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The Analysis of Common Drugs of Abuse in an Oral Fluid Matrix using a New Q TRAP® Platform
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SCIEX

Testing in oral fluid matrices requires monitoring compounds using a simple sample preparation protocol, allowing for the analysis to be performed in a timely fashion, while at the same time providing excellent data quality. 

This application note develops a fast, robust, and reliable method for the detection of 15 compounds in an oral fluid matrix. The LCMS/ MS methods were performed using Multiple Reaction Monitoring (MRM) along with fast positive negative switching detecting all compounds with Limits of Quantitation (LOQ) in-line with the current recommended cutoffs. Throughput was also considered by using the MPX™-2 high throughput HPLC system where an increase in sample throughput of 142% was observed. In addition to the above the quantitative positive negative switching capabilities were assessed and it was found to work excellent on the QTRAP® 4500 system. 


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