Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Merck Millipore Launches MILLIPLEX® MAP Kits

Published: Tuesday, May 07, 2013
Last Updated: Tuesday, May 07, 2013
Bookmark and Share
Enables detection of multiple pluripotency markers in a single measurement.

Merck Millipore has announced availability of the MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kits, which enable the analysis of many different pluripotency biomarkers in a single measurement.

These kits are the first commercially available multiplexed protein biomarker assays based on the Luminex® xMAP® platform for this important research area.

This new application of trusted bead-based multiplex technology gives researchers rapid feedback on the potency status of stem cell cultures and offers a significant advantage over existing techniques, which detect a limited number of proteins with a semi-quantitative output for a single sample.

Merck Millipore's new MILLIPLEX® MAP kits examine protein biomarkers that indicate the potency of stem cells, enabling pluripotent stem cells to be distinguished from multipotent or differentiating cells.

These new kits save time and resources by accurately detecting large numbers of pluripotency markers in one measurement. Kits can be used on cultures of induced pluripotent stem cells, human embryonic stem cells, cancer stem cells or cancer cell lines.

Two kits are available for assessing stem cell pluripotency. The MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kit 1 monitors the expression of the transcription factors Oct 3/4, Sox2, Nanog and c-Myc, which play critical roles in the maintenance of pluripotency and self-renewal in stem cells.

The MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kit 2 contains seven additional pluripotency-related markers, including a transcription factor, a translational regulator, transmembrane proteins and cell surface glycostructures.

These assays are essential for assessing the potency of stem cell cultures in studies ranging from cancer and developmental biology research to bioprocess monitoring. MILLIPLEX® MAP assays make it easy to analyze both cell surface and intracellular proteins.

"The MILLIPLEX® MAP Stem Cell Pluripotency Kit 1 is a valuable tool for stem cell research. We obtained very interesting and promising results," said Frank Edenhofer, Ph.D., Head of the Stem Cell Engineering Group, Institute of Reconstructive Neurobiology, University of Bonn, LIFE & BRAIN Center. "It is noteworthy that this assay enabled us to observe even early onset of differentiation in iPS cell cultures."

"Stem cells are of great interest for regenerative medicine and drug discovery efforts, and researchers must be able to effectively characterize the potency status of pluripotent stem cells to advance their progress toward clinical use," said Linda Meeh, Ph.D., Director of Marketing for Immunoassays and Multiplexing.

Meeh continued, "MILLIPLEX® MAP kits offer a quick and simple way to achieve this characterization, generating a comprehensive assessment of the overall quality of cell cultures."


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Merck Millipore, VWR Extend Western European Distribution Agreement
Provides VWR access to more Merck Millipore products and reagents for chemical and microbiological analysis.
Wednesday, April 09, 2014
Merck Millipore Announces New Genomic Biomarker Services
Genetic and epigenetic technologies added to established CRO services for biomarker development, validation and testing.
Wednesday, December 05, 2012
Scientific News
Mass Spec Technology Drives Innovation Across the Biopharma Workflow
With greater resolving power, analytical speed, and accuracy, new mass spectrometry technology and techniques are infiltrating the biopharmaceuticals workflow.
One Step Closer to Precision Medicine for Chronic Lung Disease Sufferers
A study led by University of North Carolina at Chapel Hill, and National Jewish Health, has provided evidence of links between SNPs and known COPD blood protein biomarkers.
Peer Reviewed Study Demonstrates Mass Spec Technique
The peer reviewed study demonstrates MS workflow, TMTCalibrator workflow, which dramatically enhances detection of key early stage Alzheimer’s biomarkers.
Small Molecules Lead to Big Change in Reaction Outcomes
Scientists have changed the behaviour of a group of molecules involved in carbon-oxygen bond synthesis.
Enhancing Antibiotics to Defeat Resistant Bacteria
Scientists enhance ability of antibiotics to defeat resistant types of bacteria using molecules called PPMOs
Sanger Institute, St Jude Data-Sharing Agreement
Childhood cancer targeted by Sanger Institute and St Jude Children’s Research Hospital exchanges of cancer data
Over Two-Thirds of Cervical Cancer Deaths Prevented
Cervical screening prevents 70% of cervical cancer deaths and if all eligible women regularly attended screening this would rise to 83%.
Space Research Fighting Cancer
JPL and National Cancer Institute renew Big Data partnership that 'learns' data similarities.
Uncovering the Genetics Behind High Blood Pressure
Results suggest a role for blood vessels themselves in controlling blood pressure.
Detecting Bacterial Infections in Newborns
Researchers tested an alternative way to diagnose bacterial infections in infants—by analyzing RNA biosignatures from a small blood sample.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!