The MaxDiscovery™ Human IL-3 ELISA Test Kit is designed for quantitative determination of the concentration of human IL-3 in serum, plasma, and cell culture supernatant. Human interleukin 3 (IL-3) is a glycoprotein of 133 amino acids after cleavage of a 19 amino acid signal sequence. It is a pleiotropic factor that can stimulate the proliferation, differentiation, and survival of pluripotent hematopoietic stem cells as well as various lineage-committed progenitors. IL-3 is produced by several cell types including activated human T cells, natural killer cells, mast cells, epithelial cells, stromal cells, keratinocytes, neuronsand astrocytes. At the amino acid level, there is only 29% homology between mouse and human IL-3. Consistent with this lack of homology, the proteins are species-specific in their actions. IL-3 exerts its biological activities through binding to heteromeric transmembrane receptors. It has been shown that IL-3 alone, or acting synergistically with other early acting hematopoietic growth factors including, IL-1, IL-6, stem cell factor, G-CSF, MGDF, and Flt-3 ligand, can support the proliferation of pluripotent hematopoietic stem cells and progenitor cells of various lineages. In addition to its effects on hematopoietic cells, IL-3 can stimulate human endothelial and smooth muscle cell proliferation and motility and can act as a neurotrophic factor with the ability to promote neuronal survival and enhance neurite outgrowth. IL-3-/- mice do appear to exhibit deficiencies in mast cell and basophil development in addition to compromised immunity to parasites and reductions in delayed hypersensitivity reactions.
Charting Kidney Cancer Metabolism Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.Insights into the Function of the Main Class of Drug Targets About thirty percent of all medical drugs such as beta-blockers or antidepressants interact with certain types of cell surface proteins called G protein coupled receptors.Visualizing a Cancer Drug Target at Atomic Resolution Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.Honey’s Potential to Save Lives The healing powers of honey have been known for thousands of years. 3-D Printed Lifelike Liver Tissue for Drug Screening A team led by engineers at the University of California, San Diego has 3D-printed a tissue that closely mimics the human liver's sophisticated structure and function. The new model could be used for patient-specific drug screening and disease modeling. Cytoskeleton Crew Findings confirm sugar's role in helping cancers survive by changing cellular architecture.Biomarker for Recurring HPV-Linked Oropharyngeal Cancers A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University. Valvena, GSK Sign New R&D Collaboration Valneva to supply process development services for EB66® -based Influenza vaccines. Light Signals from Living Cells Fluorescent protein markers delivered under high pressure.Cellular 'Relief Valve' A team led by scientists at The Scripps Research Institute (TSRI) has solved a long-standing mystery in cell biology by showing essentially how a key “relief-valve” in cells does its job.