Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


SoPRano™: High Throughput and Sensitive Label-free Screening on a Plate Reader

Product Description

SoPRano™ is unique in enabling label-free SPR screening in solution using a standard absorbance plate reader, delivering rapid, cost effective, label-free assays for a broad range of applications:

•    Small molecule screening (including fragment screening);
•    Antibody screening and characterisation;
•    ADME and physico-chemical property determination.

particle+image.jpgSoPRano works by using specifically functionalized gold nanoparticles in solution (e.g. coated with a target receptor or antibody) which exhibit Localized Surface Plasmon Resonance (LSPR), a variant of the proven SPR phenomenon. With LSPR, plasmons remain localized on individual nanoparticles and induce an absorbance band in the visible spectrum that shifts with the refractive index of the medium.  This shift is readily quantifiable, such that when ligands interact with the functionalized nanoparticles they cause a reproducible absorbance change in the visible spectrum. As a result, SoPRano technology enables label-free screening on a standard absorbance plate reader.

SoPRano has many advantages that make it ideal for compound, fragment and antibody screening, as well as for high throughput ADME applications:

•    Solution-based ‘mix-and-read’ assays
•    96- and 384-well compatible
•    High sensitivity
•    Reproducible
•    Potential for high throughput
•    Requires only standard lab equipment – no expensive SPR instrument required!
•    Cost effective

In summary, SoPRano™ is a label-free, high throughput and highly flexible early drug discovery technology, that is broadly enabling, cost effective and requires no capital investment.

Build-your-own assay kits
SoPRano ‘build-your-own’ assay kits for small molecule and antibody applications are available, based upon a versatile and easy to use charge adsorption conjugation process:

•    CA-1 and CA-2 charge adsorption kits are available in 20, 100 and 500 ml sizes.

Additional coupling technologies are currently being developed.

The kits include colloidal gold nanoparticles and detailed protocols for conjugation and assay development, enabling you to couple your protein target or antibody and perform screens in your own labs. Kits are available in various sizes, from Starter Kits for preliminary assay development, through to large volume kits for higher throughput applications.

ADME and physico-chemical property kits
ADME and physic-chemical property kits are available for:
•    Human Serum Albumin (HSA)
•    Alpha-1-acid Glycoprotein (AGP)
•    HSA and AGP combination kit, with calculation of Fraction Unbound (Fu)
•    pKa
•    Redox Potential
•    Bioavailablity
•    Blood-Brain-Barrier

The ready to use ADME kits include pre-conjugated gold nanoparticles and detailed protocols.

Contract research services
Our highly experienced team also provides bespoke services for:
•    Assay development;
•    Screening;
•    ADME/physico-chemical assays

Product SoPRano™: High Throughput and Sensitive Label-free Screening on a Plate Reader
Company Pharma Diagnostics
Price Request a quote
More Information View company product page
Catalog Number Unspecified
Quantity Unspecified
Company Logo

Pharma Diagnostics
I&I Center Brussels Z.1 Researchpark 310 1731 Zellik Belgium



Email: enquiries@pharmadiagnostics.com



Scientific News
Potential “Good Fat” Biomarker
New method to measure the activity of energy consuming brown fat cells could ease the testing weight loss drugs.
MicroRNA Pathway Could Lead to New Avenues for Leukemia Treatment
Cancer researchers at the University of Cincinnati have found a particular signaling route in microRNA (miR-22) that could lead to targets for acute myeloid leukemia, the most common type of fast-growing cancer of the blood and bone marrow.
Soy Shows Promise as Natural Anti-Microbial Agent
Soy isoflavones and peptides may inhibit the growth of microbial pathogens that cause food-borne illnesses, according to a new study from University of Guelph researchers.
Doubling Down on Dengue
HMS researchers have discovered two ways a compound blocks dengue virus.
Soy Shows Promise as Natural Anti-Microbial Agent
Researchers from University of Guelph show that soy isoflavones and peptides could be used to reduce microbial contamination of food.
AstraZeneca to Sequence 2 Million Genomes in Search for New Drugs
Company launches integrated genomics approach which aims to transform drug discovery and development.
Unique Model for Studying ALS
Unique mouse model will allow researchers to better study the genetic origins and potential treatments of ALS.
Targeting an ‘Undruggable’ Cancer Gene
RAS genes are mutated in more than 30 percent of human cancers and represent one of the most sought-after cancer targets for drug developers.
Biomarkers for Profiling Prostate Cancer Patients
Exiqon A/S has announced the publication of validation of prognostic microRNA biomarkers for the aggressiveness of prostate cancer in independent cohorts.
Improving Engineered T-Cell Cancer Treatment
Purdue University researchers may have figured out a way to call off a cancer cell assassin that sometimes goes rogue and assign it a larger tumor-specific "hit list."

SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,400+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!