Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

CYTOO’s 2D+ Cell Culture Platform Reproduces in vivo Conditions to Study Tumor Cell Motility

Published: Thursday, October 18, 2012
Last Updated: Thursday, October 18, 2012
Bookmark and Share
New perspectives in oncology, genetics and drug screening.

CYTOO S.A. announced new results that demonstrate the ability of the Company’s 2D+ Cell Culture Platform to reproduce in vivo conditions to analyze tumor cell motility and in particular to study fibrillar ECM-dependent tumor cell-macrophage pairing and migration involved in tumor metastasis. These results have recently been published in the first issue of the journal IntraVital, edited by Landes Bioscience.

The CYTOO 2D+ Cell Culture Platform is based on the use of adhesive micropatterns to guide cell architecture and behavior in culture, in contrast to current 2D cell culture where cells spread and move in an uncontrolled manner. By defining the 2D topology of cell adhesion, 2D+ Technology enables the fine control of the spreading and 3D shape of cultured cells in single- or multi-cellular configurations resulting in control of cell contractility, cell polarity, organelle positioning, or cell division axis.

Researchers Ved Sharma, Brian Beaty, Antonia Patsialou, Dianne Cox, John Condeelis and Robert Eddy from the Albert Einstein College of Medicine, NY, with collaborators Huiping Liu from University of Chicago and Michael Clarke at Stamford School of Medicine, used CYTOOchipsTM Motility to reconstitute an in vitro model of fibrillar tumor extracellular matrix (ECM). The micropatterned 1D adhesive tracks were used to mimic the linear ECM fibers of the tumor microenvironment.

Similar morphologies, behaviors and motility rates were observed in vivo and on micropatterned lines. In particular, tumor cell velocity on 1D substrates was in agreement with the high velocity values of tumor cells on ECM fibers observed in vivo. In contrast, on classical 2D substrates, motility rates were ten fold lower than what can be observed in vivo. On micropatterned lines, the authors could also reproduce the assembly of alternating tumor cells and macrophages identified as streams in vivo, the ability of macrophages to enhance protrusion velocity and average velocity of tumor cells and showed that this effect was dependent on an intact paracrine loop without any additional need of co-factors.

The authors concluded that their “1D micropatterned substrate model more closely approximates the fibrillar nature of the in vivo tumor microenvironment and offers a simple and more appropriate substrate for detailed analyses of cell protrusion, cell-cell pairing and migration than conventional 2D substrates. The data presented here validates the use of micropatterned 1D adhesive substrates to study the fibrillar ECM found within the tumor microenvironment.”

Co-author Robert Eddy commented “It was a surprise that tumor cell and macrophage streaming behavior we observe in the highly complex tumor microenvironment was self-organizing and required no other extracellular cues on 1D adhesive substrates.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Olivier Pasquier Joins CYTOO as Chief Commercial Officer
Olivier Pasquier first graduated from the French University Nice-Sophia Antipolis in molecular biology. He then completed his background with a marketing MBA from ESCP Europe.
Monday, March 18, 2013
Scientific News
Charting Kidney Cancer Metabolism
Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.
Insights into the Function of the Main Class of Drug Targets
About thirty percent of all medical drugs such as beta-blockers or antidepressants interact with certain types of cell surface proteins called G protein coupled receptors.
Visualizing a Cancer Drug Target at Atomic Resolution
Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.
Honey’s Potential to Save Lives
The healing powers of honey have been known for thousands of years.
3-D Printed Lifelike Liver Tissue for Drug Screening
A team led by engineers at the University of California, San Diego has 3D-printed a tissue that closely mimics the human liver's sophisticated structure and function. The new model could be used for patient-specific drug screening and disease modeling.
Cytoskeleton Crew
Findings confirm sugar's role in helping cancers survive by changing cellular architecture.
Biomarker for Recurring HPV-Linked Oropharyngeal Cancers
A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University.
Valvena, GSK Sign New R&D Collaboration
Valneva to supply process development services for EB66® -based Influenza vaccines.
Light Signals from Living Cells
Fluorescent protein markers delivered under high pressure.
Cellular 'Relief Valve'
A team led by scientists at The Scripps Research Institute (TSRI) has solved a long-standing mystery in cell biology by showing essentially how a key “relief-valve” in cells does its job.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!