Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

The Microplate Reader Durable Enough to Withstand Changes in Gravity

Published: Wednesday, October 17, 2012
Last Updated: Wednesday, October 17, 2012
Bookmark and Share
Studying the physiological effects of gravity fluctuations on plants, researchers at the University of Tuebingen in Germany use BMG LABTECH’s POLARstar microplate reader in parabolic flights.

It is known that light and gravity are the two most important factors in helping plants determine where top and bottom are. It is easy to eliminate light to see what effect it has on plants, but trying to investigate changes in gravity prove a little more difficult. By measuring calcium and hydrogen peroxide in plant cells with the durable POLARstar microplate reader, researchers show the effects of gravity on plants during consecutive parabolic flights.

During a parabolic flight, different stages of gravitation are obtained ranging from hypergravity (about 1.8no-gravity-polarstar.gif g) to microgravity (about 10-3 g). In one flight, 31 consecutive parabolas are flown. Novespace, a company from France has been performing these flights for over ten years. The gravitational effects of the parabolic flight can be seen in a simulated video (http://www.novespace.fr/fr,popup,parabole.html) and in a real video (http://www.youtube.com/watch?v=Lhu198E8z2U). Normal gravity is designated as 1g and on the ascent of the parabola the gravity goes to about 1.8 g (80% higher than normal). Then on the descent of the parabola when the experiments are performed, the period of microgravity lasts for about 22 seconds.

To investigate the signaling pathway from the stimulus (no gravity) to the response (e.g. activation or deactivation of the expression of certain genes) the level of two important second messengers, Ca2+ and hydrogen peroxide, were monitored. Two transgenic cell lines derived from Arabidopsis thaliana expressing either Cameleon (a calcium sensor) or HyPer (an H2O2 sensor) were used as well as a wild type. The calcium and hydrogen peroxide measurements were performed on a fixed POLARstar OPTIMA in kinetic mode during the whole time of the parabolic flight.

Results show that the calcium levels increased with microgravity and decreased afterwards, signifying an effect on calcium flux due to changes in gravity. Further parabolic flights with the POLARstar OPTIMA and with a new PHERAstar FS are in preparation. Some of the results can be found online (http://www.bmglabtech.com/customer-focus/parabolic-flights.cfm). Corresponding experiments were also recently performed at higher gravity levels with the robust POLARstar microplate reader. Using the Hyper-g centrifuge at ZARM (The Center of Applied Space Technology and Microgravity) in Bremen Germany, the entire unit has been routinely centrifuged up to 10g, further demonstrating the robustness of the instrumentation.  

The POLARstar microplate reader from BMG LABTECH is a multimode plate reader that can measure fluorescence, luminescence, absorbance, and fluorescence polarization. Its robust steel housing and perfectly engineered machinery allow it to perform even under extreme conditions such as high and low levels of gravity.

These experiments were financed by a grant from the Deutsches Zentrum für Luft- und Raumfahrt (DLR; 50WB0723). For more information about the Tuebingen research group please have a look here:
http://www.bbp.uni-tuebingen.de/index.php5?object=Content.ResearchTopics.GravitationBiology.

BMG LABTECH is a leading developer and global manufacturer of microplate reader instrumentation with a wide range of measurement methods. Microplate readers are used in the pharmaceutical and biotech industries, as well as in academic research establishments, for both basic research analysis and High Throughput Screening. BMG LABTECH focuses solely on microplate readers and offers the most diverse selection of optical detection systems in conjunction with integrated liquid handling equipment. For more information visit: www.bmglabtech.com

BMG LABTECH, bringing the future of microplate reader technology to you today.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Monovar Drills Down Into Cancer Genome
Rice, MD Anderson develop program to ID mutations in single cancer cells.
Autism and Cancer Share a Remarkable Number of Risk Genes
Researchers with the UC Davis Comprehensive Cancer Center, MIND Institute identify more than 40 common genes.
Number Of Known Genetic Risk Factors For Endometrial Cancer Doubled
An international collaboration of researchers has identified five new gene regions that increase a woman’s risk of developing endometrial cancer, one of the most common cancers to affect women, taking the number of known gene regions associated with the disease to nine.
Genetic Variant May Help Explain Why Labradors Are Prone To Obesity
A genetic variation associated with obesity and appetite in Labrador retrievers – the UK and US’s favourite dog breed – has been identified by scientists at the University of Cambridge. The finding may explain why Labrador retrievers are more likely to become obese than dogs of other breeds.
How Scientists Use DNA to Track Disease Outbreaks
They’re the top questions on everyone’s mind when a new disease outbreak happens: where did the virus come from? When did this happen? How long has it been spreading in a particular country or group of people?
Genetic Risk Factors of Disparate Diseases Share Similar Biological Underpinnings
Penn Institute for Biomedical Informatics and colleagues identify "roadmap" of disease mechanisms to identify candidate drug targets.
Stem Cells Know How to Unwind
Research led by the Babraham Institute with collaborators in the UK, Canada and Japan has revealed a new understanding of how an open genome structure supports the long-term and unrestricted developmental potential in embryonic stem cells.
Childhood Asthma Research Receives $2M
Research into the impact of a child’s upbringing and social and physical environments on the development of asthma will receive $2 million to tackle the condition that affects as many as one in three Canadians.
Five New Breast Cancer Genes Found
Discovery of mutations paves the way for personalised treatment of breast cancer.
Cell Transplant Treats Parkinson’s in Mice
A University of Wisconsin—Madison neuroscientist has inserted a genetic switch into nerve cells so a patient can alter their activity by taking designer drugs that would not affect any other cell.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!