Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
Become a Member | Sign in
Home>News>This Article

Redd&Whyte Preddator System for DMSO Back-filling Used by AstraZeneca

Published: Tuesday, September 03, 2013
Last Updated: Tuesday, September 03, 2013
Bookmark and Share
Application increases throughput by a third and improves assay accuracy.

Redd&Whyte has announced that its Preddator system has been integrated in an assay workcell by AstraZeneca, to meet a generic high throughput nano-litre dispensing application.

Using the Preddator system, the assay run time has been significantly reduced, while maintaining the dispensing consistency and flexibility of the original workcell.

Scientists in the Oncology Group at AstraZeneca (Alderley Park, UK) integrated the Preddator system as an additional dispensing module to complement a Labcyte ECHO 555 acoustic reformatter in a high throughput cell-based screening workcell.

The system is routinely used to perform 2-dimensional dose response dispensing for drug combination screening. It uses the nano-litre dispensing platform across over 250 plates in 384-well format to DMSO backfill volumes ranging between 20 and 100nL over ~36 hrs per batch in each run.

The main function of the Preddator system is to carry out the DMSO backfill step to maintain a constant level of DMSO across all assay wells.

This is important due to the nature of DMSO-sensitivity of cell-based assays being carried out and the effects that varying levels of DMSO may have on the growth of some cell lines.

John Vincent, Senior Research Scientist in Oncology at AstraZeneca commented: “It can take us around 36 hours to run an assay. Since DMSO is very hygroscopic and absorbs water quickly, this can change the concentration of the compound being screened. This can affect the accuracy of compound transfer by the Echo reformatter if the compound source plate is progressively hydrated and utilized by the system over an extended period. We knew if we could speed up the work-flow by having an efficient nano-litre dispenser to carry out the DMSO back-filling separately, we could improve the consistency of our compound dosing. This would make a significant saving of stock compounds as a result of requiring fewer stock plates and a shorter overall run time. The Preddator allows us to do this.”

Vincent continued: "We chose the Preddator system because it is able to dispense nano-litre volumes of different liquids and it has a small footprint which is a critical factor to fit into our bespoke automation workcell. The close collaborative effort between “Redd & Whyte” and PAA has been fundamental in successfully integrating the Preddator into our workcell under the Overlord3 robotic environment. The Preddator is being used as the basic workflow to provide DMSO back-fill after initial compound dosing by the ECHO reformatter. The new workflow with the Preddator is allowing us to reduce our compound dosing step by up to 30 percent. We believe that the Preddator is an effective nano-litre dispensing device which offers high speed, accurate dispensing in a very effective small footprint to complement the existing acoustic reformatter function.”

Roger Poole, Managing Director of Redd&Whyte said: "Dilutions can cause biological compounds to come out of solution so it is critical when preparing activity curves to keep DMSO at an optimum concentration. Keeping DMSO concentration at less than 0.1 percent of assay volume is difficult due to the lack of reliable Nanolitre-range liquid handlers.”

Poole added: “This is why we’re delighted that our Preddator system with its flexible range of precise low volume dosing is showing such good performance at AstraZeneca. Their results demonstrate to scientists that want to save time and improve assay accuracy when running screens that they should look at integrating a Preddator low-volume microplate dispenser into their workflow.”

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Preddator Low-volume Microplate Dispenser Awarded ELRIG Prize
Recognizing technology’s novel method of improving liquid handling efficiency.
Wednesday, July 10, 2013
Redd&Whyte Appoints HighRes Biosolutions as New US Distributor
Appointment offers US scientists specialist access to its unique liquid handling technology.
Thursday, February 28, 2013
Redd&Whyte Appoints New UK Sales Manager
Appointment to provide professional service and support for UK scientists.
Thursday, February 21, 2013
Redd&Whyte Appoints New Director of Business Development
Appointment of Rob Harkness as part of new growth strategy.
Thursday, August 23, 2012
Redd&Whyte wins Best New Company at the Life Science Awards
Redd&Whyte was awarded Best New Company at the inaugural Life Science Awards, on 29 June in Hamburg, Germany, preceding the first European Lab Automation conference and exhibition.
Thursday, July 07, 2011
Scientific News
Potential Target for Treatment of Autism
Grant of $2.4 million will support further research.
Sniffing Out Cancer
Scientists have been exploring new ways to “smell” signs of cancer by analyzing what’s in patients’ breath.
Inroads Against Leukemia
Potential for halting disease in molecule isolated from sea sponges.
Molecular ‘Kiss Of Death’ Flags Pathogens For Destruction
Researchers have discovered that our bodies mark pathogen-containing vacuoles for destruction by using a molecule called ubiquitin, commonly known as the "kiss of death."
A New Single-Molecule Tool to Observe Enzymes at Work
A team of scientists at the University of Washington and the biotechnology company Illumina have created an innovative tool to directly detect the delicate, single-molecule interactions between DNA and enzymatic proteins.
Milestone Single-Biomolecule Imaging Technique May Advance Drug Design
The first nanometer resolved image of individual tobacco mosaic virions shows the potential of low-energy electron holography for imaging biomolecules at a single particle level; a milestone in structural biology and a potential new tool for drug design.
Multi-Gene Test Enables Some Breast Cancer Patients to Safely Avoid Chemotherapy
A major study is providing the best evidence to date that a 21-gene test done on the tumor can identify breast cancer patients who can safely avoid chemotherapy.
Antidepressants Plus Blood-Thinners Slow Down Brain Cancer
EPFL scientists have found that combining antidepressants with anticoagulants slows down brain tumors (gliomas) in mice.
Diagnostics Breakthrough Brings Viral Sequencing to Doctors’ Toolkit
New screening tool produces up to 10,000-fold improvement in viral matches compared with traditional high-throughput methods.
New Virus Identified In Blood Supply
Scientists have discovered a new virus that can be transmitted through the blood supply.

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos