Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Illinois State University Use NTA in the Development of Novel Bioanalytical Assays

Published: Friday, March 15, 2013
Last Updated: Friday, March 15, 2013
Bookmark and Share
Development of novel bioanalytical assays to detect nucleic acids, proteins, and whole viruses.

NanoSight reports on how Nanoparticle Tracking Analysis, NTA, is being used in the development of novel bioanalytical assays at Illinois State University.

The main application is the characterization of gold nanoparticles and differentiation between monodisperse samples from small numbers of aggregated materials.

Speeding up the detection and identification of viruses is one of the areas of new research of Illinois State University Assistant Professor of Chemistry Jeremy Driskell.

This has been recognized by the US Department of Defense in their recent award of a major grant. Reliable and accurate nanoparticle composition is important in such work.

Here, Dr Driskell describes how he has used various techniques.

Talking about his current work, Dr Driskell says "Our research group is focused on the development of novel bioanalytical assays which includes detection of nucleic acids, proteins, and whole viruses. While other groups aim to improve assay sensitivity or detection limits, our central focus is on reducing assay speed and complexity. We are currently using gold nanoparticles and gold filters to develop assays utilizing surface-enhanced Raman spectroscopy for detection. In the process of characterizing the gold nanoparticles and monitoring the modification steps required for the SERS assays, we found that particle sizing techniques such as Dynamic Light Scattering (DLS) and Nanoparticle Tracking Analysis (NTA) could also be used for assay readout. This finding and application is detailed in our recent publication in the Analyst."

Dr Driskell continued: "We began by using DLS to characterize gold nanoparticles that we modify with Raman reporter molecules and antibodies. This was a simple means of detecting particle aggregation as a result of surface modification and is more sensitive than colorimetric detection, which we found was not useful for conditions that invoked slight aggregation. When we learned of NanoSight and NTA, we compared the data to DLS. For our purposes, NTA gave a much more accurate representation of the actual particle sizes in our solutions. DLS would frequently indicate aggregation of a nanoparticle population while NTA revealed few large aggregates while the majority of the particles remained monodisperse. Ultimately we concluded it was a better technique to give us a better understanding of nanoparticle composition (aggregates compared with individual particles) in terms of absolute numbers."


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Method Development to Estimate the Purity of Vesicle Preparations
Nanoparticle tracking analysis is used to estimate the purity of vesicle preparations at the Cardiff University School of Medicine.
Thursday, February 28, 2013
Duke University Uses NTA to Characterize "Nanoconstructs" for Biomedical Applications
NTA to characterize metal nanoparticle construct materials for use in biosensing, imaging and cancer therapy.
Friday, April 20, 2012
Scientific News
Light Signals from Living Cells
Fluorescent protein markers delivered under high pressure.
Counting Cancer-busting Oxygen Molecules
Researchers from the Centre for Nanoscale BioPhotonics (CNBP), an Australian Research Centre of Excellence, have shown that nanoparticles used in combination with X-rays, are a viable method for killing cancer cells deep within the living body.
Genomic Signature Shared by Five Types of Cancer
National Institutes of Health researchers have identified a striking signature in tumor DNA that occurs in five different types of cancer.
Crowdfunding the Fight Against Cancer
From budding social causes to groundbreaking businesses to the next big band, crowdfunding has helped connect countless worthy projects with like-minded people willing to support their efforts, even in small ways. But could crowdfunding help fight cancer?
Switch Lets Salmonella Fight, Evade Immune System
Researchers at the University of Illinois at Chicago have discovered a molecular regulator that allows salmonella bacteria to switch from actively causing disease to lurking in a chronic but asymptomatic state called a biofilm.
Genetic Cause of Rare Allergy
Institute has identified a genetic mutation responsible for a rare form of inherited hives induced by vibratory urticaria.
Mitochondria Shown to Trigger Cell Ageing
An international team of scientists has for the first time shown that mitochondria, the batteries of the cells, are essential for ageing.
Cancer Cells Kill Off Healthy Neighbours
Cancer cells create space to grow by killing off surrounding healthy cells, according to UK researchers working with fruit flies.
Validating the Accuracy of CRISPR-Cas9
IBS Researchers create multiplex Digenome-seq to find errors in CRISPR-Cas9 processes.
Cancer Drug Target Visualized at Atomic Resolution
New study using cryo-electron microscopy shows how potential drugs could inhibit cancer.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!