Corporate Banner
Satellite Banner
Genomics
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Big Genetics in BC: The American Society for Human Genetics 2016 Meeting
Themes at this year's meeting ranged from the verification, validation, and sharing of data, to the translation of laboratory findings into actionable clinical results.
Cancer Genetics: Key to Diagnosis, Therapy
When applied judiciously, cancer genetics directs caregivers to the right drug at the right time, while sparing patients of unnecessary or harmful treatments.
Tissue Damage Is Key for Cell Reprogramming
Researchers have shown tissue damage is important for cells to return to an embryonic state for cell reprogramming.
Improving Drug Production with Computer Model
A model has been developed that can be used to improve and accelerate the production of biotherapeutics, cancer drugs, and vaccines.
New Form of Autism Found
An international team of researchers have identified a new form of syndromic autism.
Accelerating the Detection of Foodborne Bacterial Outbreaks
The speed of diagnosis of foodborne bacterial outbreaks could be improved by a new technique developed by researchers at the Georgia Institute of Technology.
Scientists Identify Unique Genomic Features in Testicular Cancer
The findings may shed light on factors in other cancers that influence their sensitivity to chemotherapy.
Top 10 Life Science Innovations of 2016
2016 has seen the release of some truly innovative products. To help you digest these developments, The Scientist have listed their top picks for the year.
Secret Phenotypes: Disease Devils in Invisible Details
Algorithmic deep phenotyping exposes masses of hidden traits and possible subtle genetic connections relevant to unseen influences on disease.
Hunting the Missing Link Between Genetics and the Environment
The International Phenome Centre Network (IPCN) works to transform healthcare through phenomics - the dynamic interactions between our genes and our environment.
Scroll Up
Scroll Down

BRD4 Bromodomain 1 Inhibitor Screening Using a Homogeneous Proximity Assay
Bookmark and Share

BioTek Instruments

Epigenetics refers to a dynamic set of processes that regulate transcription of the genome, some involving chemical modification of histone proteins. Histones associate with DNA to form nucleosomes, which bundle to form chromatin and chromosomes. Histones contain a flexible N-terminus tail, which protrudes from the nucleosome and is the site for epigenetic modifications. Modifications to amino acid residues in the histone tail include the addition and removal of functional groups, such as methyl and acetyl groups by specific enzymes. Other proteins affect epigenitic changes by reading whether the histone tail has been modified. A bromodomain protein is one such protein that recognizes and binds to the acetylated lysine residues in the histone tail. Inhibition of this protein: protein binding event is of major interest in some forms of cancer. This application highlights an HTS screening assay useful for studying the inhibition of bromodomain/histone binding. Continued interest in histone modifying proteins as druggable targets makes these types of assays an important focus area given the increased performance of the Synergy™ Neo HTS instrument. The assay is an extremely rapid and easy to perform biochemical assay with little optimization required aside from some initial instrumentation optimization. This assay was easily scaled to a 384-well format required for screening of small compound libraries.

Further Information

Related Content

DLReady Certification Awarded for Synergy 2 and Synergy HT Multi-Detection Microplate Readers
Awarded by Promega Corporation, this certification validates an instrument to the highest performance levels and standards for the Dual-Luciferase® Reporter Assay System.
Monday, November 06, 2006
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
4,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,300+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!