urine
Kamil Stastny, Martin Faldyna, Milan Franek

In this study, metabolic fingerprinting to discriminate between pigs treated with 17ß-testosteron esters and control animals has been investigated. Multivariate statistical analysis showed significant metabolic differences between test and control groups on day 28 after aplication of the testosterone hormonal preparation.

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Sample Stabilization and Purification Devices for Superior Forensic DNA Storage, Purification and Profiling
Geoffrey Routh,& Bassam El-Fahmawi

The tools used for evidentiary sample collection are often challenged in criminal cases. Maintaining the integrity of evidentiary samples continues to be a major challenge - especially when samples stay in transit during transportation where sample integrity is highly compromised. Thus, there is a crucial need for a cost-effective, efficient DNA collection and storage system that maintain sample integrity from collection until processing, while providing a means to ensure proper chain of custody

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A Simple Plate Based Assay Using pH Sensor Dye to Screen for Internalizing Antibody
Nidhi Nath, Becky Godat, Cesear Corona, Chad Zimprich, Mark McDougall, Poncho Meisenheimer, Marjeta Urh

Receptor mediated internalization is a key mechanism of action (MOA) for antibody drug conjugates (ADCs). However, current methods of studying antibody internalization have several limitations including: 1) A multistep process not suitable for screening; 2) Low signal-to-background ratios; 3) Not suitable for kinetic measurements. We have developed a method that mitigates problems associated with traditional internalization assays.

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An IgG Cleaving Protease from S equi with Improved Activity Against Mouse IgGs
Chris Hosfield, Philip Compton, Luca Fornelli, Paul Thomas, Neil L. Kelleher, Michael Rosenblatt & Marjeta Urh

Here we have expressed and purified a modified recombinant IdeZ, and show that it has significantly improved activity against mouse IgG2a and IgG3 subclasses when compared to IdeS. We also demonstrate the use of IdeZ in LC-MS workflows for human and mouse IgG characterization.

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Minimizing Carry-over for High Throughput Analysis
Christian Berchtold1, Reto Bolliger2, Guenter Boehm2, Götz Schlotterbeck1

Minimal carry-over is a prerequisite for high throughput analysis. However, minimized carry-over and cycle time are competing and a careful optimization is mandatory. In this study the influence of wash conditions on carry-over of various compounds was investigated. A strategy to minimize carry-over was developed. The influences of different wash tasks were investigated. Finally the contribution of different system components such as injector valve or column was studied.

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Scientific News
World’s Oldest Human Footprints Investigated
Bournemouth University researchers investigate world’s oldest human footprints with software designed to decode crime scenes.
Beating the Backlog in Criminal Investigations
Andrew Sheldon, Chief Technical Officer at UK Digital Forensics specialists Evidence Talks, says there is a way to beat the backlog in processing digital evidence.
Bringing the Lab to the Crime Scene
Developing a miniature mass spectrometer to allow instant analysis of evidence.
Forensic Botany Uses Plant DNA to Trace Crimes
Sam Houston State University is advancing the field of forensic botany with the publication of two recent studies that use marijuana DNA to link drug supplies and pollen DNA to aid in forensic investigations.
First Gene for Grey Hair Found
The first gene identified for greying hair has been discovered by an international UCL-led study, confirming greying has a genetic component and is not just environmental. - See more at: https://www.ucl.ac.uk/news/news-articles/0316/010316-first-grey-hair-gene-discovered#sthash.gD0shNNC.dpuf
Determining 'Patterns' for Bones Left on Ground Surfaces
For the first time, researchers have determined a signature of changes that occur to human remains, specifically bones, left outside in the New England environment.
Forensics Close in on Footwear Analysis
First it was your fingerprint that gave the game away and then DNA analysis transformed forensic science. But ‘watch your step’ because an expert in the School of Physics and Astronomy at The University of Nottingham has developed a new technique which could lead to a ‘step change’ in forensic footwear imaging.
Characterizing the Smell of Death
New research reveals the odor profile of decaying bodies.
New Forensic Methods for Human DNA Cases
Sam Houston State University was awarded a grant from the National Institute of Justice to develop and test the best possible sample preparation methods for skeletal and decomposing human remains using emerging, next-generation DNA technology to identify missing persons or victims of mass disasters.
Portable Kit Can Recover Traces of Chemical Evidence
A chemist at the National Institute of Standards and Technology (NIST) has developed a portable version of his method for recovering trace chemicals such as environmental pollutants and forensic evidence including secret graves and arson fire debris.
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