Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

iTaq Universal One-Step Kits for RT-qPCR

Published: Wednesday, August 21, 2013
Last Updated: Wednesday, August 21, 2013
Bookmark and Share
Bio-Rad Laboratories, Inc. announced the launch of two iTaq universal one-step kits that deliver superior data precision and highly efficient RT-qPCR performance under most reaction conditions on any qPCR instrument.

The new iTaq universal one-step kits include the iTaq universal SYBR® Green one-step kit and the iTaq universal probes one-step kit. Both kits come as a 2x concentrated, ready-to-use reaction mix with iScript™ reverse transcriptase optimized for RT-qPCR. 

The one-step kits are universal due to their broad instrument compatibility and the ability to tolerate a wide range of reaction and protocol conditions. The multiple passive reference dyes included in their formulation are particularly useful for researchers using qPCR instruments that require ROX fluorescent dyes. In addition, the combination of RNase H+ iScript reverse transcriptase and antibody-mediated hot-start iTaq DNA polymerase in one reaction improves PCR efficiency, dynamic range, data precision, sensitivity, and specificity.

The iTaq universal SYBR® Green one-step kit includes SYBR® Green I dye and Bio-Rad’s patented RT inhibitor reducer. The iTaq universal probes one-step kit allows researchers to combine multiple targets in one well (multiplexing), improving data precision and reducing costs.

 Key benefits of the iTaq universal one-step kits include:

• Uncompromised data with fast cycling — obtain highly efficient data in 55 minutes, which includes the reverse transcription reaction, using fast cycling conditions

• Optimized buffer formulation — achieve efficient amplification of challenging templates, cell lysates, and difficult target sequences 

• Instant activation — complete polymerase activation in 30 seconds for greater reaction specificity and superior data

Bio-Rad’s iTaq universal one-step kits can be used for gene expression analysis, mutation detection, genetically modified organism (GMO) characterization, genetic profiling, and other genomics applications.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
How Scientists Use DNA to Track Disease Outbreaks
They’re the top questions on everyone’s mind when a new disease outbreak happens: where did the virus come from? When did this happen? How long has it been spreading in a particular country or group of people?
Genetic Risk Factors of Disparate Diseases Share Similar Biological Underpinnings
Penn Institute for Biomedical Informatics and colleagues identify "roadmap" of disease mechanisms to identify candidate drug targets.
Stem Cells Know How to Unwind
Research led by the Babraham Institute with collaborators in the UK, Canada and Japan has revealed a new understanding of how an open genome structure supports the long-term and unrestricted developmental potential in embryonic stem cells.
Childhood Asthma Research Receives $2M
Research into the impact of a child’s upbringing and social and physical environments on the development of asthma will receive $2 million to tackle the condition that affects as many as one in three Canadians.
Five New Breast Cancer Genes Found
Discovery of mutations paves the way for personalised treatment of breast cancer.
Cell Transplant Treats Parkinson’s in Mice
A University of Wisconsin—Madison neuroscientist has inserted a genetic switch into nerve cells so a patient can alter their activity by taking designer drugs that would not affect any other cell.
Understanding Female HIV Transmission
Glowing virus maps points of entry through entire female reproductive tract for first time.
Genetic Markers Influence Addiction
Differences in vulnerability to cocaine addiction and relapse linked to both inherited traits and epigenetics, U-M researchers find.
A lncRNA Regulates Repair of DNA Breaks in Breast Cancer Cells
Findings give "new insight" into biology of tough-to-treat breast cancer.
Detection of HPV in First-Void Urine
Similar sensitivity of HPV test on first void urine sample compared to cervical smear.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!