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MaxDiscovery™ Human MMP-3 ELISA Test Kit

Product Description
MaxDiscovery™ Human MMP-3 ELISA Test Kit is designed for quantitative determination of the concentration of human MMP-3 in serum, plasma, and cell culture supernatant. Human matrix metalloproteinase 3 (MMP-3) is also known as stromelysin-1 previously, a protein of 477 amino acid residuals including a short signal peptide of 17 amino acids. MMP-3 may be expressed in fibroblasts, chondrocytes, endothelial cells, macrophages, vascular smooth muscle cells, osteoblasts, and keratinocytes in response to appropriate stimuli. Various agents regulate its biosynthesis. Inflammatory cytokines such as IL-1 and TNF-a, epidermal growth factor, platelet-derived growth factor, phorbol and oncogenic cellular transformation are the inductive agents. MMP-3 belongs to a member of the matrix metalloproteinase (MMP) family which are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP-3 is secreted as an inactive proprotein which is activated when cleaved by extracellular proteinases. MMP-3 appears to play a role in the degradation of fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. MMP-3 is therefore thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22. The method is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes an antibody (capture antibody) specific for Human MMP-3 coated on a 96-well plate. Standards and samples are added to the wells, and any human MMP-3 present binds to the immobilized antibody. The wells are washed and biotinylated polyclonal anti-human MMP-3 antibody (detection antibody) is added. After a second wash, avidin-horseradish peroxidase (avidin-HRP) is added. The wells are again washed and a substrate solution is added, which produces a blue color in direct proportion to the amount of human MMP-3 present in the initial sample. The stop buffer is then added to terminate the reaction. This results in a color change from blue to yellow. The wells are then read at 450 nm.
Product MaxDiscovery™ Human MMP-3 ELISA Test Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 2142-01
Quantity 1 x 96 wells
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BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



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