Volume-Related Inhibitors Standardization for Reverse Transcription Quantitative Polymerase Chain Reaction Experiments
Pascal Pugniere, Sebastien Banzet, Thomas Chaillou, Catherine Mouret and Endre PeinnequinThis poster addresses the reliability of qPCR data and its dependence on technical variations. The proposal is that constant volume of RNA extract in order to improve reliability. |  | |
Defining off-target cleavage in a pair of Zinc Finger Nucleases
K. Mukherjee, D. CarrollThis study looks at off-target cleavage of Zinc Finger Nucleases (ZNFs) in Drosophila in an attempt to analyze potential cleavage spots, with a view to designing more efficient ZFNs. | | |
Gene Expression Profiling: qPCR Toolkit for Quality Control
Švec D., Jacobsson J., Sjöback R., Kubista M.TATAA Biocenter explain how they developedand optimized high-throughput gene expression qPCR with ValidPrime quality control, compensating for inter-run variations. | | |
Cytotoxicity Screen of Mangiferin and its Major Metabolite Norathyriol in Human Tumor Cell Lines
Souza, J.R.R., Feitosa, J.P.A., Ricardo, N.M.P.S, Trevisan, M.T.S., Frei, E., Ulrich C.M., Owen, R.W.Many natural products are available worldwide as potential chemoprotective agents against commonly occurring cancers, for example Mangiferin which has low bioavailability and is thought to be mainly available in the colon. | | |
Polarized Cell Expansion and Heterochronic Shift in Angiosperm Spermatogenesis
Said Hafidh, Katarina Breznenova, Jana Fecikova, Vera Capkova and David HonysThis study evaluates genetic factors and common pathways in polarized cell-tip expansion. | | |
Identification and Characterisation of Disease Resistance Pathway Genes in Jute (Corchorus spp.)
Niaz Mahmood, Salma Sultana Alam, Rifit Ara Najnin, Nurun Nahar Fancy, Haseena Khan.Study aims include identification and characterisation of all NBS containing genes in jute using degenerate primers designed from the conserved NBS domain, by using two species of jute; susceptible and wild type resistant varieties. | | |
Effect of Liquid Handling Quality Control on Biological Assay Performance
Nathaniel Hentz, Ph.D and Tanya KnaideHigh quality assays are required in order to take forward potential drugs, post screening. Therefore the liquid handling must be accurate and precise, as demonstrated in this experiment. | | |
Why Is My Assay Failing? An Approach to Assay Equipment Optimization
Tanya R. Knaide, John Thomas Bradshaw, Kevin Khovananth, Keith AlbertAssays can produce unexpected or failing results for a multitude of reasons. Variability may be introduced at any point within the assay process. | | |
An Automated, Cell-based Platform for the Rapid Detection of Novel Androgen Receptor Modulators
Brad Larson, Bruce Sherf (INDIGO Biosciences), and Peter BanksThe Androgen Receptor (AR) is a member of the family of nuclear receptors responsive to steroid hormones. This poster aims to devise, validate and perform a preliminary automated HTS screening campaign to identify novel modulators of AR activity. | | |
Automated Solutions for Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cellular Cytotoxicity Utility
Brad Larson, Peter Banks , Nicolas Pierre, Stéphane Martinez, and Francois DegorceSince the end of the 1990’s, the pharmaceutical industry has seen an increased interest in biologics, especially in the therapeutic areas of oncology and inflammation. Here we present the automation of two assays for the characterization and selection of potent antibody drug candidates. Both assays rely on HTRF® detection. The first assay quantifies the binding affinity of antibodies to their target antigen, on live cells. | | |
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Evaluation of Candidate Reference Genes for Gene Expression NormalizationStudy performed in Brassica juncea using real time quantitative RT-PCR.
Reliable and Rapid Characterization of Functional FCN2 Gene Variants Reveals Diverse Geographical PatternsIn this study, scientists genotyped and characterized four FCN2 functional SNP variants in West Africans, South Americans, South East Asians from Vietnam and Caucasians from Germany using an available rapid, reliable and cost effective FRET-RTPCR methodology.
Good News for NanomedicineQuantum dots appear safe in pioneering study on primates.
Research on Butterflies Reveals Genetic SharingLandmark effort to sequence the genome of a South American butterfly has revealed the key behind its ability to mimic other butterflies.
Zooming in on Early Embryonic DevelopmentBroad Institute researchers show a high-resolution view of mouse embryogenesis, down to individual bases, showing genetic changes.
New Technique Reveals Unseen Information in DNA CodeScientists have developed and tested a technique to distinguish between two modified forms of cytosine.
NIH Study Finds that Coffee Drinkers have Lower Risk of DeathOlder adults who drank coffee — caffeinated or decaffeinated — had a lower risk of death overall than others who did not drink coffee.
Finnish Researchers Discover Genes Inhibiting the Spread of Prostate CancerResearchers utilize the cell spot micro array technology to study the impacts of all genes in an entire genome in a single experiment.
Stabilizing Fanconi Anemia with AntioxidantsFanconi anemia (FA) is a rare genetic disorder which affects one person in 350,000.
Scientists Make Groundbreaking Discovery Of Mutation Causing Genetic Disorder In HumansScientists at A*STAR's Institute of Medical Biology (IMB), in collaboration with doctors and scientists in Jordan, Turkey, Switzerland and USA, have identified the genetic cause of a birth defect known as Hamamy syndrome.
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