The MaxDiscovery™ Mouse IFN-? ELISA Test Kit is designed for quantitative determination of the concentration of mouse IFN-? in serum, plasma, and cell culture supernatant. Mouse interferon gamma (IFN-?), also called Type II interferon, macrophage activation factor, and immune interferon, is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS polyarylamide gel it appears as a combination of 25, 20 and minor 15.5 kDa bands due to differential glycosylation. IFN-? is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. The biological activity of the IFN-? homodimer is highly species specific and includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, enhances immunoglobulin secretion by B lymphocytes, acts in cytokine regulation and also acts synergistically with other cytokines.