|DNA-free CRISPR-Cas9 genome engineering in zebrafish|
Amanda Haas, Alex J. Blasky*, Rytis Prekeris*, John A. Schiel, Melissa L. Kelley, and Anja van Brabant Smith | Dharmacon, now part of GE Healthcare, 2650 Crescent Drive, Suite 100, Lafayette, CO 80026, USA *University of Colorado - Anschutz Medical Campus, Department of Cell & Developmental Biology, Denver, CO, USA
Poster describing the advantages of a DNA-free gene editing system and the application of this system in zebrafish.
|Analysis of Terpenes Using Gas Chromatography with Vacuum Ultraviolet |
Changling Qiu, Jonathan Smuts, Phillip Walsh, and Kevin A. Schug
The VUV absorption spectra for different terpenes were distinctive and differentiable. GC-VUV demonstrated the capabilities for qualitative and quantitative analysis of terpenes in turpertine mixtures. Chromatographic coeluting signals can be deconvolved by the VUV data analysis software.
|600 base reads on the Ion S5™ Next-Generation Sequencing System enables accurate HLA typing of 96 samples on one 530™ chip|
Peter B. Vander Horn, Cisilya Duncan, Jamsheed Ghadiri, Amneet Gulati, Diana Jeon, April Jung, Mindy Landes, Tommie Lincecum, Geoffrey Lowman, Vadim Mozhayskiy, Linus Ong, Xinzhan Peng, Maryam Shenasa, Prasanna Thwar
We have demonstrated that by combining improvements in templating and sequencing biochemistry we are able to sequence templates longer than 600 bases with high accuracy on an Ion S5 530 chip.
These improvements open the S5 use space to include haplotyping applications that require longer reads. As a demonstration of that, we accurately typed 96 HLA samples on one 530 chip.
|Assessing Diversity in Cassava through the Application of Metabolomics|
Margit Drapala, Elisabete Carvalhoa, Laura Perez-Fonsa, Elliott Pricea, L. Augusto Becerra Lopez-Lavalleb, Paul D. Frasera
In the present study metabolomic platforms have been established for Cassava and used to assess the biodiversity present in Cassava germplasm collections and elucidate underlying biochemical mechanisms associated with traits of interest.
|Metabolic response to everolimus in patient-derived xenografts of triple negative breast cancer|
Leslie R. Euceda1, Deborah K. Hill1,2, Endre Stokke1, Elisabetta Marangoni3, Tone F. Bathen1, Siver A. Moestue1,2
Everolimus treatment metabolic effects on TNBC PDX models were investigated using MR spectroscopy. PLS-DA successfully discriminated treated from controls and PDX expressing INPP4B and not. LMM revealed significant differences in 4/17 metabolites and two metabolite ratios between treated and controls.
|Design considerations for highly specific and efficient synthetic crRNA molecules|
Anja van Brabant Smith, Emily M. Anderson, Shawn McClelland, Elena Maksimova, Tyler Reed, Steve Lenger, Žaklina Strezoska, Hidevaldo Machado Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Suite #100, Lafayette, CO 80026, US
An overview of our rational design algorithm for picking highly functional crRNA sequences in combination with comprehensive specificity analysis.
|A Novel Bioluminescent HTS Method for Rapid NAD(P)/NAD(P)H Detection Poster|
Jolanta Vidugiriene, Donna Leippe, Mary Sobol, Wenhui Zhou, Gediminas Vidugiris, Troy Good, Laurent Bernad, Poncho Meisenheimer and James J. Cali
Nicotinamide adenine dinucleotides (NAD+, NADH, NADP+ and NADPH) are fundamental co-factors of cellular energy metabolism. These dinucleotides are essential for macromolecule biosynthesis and the maintenance of the cellular redox potential. Our new rapid, easy-to-use assays for measuring dinucleotides are convenient tool for investigating their role in these processes.
|Design and Validation of Bioluminescent Assays for 3D Cell Culture Models Poster|
Terry L. Riss, Michael P. Valley, Chad A. Zimprich, Andrew L. Niles, Kevin R. Kupcho and Dan F. Lazar
Cells cultured in 3D model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D monolayer of cells grown on standard plastic plates.
|iPSC-Derived Cardiomyocytes and Luciferase Reporters: A Robust Reporting Platform for Monitoring Cardioprotection and Pathway Biology in Endogenous Human Tissue Cells|
Fiene, S., Thompson, A., Niles, A., Robers, M., Anson, B.
Pathophysiological conditions, medical interventions, and off-target toxicities can all result in cellular oxidative stress. In cardiac myocytes, prolonged and/or excessive oxidative stress can lead to cardiotoxicity: a primary cause of developmental delays, black-box warnings, and post-launch withdrawal of pharmaceuticals.