A Mix-and-Read Cell-Based Assay for Antibody Screening Against Epidermal Growth Factor Receptor

The conventional antibody screening assay based on antibody-antigen binding has been enzyme-linked immunosorbent assay (ELISA). While tedious and consuming, ELISA has proved sufficient for the identification of antibodies directed against secreted antigens. However, cell surface antigens (e.g. GPCRs) provide challenges for ELISA due to the shortage of soluble antigens and high variability resulting from loss of cells during wash procedures. Mix-and-read assays (FMAT® assays) overcome such problems.