Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Easy-to-use, Quantitative Gamma-H2AX Assay

Published: Tuesday, September 02, 2014
Last Updated: Tuesday, September 02, 2014
Bookmark and Share
Gamma H2AX pharmacodynamic assay kit for the study of double strand DNA breaks.

AMSBIO has announced the first commercially available gamma H2AX Pharmacodynamic assay kit for the study of double strand DNA breaks through the detection of gamma H2AX - a phosphorylated histone historically proven as a highly specific and sensitive molecular marker for double strand DNA damage detection.

This new assay has been developed for anti-cancer drug screening, basic research and upcoming clinical trials providing one of many needed tools to support hypothesis-driven drug design strategies.

Documented variability in DNA double-strand break repair among different segments of the human population may contribute to patient specific therapeutic toxicities, enabling a more personalized approach to treatment.

A quantitative determination of gamma-H2AX levels in response to treatment would help to establish patient specific dose regimens minimizing the toxicity, while maximizing the efficacy of therapy.

Until now, induction of gamma-H2AX has been determined by either Western Blots or scoring foci with Immunofluorescence. While Western Blots provide information regarding the gamma-H2AX protein, they are difficult to quantitate and provide mostly qualitative data.

Although image analysis software enables foci quantification, actual gamma-H2AX levels cannot be determined by these techniques, as there is no direct relationship between foci and gamma-H2AX levels after damage.

AMSBIO's new 96-well non-radioactive ELISA assay documents differences in gamma H2AX levels in peripheral blood mononuclear cells, cultured cells, and tissue biopsies and is available as a complete reagent kit with chemiluminescent detection.

Alexei Degretev from Tufts University (Medford, USA), one of the initial beta testers of the product, commented "This new gamma H2AX pharmacodynamic assay is a very useful tool for providing precise quantitation of gamma H2AX formation. The assay is easy to perform, fast, and displays good linear range and excellent reproducibility between replicate samples".


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Ready-to-Use Tagged cDNA Clones
Available from AMSBIO TrueORF®cDNA clones are tagged cDNA clones for protein studies.
Monday, June 01, 2015
AMSBIO Publishes Comprehensive Cell Culture Handbook
Extensive 48-page handbook is intended for research scientists looking to culture cells in more physiologically relevant environments.
Friday, April 04, 2014
AMSBIO & Sanguine Biosciences Announce Distribution Agreement
Agreement to distribute and support AMSBIO’s products and services throughout Europe.
Friday, August 23, 2013
Scientific News
A New Platform for Discovering Antibiotics
Harvard chemists hope to shorten time, difficulty in measuring their effectiveness, potential.
The Need for Speed
Evaluating MALDI-TOF as a high-throughput screening technology for the pharmaceutical industry.
Antarctic Sponge Extract Kills MRSA
New findings may provide opportunity for developing new drugs to fight dangerous bacteria currently highly resistant to treatment.
US-India Collab Finds Molecular Signatures of Severe Malaria
Study may be a significant advancement in understanding the causes of severe malaria.
Novel Way to Prevent Deadly Bacterial Infections
Monash scientists may have found a way to stop deadly bacteria from infecting patients. The discovery could lead to a whole new way of treating antibiotic-resistant “superbugs”
Gene Expression Controls Revealed
Researchers have modelled every atom in a key part of the process for switching on genes, revealing a whole new area for potential drug targets.
An Old-New Weapon Against Emerging Chikungunya Virus
Researchers utilize existing drugs to interfere with host factors required for replication of Chikungunya virus.
Using Gene-editing Technology for Faster, Cheaper Antiviral Drug Development
UCLA scientists are working to develop special screening libraries based on a gene-editing technology called CRISPR.
Can Gender Play A Role In Determining Cancer Treatment Choices?
MD Anderson study reveals “sex-biased” gene signatures in review of 13 cancer types.
New Autism Blood Biomarker Identified
Researchers at UT Southwestern Medical Center have identified a blood biomarker that may aid in earlier diagnosis of children with autism spectrum disorder, or ASD.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!