The MaxDiscovery™ Human Insulin ELISA Test Kit is designed for quantitative determination of the concentration of human insulin in serum, plasma, and cell culture supernatant. Insulin is a hormone with intensive effects on both metabolism and several other body systems (eg, vascular compliance). Insulin causes most of the body's cells to take up glucose from the blood, storing it as glycogen in the liver and muscle, and stops use of fat as an energy source. It is synthesized in the ß-cells of the islets of Langerhans as the precursor, proInsulin, which is processed to form C-peptide and Insulin. The mature Insulin molecule comprises two polypeptide chains, the A chain and B chain. The two chains are linked together by two inter-chain disulphide bridges. Insulin concentrations are severely reduced in Insulin-dependent diabetes mellitus (IDDM) and some other conditions such as hypopituitarism. Insulin levels are raised in non-Insulin-dependent diabetes mellitus (NIDDM), obesity, Insulinoma.