Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>Products>This Product
  Products


MaxDiscovery™ In Cell ELISA Kit

Product Description
The MaxDiscovery™ In Cell ELISA Kit is an enzyme immunoassay for quantitative protein analysis directly in adherent cell cultures—there is no need to lyse cells or run protein gels. The technique can be thought of as an ELISA performed on fixed, permeablized cells rather than on immobilized antigen. Like most ELISA assays, protein is detected using a series of two antibodies, a primary antibody for the protein of interest (not provided with the kit) and an enzyme-conjugated secondary antibody. The protein:antibody complex is visualized by adding an enzyme substrate that, when catalyzed, changes the color of the assay solution within the well of the tissue culture plate so that it can be read in a spectrophotometer at 450 nm. The relative amount of protein in the cells will be directly proportional to the amount of signal that is obtained, making this a simple, convenient, and accurate technique for quantifying specific protein levels within cell lines. The MaxDiscovery™ In Cell ELISA Kit contains materials for washing, fixing, and permeablizing cells in culture. HRP-conjugated anti-mouse and anti-rabbit secondary antibodies are included in the kit so that user-supplied primary antibodies from either mouse or rabbit may be used for detection of the protein of interest. In addition, the kit includes mouse-derived antibody targeting glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is one of the key enzymes involved in glycolysis, and because of its high-level, and constitutive expression, it is widely used as a loading control for Northern/Western blots and for protein normalization. The quantity of GAPDH in a sample is directly proportional to the number of cells in the sample. GAPDH expression is often used as an internal control in gene expression studies. Typically, GAPDH expression is used to normalize expression signal from the gene-of-interest with respect to cell number. This has been particularly useful for RNAi studies or other drug treatment studies. The MaxDiscovery™ In Cell ELISA assay makes it easy to quickly measure levels of GAPDH (positive control) or your protein-of-interest directly in cultured cells for relative quantitative analysis.
Product MaxDiscovery™ In Cell ELISA Kit
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 3440-02
Quantity 2 x 96 wells
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122
Email: info@biooscientific.com



Scientific News
New Biomarker to Assess Stem Cells Developed
A research team led by scientists from UCL have found a way to assess the viability of 'manufactured' stem cells known as induced pluripotent stem cells (iPSCs). The team's discovery offers a new way to fast-track screening methods used in stem cell research.
A Better Model for Parkinson's
Scientists at EPFL solve a longstanding problem with modeling Parkinson’s disease in animals. Using newfound insights, they improve both cell and animal models for the disease, which can propel research and drug development.
Faster Drug Discovery?
Startup develops more cost-effective test for assessing how cells respond to chemicals.
Microalgae Make a Splash in the UK Cosmeceutical Market
Scottish innovators have discovered an anti-viral and anti-inflammatory carbohydrate in microscopic algae (microalgae) which has huge potential to change the cosmetics market.
Mechanism of Tumor Suppressing Gene Uncovered
The most commonly mutated gene in cancer,p53, works to prevent tumor formation by keeping mobile elements in check that otherwise lead to genomic instability, UT Southwestern Medical Center researchers have found.
Experimental Combination Surprises with Anti-HIV Effectiveness
A compound developed to protect the nervous system from HIV surprised researchers by augmenting the effectiveness of an investigational antiretroviral drug beyond anything expected.
Useful Colon Cancer Biomarker Discovered
Biomarker is detectable with simple, inexpensive test.
A New Type of Anticancer Agent
Success in the development of a ?-tubulin specific inhibitor.
Seeing Hope
Gene therapy/drug combo restores some vision in mice with optic nerve injury.
Versatile New Molecule-Building Technique
Chemists at The Scripps Research Institute (TSRI) have devised a new and widely applicable technique for building potential drug molecules and other organic compounds.

SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!