Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, now provides xGen™ Lockdown™ Probes individually synthesized 60-120 nucleotide 5’-biotinylated capture oligonucleotides designed to enable cost-effective, high depth sequencing and analysis of specific regions of the genome.
xGen Lockdown Probes are ideal for target enrichment and custom panel development, and capture sets can be easily expanded. Available at three different scales (mini – 2 pmole, standard – 20 pmole, and XL – 200 pmole), users can design their initial capture panels using the mini scale and extend them by progressively supplementing with more probes. As a result, it is quick and simple to transition to higher volume applications.
Whether used with custom capture panels, or for supplementing existing panels, xGen Lockdown Probes provide approximately 5000-fold enrichment with high coverage over targeted regions, and minimal GC bias. Furthermore, the probes can be added to array-derived RNA baits to enhance coverage of GC-rich targets, such as first exons. Users can therefore improve capture performance by rescuing drop-out regions of array-derived exome capture, improve the uniformity of coverage, discover additional genetic mutations, or improve coverage of specific regions of interest without redesigning a new panel.
Each xGen Lockdown Probe is individually synthesised and quality assessed via mass spectroscopy, providing high levels of performance. They are supplied in 96- or 384-well plates, or as pools of up to 2000 oligos. Shipped within 7-10 business days, users can get a quick start on their projects, facilitating the transition from discovery to clinical application.