Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

CYTOO’s 2D+ Cell Culture Platform Enables Quantitative Mapping

Published: Wednesday, September 19, 2012
Last Updated: Wednesday, September 19, 2012
Bookmark and Share
New applications represent a major step towards quantitative high content analysis of cellular organization in high throughput screening.

CYTOO S.A., has announced new results that demonstrate the ability of the Company’s 2D+ Cell Culture Platform to enable two new high content analysis (HCA) approaches: (i) quantitative density mapping of trafficking compartments and (ii) standardized mitochondrial analysis.

The results will be presented next week at MiPTec 2012 in Basel by the researchers from the Institut Curie in Paris and Angers University Hospital/INSERM, France.

The CYTOO 2D+ Cell Culture Platform, which was launched in July 2012, is based on the use of adhesive micropatterns in microplate wells to guide the cell architecture and behavior in culture.

By defining the 2D topology of cell adhesion, the 2D+ Technology enables the fine control of the spreading and 3D shape of cultured cells in single- or multi-cellular configurations resulting in control of cell contractility, cell polarity, organelle positioning, or cell division axis.

This opens new perspectives for more physiologically relevant cell-based assays compared to classical 2D culture where cells spread and move in an uncontrolled manner.

The researchers of Bruno Goud’s team at the Institut Curie will present at MipTec novel data showing the use of the 2D+ Cell Culture Platform to identify proteins that sustain steady-state organization of trafficking compartments by high-throughput density mapping in several compound and siRNA high content screens.

Following the method developed previously by the team, the hits were selected based on the P-value calculated from the difference between the density map of a pooled control and the density map of each treated condition in the screen.

The data from only 30 cells per condition was sufficient to get statistically significant results.

In comparison, “state-of-the-art” HCA using unrestricted cells with considerable cell-to-cell variation requires hundreds or thousands of cells in order to get significant results.

Arnaud Chevrollier from Angers University Hospital will present the use of the CYTOO 2D+ technology to standardize the quantification of mitochondrial networks in primary skin fibroblasts.

The increasing importance of defective mitochondrial dynamics in several diseases including common neurodegenerative disorders, cardiomyopathies, or age-related disorders, has called for the development of reference imaging tools for medical investigation and drug discovery.

However, until now current approaches to study complex mitochondrial morphology have been limited to low throughput analysis due to the ever varying shape and distribution of the mitochondrial network in cells with heterogeneous size and morphology that is inherent to culture conditions but has no direct biological significance.

The CYTOO 2D+ micropatterned arrays enabled standardization of the size and shape of the cells in culture allowing, for the first time, a high content quantitative relationship between the organization of the mitochondrial network and specific experimental and pathological conditions.

This novel approach can also be applied to high content screening to systematically identify compounds and pathways that impact mitochondrial abundance and sub-cellular distribution.

Commenting on the new results, Michel Bornens, Ph.D, CYTOO Chief Scientific Officer, said: “These two studies to be presented at MiPTec this year demonstrate how, 2D+ Cell Culture Platform enables completely new powerful high content analysis approaches that were previously impossible. We are delighted to see this new data, which represent a major step towards quantitative HCA of cellular organization in high throughput screens.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

CYTOO, Cenix BioScience and Academic Collaborators Receive 4 M€ EU FP7 Grant to Launch MEHTRICS Consortium
New public-private consortium to further develop the power of high throughput, high content RNAi screening through convergence with micropatterning-based cell assays.
Monday, December 12, 2011
CYTOO Raises 10 M$ (7M€) in Series C Round
CYTOO to use the financing to fully exploit its fast growing business.
Tuesday, November 29, 2011
Scientific News
Charting Kidney Cancer Metabolism
Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.
Insights into the Function of the Main Class of Drug Targets
About thirty percent of all medical drugs such as beta-blockers or antidepressants interact with certain types of cell surface proteins called G protein coupled receptors.
Visualizing a Cancer Drug Target at Atomic Resolution
Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.
Honey’s Potential to Save Lives
The healing powers of honey have been known for thousands of years.
3-D Printed Lifelike Liver Tissue for Drug Screening
A team led by engineers at the University of California, San Diego has 3D-printed a tissue that closely mimics the human liver's sophisticated structure and function. The new model could be used for patient-specific drug screening and disease modeling.
Cytoskeleton Crew
Findings confirm sugar's role in helping cancers survive by changing cellular architecture.
Biomarker for Recurring HPV-Linked Oropharyngeal Cancers
A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University.
Valvena, GSK Sign New R&D Collaboration
Valneva to supply process development services for EB66® -based Influenza vaccines.
Light Signals from Living Cells
Fluorescent protein markers delivered under high pressure.
Cellular 'Relief Valve'
A team led by scientists at The Scripps Research Institute (TSRI) has solved a long-standing mystery in cell biology by showing essentially how a key “relief-valve” in cells does its job.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!