|Analysis of Terpenes Using Gas Chromatography with Vacuum Ultraviolet |
Changling Qiu, Jonathan Smuts, Phillip Walsh, and Kevin A. Schug
The VUV absorption spectra for different terpenes were distinctive and differentiable. GC-VUV demonstrated the capabilities for qualitative and quantitative analysis of terpenes in turpertine mixtures. Chromatographic coeluting signals can be deconvolved by the VUV data analysis software.
|Mobility of Aeroallergens in Home: Effect of Location of Air Sampling and Implication for Evaluation of Patient Exposure|
Julian Gordon1, Paul Detjen, Andrea Wachter & Prasanthi Gandhi1
The Inspirotec sampler permitted the easy testing of multiple locations within a household. Air sampling simultaneously at 12 locations by other technologies would have been technically challenging. These were run by an untrained operator.
|Selective Debenzylation of N-Benzyloxypyrazinones in Flow|
Anh Hung MAI - Cedrick VERYSER - Wim DE BORGGRAEVE
Selective and reproducible debenzylation of benzyloxypyrazinones by using catalytic transfer hydrogenation in flow chemistry to yield N-hydroxypyrazinones. The flow methodology enabled us to avoid overreduction of the compounds to pyrazin-2(1H)-ones.
|Addressing False Positive Variants Arising from Pseudogenes|
Risha Govind1,2, Sam Wilkinson1,3, Nicola Whiffin1,2, Shibu John1,2, Rachel J. Buchan1,2, Elizabeth Edwards1,2, Deborah J. Morris-Rosendahl1,3, James S. Ware1,2, P.J. Barton1,2, Stuart A. Cook1,2
Clinical genetic testing has been transformed in recent years by the introduction of Next-Generation Sequencing (NGS).
|The Challenges of Genetic Testing in Patients Diagnosed with Breast Cancer; The Kent Oncology Centre Experience|
Christos Mikropoulos1,2, Aaron Davies 1, Charlotte Abson1, Gill Sadler1, Gemma McCormick1, Questa Karlsson2, Julia Hall2
In this study we explore retrospective data to determine strategies for optimizing the genetic referral pathways for breast cancer.
|The Role of microRNAs in Memory Consolidation in Lymnaea|
György Kemenes1, Dimitris Vavoulis2, Sergei Korneev1
In this study we investigated the temporal dynamics of the post-training expression of miRNAs in the ‘learning ganglia’ of Lymnaea.
|Design considerations for highly specific and efficient synthetic crRNA molecules|
Anja van Brabant Smith, Emily M. Anderson, Shawn McClelland, Elena Maksimova, Tyler Reed, Steve Lenger, Žaklina Strezoska, Hidevaldo Machado Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Suite #100, Lafayette, CO 80026, US
An overview of our rational design algorithm for picking highly functional crRNA sequences in combination with comprehensive specificity analysis.
|A New Dual Luciferase Assay Using NanoLuc® Enables a Second Generation Coincidence Reporter System to Reduce False Hits in HTS Poster|
Christopher Eggers, Samuel Hasson, Brock Binkowski, Matt Robers, James Unch, Braeden Butler, , Keith Wood, James Inglese and Frank Fan
Luciferase-based reporter-gene assays remain a cornerstone of high-throughput screening of compounds because of their high sensitivity and dynamic range. However, a substantial number of non-relevant hits can be generated due to direct interaction of compounds with the luciferase reporter.
|CellTiter-Glo® 2.0: A Novel Luminescent Cell Viability Assay with Greatly Enhanced Storage Stability|
Michael P. Valley, James Unch, Poncho L. Meisenheimer, James J. Cali, and Dan F. Lazar
Here we report on the attributes of a novel ATP detection reagent for cell viability with all of the assay performance of the previous CellTiter-Glo® Reagent, but now with markedly enhanced stability as a single component in a liquid format. These new features provide for much greater ease-of-use in that storage of the reagent at 4°C eliminates the requirement for reagent thawing and minimizes temperature equilibration time.