Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Ultra Low Adhesion Plates for Superior 3D Spheroid Cell Culture

Published: Tuesday, March 04, 2014
Last Updated: Tuesday, March 04, 2014
Bookmark and Share
Lipidure®-Coat low adhesion plates and dishes from AMSBIO.

Available from AMSBIO -Lipidure®-Coat low adhesion plates and dishes are shown in cited research to be one of the most effective tools for 3D spheroid cell culture. These new plates and dishes are available in 384-well format optimized for drug screening or other high throughput applications.

Spheroid cell culture is based on the spontaneous formation of an aggregation of cells in an environment where the interactions between cells are superior to the interactions between cells and substrate. This can be achieved by using low-adhesion cell culture conditions.

Lipidure®-Coat surfaces use MPC (2-methacryloyloxtethyl phosphorylcholine), a biocompatible synthetic polymer with the same structure as the phosphorylcholine polar bases that form cell membranes.

As a consequence of the high biocompatibility of this coating, cells will not adhere to the surface of Lipidure®-Coat plates or dishes. Different well-shapes available for 96-well plates (U, V and flat-bottom) can be used to control size, position and density of spheroids.

Studies have shown that Lipidure®-Coat promotes the formation of a single spheroid in each well of U-bottom multi-well plate, while other brands of plate lead to formation of satellite spheroids - indicating a higher degree of cell adhesion is taking place.

Formation of spheroids, tumorspheres, embryonic bodies and neurospheres using LIPIDURE® plates has already been demonstrated for cell types including ES and iPS (human and mouse), NIH3T3, pre-adipocytes, HepG2 and other cancer cell lines as well as primary neuronal cells.

For larger scale assays as well as in applications such as Western blotting or gene expression microarrays - Lipidure®-COAT dishes have produced highly reproducible, top quality results.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Ready-to-Use Tagged cDNA Clones
Available from AMSBIO TrueORF®cDNA clones are tagged cDNA clones for protein studies.
Monday, June 01, 2015
AMSBIO Publishes Comprehensive Cell Culture Handbook
Extensive 48-page handbook is intended for research scientists looking to culture cells in more physiologically relevant environments.
Friday, April 04, 2014
AMSBIO & Sanguine Biosciences Announce Distribution Agreement
Agreement to distribute and support AMSBIO’s products and services throughout Europe.
Friday, August 23, 2013
Scientific News
Liquid Biopsies: Utilization of Circulating Biomarkers for Minimally Invasive Diagnostics Development
Market Trends in Biofluid-based Liquid Biopsies: Deploying Circulating Biomarkers in the Clinic. Enal Razvi, Ph.D., Managing Director, Select Biosciences, Inc.
Crystal Clear Images Uncover Secrets of Hormone Receptors
NIH researchers gain better understanding of how neuropeptide hormones trigger chemical reactions in cells.
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
Sweet Revenge Against Superbugs
A special type of synthetic sugar could be the latest weapon in the fight against superbugs.
Access Denied: Leukemia Thwarted by Cutting Off Link to Environmental Support
A new study reveals a protein’s critical – and previously unknown -- role in the development and progression of acute myeloid leukemia (AML), a fast-growing and extremely difficult-to-treat blood cancer.
Long-sought Discovery Fills in Missing Details of Cell 'Switchboard'
A biomedical breakthrough reveals never-before-seen details of the human body’s cellular switchboard that regulates sensory and hormonal responses.
Tracking Breast Cancer Before it Grows
A team of scientists led by University of Saskatchewan researcher Saroj Kumar is using cutting-edge Canadian Light Source techniques to screen and treat breast cancer at its earliest changes.
Zebrafish Reveal Drugs that may Improve Bone Marrow Transplant
Compounds boost stem cell engraftment; could allow more matches for patients with cancer and blood diseases.
DNA Damage Seen in Patients Undergoing CT Scanning
Along with the burgeoning use of advanced medical imaging tests over the past decade have come rising public health concerns about possible links between low-dose radiation and cancer.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!