Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
Become a Member | Sign in
Home>News>This Article

PCR-based Pool Screening Method for High Throughput Wuchereria Bancrofti Detection

Published: Wednesday, April 24, 2013
Last Updated: Wednesday, April 24, 2013
Bookmark and Share
The purpose of this study was to improve the DNA extraction protocol and qPCR assay to achieve the detection of a single mf in a pool of at least twelve 60-µl blood samples.

Effective diagnostic tools are necessary to monitor and evaluate interruption of Lymphatic  Filariasis (LF) transmission. Accurate detection of Wuchereria bancrofti (Wb) microfilaria  (mf) is essential to measure the impact of community treatment programmes. PCR-based  assays are specific, highly sensitive tools allowing the detection of Wuchereria bancrofti DNA in human blood samples. However, current protocols describing the pool screening  approach, use samples of less than 60 µl of blood, which limits the sensitivity of the poolscreen PCR assay. The purpose of this study was to improve the pool-screen PCR protocol to  enhance its sensitivity and usefulness for population scale studies.

DNA extractions were performed with the DNeasy kit, the PCR with the Wb LDR primers  and the SYBR-Green dye. Improvements of our pool-screen real-time PCR (qPCR) assay  allowed the detection of as little as one Wb microfilaria diluted in a pool of at least 12 blood  samples of 60 µl each. Using this assay, mf burdens can be predicted using a standard curve  derived from mf spiked dried blood samples. The sensitivity achieved is equivalent to the  detection of a single LF positive individual carrying a mf burden as low as 18 mf/ml, in a  pool of blood samples from at least 12 individuals.

Due to its sensitivity, rapidity and cost-effectiveness, we suggest this qPCR pool-screening  assay could be used as a diagnostic tool for population- scale filariasis elimination monitoring  and evaluation.

This article is published online in the Journal Parasites and Vectors and is free to access.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Advanced Cell Culture Techniques for Cancer Drug Discovery
This review focuses on the culturing of cell lines representative of solid cancers in advanced cell culture conditions.
Thursday, June 12, 2014
Application of MALDI-TOF Mass Spectrometry in Clinical Virology
MS methods can be used currently for viral diagnosis but refinement of technology will be an important approach for the introduction of MALDI-TOF in routine viral diagnosis.
Monday, December 16, 2013
Identification of Plasma Lipid Biomarkers for Prostate Cancer by Lipidomics and Bioinformatics
This study explores a new approach to seeking biomarkers for prostate cancer and other diseases.
Monday, April 08, 2013
A Hybrid Structural Approach to Analyze Ligand Binding by the 5-HT4 Receptor
This study reveals the potential of hybrid structural methods, including mass spectrometry to probe physiological and functional GPCR-ligand interactions with purified native protein.
Friday, February 15, 2013
Simultaneous Saccharification and Co-Fermentation for Bioethanol Production at Lab, PDU and Demo Scales
In this study, researchers designed a SSCF process and scaled up from lab to demo scale reaching 4% (w/v) ethanol using xylose rich corncobs.
Monday, February 04, 2013
Genome Engineering using Targeted Oligonucleotide Libraries and Functional Selection
Exploiting Red-mediated recombineering to perform scanning mutagenesis using synthetic oligonucleotide libraries.
Wednesday, June 06, 2012
Binding-site Assessment by Virtual Fragment Screening
This article reports the results of virtually screening ~11,000 diverse fragment-like compounds against a total of 152 protein binding sites.
Wednesday, June 06, 2012
FTMAP: Extended Protein Mapping with User-selected Probe Molecules
A direct computational analogue that can be used to sample the surface of a target protein using small organic molecules as probes.
Wednesday, May 23, 2012
A Comparative Study of Fragment Screening Methods
New methods and insights into fragment screening on the p38a kinase.
Tuesday, May 22, 2012
Specificity Quantification of Biomolecular Recognition and its Implication for Drug Discovery
Highly efficient and specific biomolecular recognition requires both affinity and specificity. Here scientists developed a novel method SPA (SPecificity and Affinity) based on funneled energy landscape theory, with increased specificity on previous models.
Monday, March 26, 2012
Structural Studies of Specific Intermolecular Interactions and Self-Aggregation of Biomolecules and Their Application to Drug Design
The group of scientists performed structural analyses on biologically important molecules and their complexes with partners, hoping to contribute to molecular design for controlling biologically abnormal functions.
Monday, March 26, 2012
Core Site-Moiety Maps Reveal Inhibitors and Binding Mechanisms of Orthologous Proteins by Screening Compound Libraries
Study reveals the CoreSiMMap is useful to identify adaptive SK inhibitors and provide insight into the binding mechanisms of compounds.
Friday, March 09, 2012
A High-Throughput Screen Identifies a New Natural Product with Broad-Spectrum Antibacterial Activity
Researchers from Children's Hospital, Boston report the development of a sensitive and robust but low-tech and inexpensive high-throughput metabolic screen for novel antibiotics.
Wednesday, March 07, 2012
Esophageal Cancer Metabolite Biomarkers Detected by LC-MS and NMR Methods
Metabolic profiles derived from the combination of LC-MS and NMR methods were shown to readily distinguish EAC patients and potentially promise important routes to understanding the carcinogenesis and detecting the cancer.
Tuesday, March 06, 2012
The Benefits of Constructing Leads from Fragment Hits
Since it is now clear that fragments can be developed into clinical candidates, the discussion is shifting to the design of good-quality lead compounds from fragment hits.
Monday, February 27, 2012
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
Measuring microRNAs in Blood to Speed Cancer Detection
A simple, ultrasensitive microRNA sensor holds promise for the design of new diagnostic strategies and, potentially, for the prognosis and treatment of pancreatic and other cancers.
Potential Persistent Tuberculosis Treatment
Researchers have discovered several first-in-class compounds that target hidden TB infections by attacking a critical process the bacteria use to survive in the hostile environment of the lungs.
Metabolic Profiles Distinguish Early Stage Ovarian Cancer with Unprecedented Accuracy
Studying blood serum compounds of different molecular weights has led scientists to a set of biomarkers that may enable development of a highly accurate screening test for early-stage ovarian cancer.
The Do’s and Don’ts of SPR Experiments
Surface Plasmon Resonance (SPR) is a technique that is becoming more widely used, particularly by anyone who wants to obtain accurate on (association) and off (dissociation) rates for biomolecular binding.
Long-Sought Protein Sensor for the ‘Sixth Sense’ Discovered
In a study led by scientists from The Scripps Research Institute (TSRI)the sensor protein for propioception has been identified.
New Anti-Malarial Drug Screening Model
University of South Florida researchers demonstrate novel chemogenomic profiling to identify drug targets for the most lethal strain of malaria.
Shedding Light on “Dark” Cellular Receptors
UNC and UCSF labs create a new research tool to find homes for two orphan cell-surface receptors, a crucial step toward finding better therapeutics and causes of drug side effects.
New, Better Test for Prostate Cancer
A study from Karolinska Institutet shows that a new test for prostate cancer is better at detecting aggressive cancer than PSA.
Giant Molecules Inhibit Ebola Infection
European researchers have designed a "giant" molecule formed by thirteen fullerenes covered by carbohydrates which, by blocking this receptor, are able to inhibit the cell infection by an artificial ebola virus model.

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos