The MaxDiscovery™ Human TNF-a ELISA Test Kit is designed for quantitative determination of the concentration of human tumor necrosis factor alpha (TNF-a, also known as cachectin) in serum, plasma, and cell culture supernatant. Human TNF-a is a 17.5 kDa polypeptide of 157 amino acids. It is a potent lymphoid factor which exerts cytotoxic effects on a wide range of tumor cells. This cytokine is a primary regulator of inflammatory responses. TNF-a is produced by macrophages, neutrophils, activated T and B lymphocytes, endothelial cells, smooth muscle cells, astrocytes, natural killer cells, lymphokine-activated killer cells, and some transformed cells. TNF-a, and LT-a (lymphotoxin cellular receptors (CD 120a and CD120b), and produce similar effects. TNF-a plays a critical role in the destruction of certain tumors and in the body’s resistance to infection by inducing fever and activating macrophages. Over-production of TNF has been associated with cytotoxic effects such as cachexia. Selected Citations: Shin, S. Y. et al., (April 2011) Total and Interatrial Epicardial Adipose Tissues Are Independently Associated With Left Atrial Remodeling in Patients With Atrial Fibrillation. J Cardiovasc Electr. 22 (6): 647 – 655.
Charting Kidney Cancer Metabolism Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.Insights into the Function of the Main Class of Drug Targets About thirty percent of all medical drugs such as beta-blockers or antidepressants interact with certain types of cell surface proteins called G protein coupled receptors.Visualizing a Cancer Drug Target at Atomic Resolution Using cryo-electron microscopy, researchers were able to view, in atomic detail, the binding of a potential small molecule drug to a key protein in cancer cells.Honey’s Potential to Save Lives The healing powers of honey have been known for thousands of years. 3-D Printed Lifelike Liver Tissue for Drug Screening A team led by engineers at the University of California, San Diego has 3D-printed a tissue that closely mimics the human liver's sophisticated structure and function. The new model could be used for patient-specific drug screening and disease modeling. Cytoskeleton Crew Findings confirm sugar's role in helping cancers survive by changing cellular architecture.Biomarker for Recurring HPV-Linked Oropharyngeal Cancers A look-back analysis of HPV infection antibodies in patients treated for oropharyngeal (mouth and throat) cancers linked to HPV infection suggests at least one of the antibodies could be useful in identifying those at risk for a recurrence of the cancer, say scientists at the Johns Hopkins University. Valvena, GSK Sign New R&D Collaboration Valneva to supply process development services for EB66® -based Influenza vaccines. Light Signals from Living Cells Fluorescent protein markers delivered under high pressure.Cellular 'Relief Valve' A team led by scientists at The Scripps Research Institute (TSRI) has solved a long-standing mystery in cell biology by showing essentially how a key “relief-valve” in cells does its job.