Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

InSphero Publishes 3D Tumor-Stroma Model for Non-Small Cell Lung Cancer

Published: Thursday, April 24, 2014
Last Updated: Thursday, April 24, 2014
Bookmark and Share
PLOS One article delivers promising co-culture method to mimic tumor microenvironment, demonstrating potential for biomarker and drug discovery.

InSphero AG, working with researchers at the Medical University Innsbruck, has published a study highlighting development of a novel 3D cell culture model for non-small cell lung cancer (NSCLC), one of the leading causes of cancer deaths in men and women worldwide. The findings, published March 24 in PLOS One, used InSphero's Grav1tyPLUSTM hanging drop  platform to form 3D tumor spheroid co-cultures comprised of a NSCLC tumor cell line and lung-derived fibroblasts. The results showed how both lung cancer cells and stromal cells (lung fibroblasts) behave differently when grown together in 3D than when grown alone. 

The study was initiated in response to the recent failure of several targeted therapies for NSCLC in clinical trials, therapies which were supported by promising in vitro data. To achieve a better in vitro model, researchers used the NSCLC cell lines A549 or Colo699 to create 3D tumors in hanging drops, with or without the lung fibroblast cell line SV80. The tumors were then analyzed for viability, morpology, and expression of different phenotypic markers using immunohistochemistry (IHC) and other methods. Most notable were changes in the tumor cells during co-culture that indicated an epithelial to mesenchymal transition (EMT), as evidenced by an increase in vimentin protein expression, and a decrease in the epithelial cell adhesion protein E-cadherin. Also of interest was the expression of alpha smooth muscle actin (a-SMA), a marker of cancer-associated fibroblasts, in the SV80 fibroblasts only when co-cultured with A549 cells. 

Dr. Jens Kelm, Chief Scientific Officer and co-founder of InSphero AG was co-author on the manuscript He states the 3D co-culture model should improve drug efficacy testing by removing some of the bias inherent in current 2D in vitro models used to screen anti-cancer drugs. "What this 3D lung cancer co-culture model confirmed for us is that tumor cells are phenotypically different in terms of their viability, activity, and morphology when they grow in the presence of fibroblasts. Likewise, the stromal cells associated with tumors also behave differently in co-culture than they do alone, becoming more like myofibroblasts, cells that are known to assist tumor growth, invasion, and metastasis. This model creates an even more native in vitro tumor environment to more easily assess tumor growth, pathobiology, and drug efficacy." 

Using Insphero's automation-compatible, high-throughput platform, the authors plan to conduct advanced screens for improved anti-cancer drugs and to indentify novel NSCLC biomarkers.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
Genes That Protect African Children From Developing Malaria Identified
Variations in DNA at a specific location on the genome that protect African children from developing severe malaria, in some cases nearly halving a child’s chance of developing the life-threatening disease, have been identified in the largest genetic association study of malaria to date.
Researchers Disguise Drugs As Platelets to Target Cancer
Researchers have for the first time developed a technique that coats anticancer drugs in membranes made from a patient’s own platelets.
Dormant Viral Genes May Awaken to Cause ALS
NIH human and mouse study may open an unexplored path for finding treatments.
Scientists Create World’s Largest Catalog of Human Genomic Variation
An international team of scientists from the 1000 Genomes Project Consortium has created the world’s largest catalog of genomic differences among humans, providing researchers with powerful clues to help them establish why some people are susceptible to various diseases.
Five Genetic Regions Implicated In Cystic Fibrosis Severity
An international consortium of researchers conducted the largest-ever CF genome-wide analysis to find new therapeutic targets.
Greater Understanding Of Polycystic Ovary Syndrome
A new genetic study of over 200,000 women reveals the underlying mechanisms of polycystic ovary syndrome, as well as potential interventions.
New Autism Genes Are Revealed in Largest-Ever Study
Work draws more detailed picture of genetic risk, sheds light on sex differences in diagnosis.
A Fundamental Protection Mechanism Against Formalin In Mammals is Revealed
Formaldehyde, or formalin, is well known to all of us as a common chemical used in many industrial processes and also as a preservative, remarkably we also produce formaldehyde in our bodies.
A New Single-Molecule Tool to Observe Enzymes at Work
A team of scientists at the University of Washington and the biotechnology company Illumina have created an innovative tool to directly detect the delicate, single-molecule interactions between DNA and enzymatic proteins.
Genetic Adaptations to Diet and Climate
Researchers found genetic variations in the Inuit of Greenland that reflect adaptations to their specific diet and climate.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos