|High Titre BacMam viruses improve transduction efficiency of mammalian cells|
Robert Possee, Elisabetta Locanto, Adam Chambers, Linda King
We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.
|Novel Approaches to High-throughput Measurements of Replicative Lifespan in Yeast by Microfluidic Size Sorting and Genetic Engineering|
Ilka Lewrenz, Stephanie Wälter, Jacqueline Franke
Development of a high-throughput screen utilizing a microfluidic device and a linearly growing yeast strain to simultaneously test the influence of various drugs on the replicative lifespan of yeast.
|Automated Fluorescence Detection and Imaging of RNA Species in Live Cells|
Paul Held, Victor Koong, Don Weldon, Peter Banks
This poster describes the detection and quantification of RNA species in Live cells through automated imaging.
|Identification and Characterisation of Novel Positive Allosteric Modulators of the Galanin 2 Receptor|
An HTS against GalR2 was carried out to find novel PAMs to treat neuropathic pain.
|The Longest Way Round is the Shortest Way Home: HTS Assay Development for Complex Multi-domain Protein Kinases|
Doris Hafenbrandl, Vanessa Nardese, Maria Cristina Sidoli, Valeria Wanke, Mariantonietta Rubino and Daniele Carettoni
This study strongly supports the possibility to overcome the major bottlenecks in the production of long kinase recombinant forms, in order to perform drug discovery programmes with proteins more closely preserving the structural and functional properties of the native enzymes.
|Enabling Epigenetics Studies from HTS to SAR : A Novel HTRF® Platform to Identify and Characterize Reader Domain Inhibitors|
T. Roux1, M. Badol1, N. Douayry1, L. Sergeant1, E.Trinquet1, F. Degorce1, S. Milhas2, S. Betzi2, C. Derviaux2, C. Eydoux3, J. Letienne2, A. Lugari2, Y. Collette2, J-C. Guillemot3 et X. Morelli2
Discover a novel HTRF platform to identify and characterize the vast variety of epigenetic binding domain.
|Artificial Multi-Gene Expression Systems Design Service for Natural Compound Formation and Hetero Protein Complexes|
Bernauer, Hubert, Gregor Zipf and Josef Maier
Drug discovery of natural compounds drug development and drug target analyses as well as bioproduction can benefit from artificial genetic systems and constructions. The direction in which genes are to be developed is written in the genomes. Synthesis oriented genomic analyses of codon bias and tRNA adaption analyses are prerequisites for generating adaptive, highly functional genes.
|Validation of a 3-Dimensional Human Liver Microtissue Model for Long-term Hepatotoxicity Studies|
Brad Larson1, Stewart Hunt2, Timothy Moeller3, Diana Long4, and Peter Banks1
Non-steroidal anti-inflammatory drugs (NSAIDs) are a class of drug commonly used as analgesics and antipyretics, as well as for management of rheumatological disorders. They are one of the most highly prescribed drug families around the world, and consequently, along with antimicrobial agents, are the most frequent causes of druginduced liver injury (DILI) (Bjornsson et al., 2010).
|Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection|
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn
We report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, with a cage surface consisting of positively charged amino groups, which is expected to bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y) exhibits enhanced targeting of U-251 GBM cell lines.