Corporate Banner
Satellite Banner
Biomolecular Screening
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
RNAi Screening Trends
Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
New Tool Uses 'Drug Spillover' to Match Cancer Patients with Treatments
Researchers have developed a new tool that improves the ability to match drugs to disease: the Kinase Addiction Ranker (KAR) predicts what genetics are truly driving the cancer in any population of cells and chooses the best "kinase inhibitor" to silence these dangerous genetic causes of disease.
HIV Susceptibility Linked to Little-Understood Immune Cell Class
High levels of diversity among immune cells called natural killer cells may strongly predispose people to infection by HIV, and may be driven by prior viral exposures, according to a new study.
Sweet Revenge Against Superbugs
A special type of synthetic sugar could be the latest weapon in the fight against superbugs.
Access Denied: Leukemia Thwarted by Cutting Off Link to Environmental Support
A new study reveals a protein’s critical – and previously unknown -- role in the development and progression of acute myeloid leukemia (AML), a fast-growing and extremely difficult-to-treat blood cancer.
Long-sought Discovery Fills in Missing Details of Cell 'Switchboard'
A biomedical breakthrough reveals never-before-seen details of the human body’s cellular switchboard that regulates sensory and hormonal responses.
Tracking Breast Cancer Before it Grows
A team of scientists led by University of Saskatchewan researcher Saroj Kumar is using cutting-edge Canadian Light Source techniques to screen and treat breast cancer at its earliest changes.
Zebrafish Reveal Drugs that may Improve Bone Marrow Transplant
Compounds boost stem cell engraftment; could allow more matches for patients with cancer and blood diseases.
DNA Damage Seen in Patients Undergoing CT Scanning
Along with the burgeoning use of advanced medical imaging tests over the past decade have come rising public health concerns about possible links between low-dose radiation and cancer.
The Light of Fireflies for Medical Diagnostics
EPFL scientists have exploited the light of fireflies in a new method that detects biological molecules without the need for complex devices and high costs.
Scroll Up
Scroll Down

An AlphaScreen® SureFire® Phospho-ERK1/2 Assay Performed on BMG LABTECH’s POLARstar Omega
Bookmark and Share

BMG LABTECH

On activation GPCRs signal through different G-proteins (e.g. Gs, Gi or Gq) to elicit a cellular response which can be mediated through the activation of a number of different signaling cascades which include intracellular Ca2+ mobilization and the formation or inhibition of adenylate cyclase. Although these technologies have been successful in many cases they have had their limitation. Responses for many GPCRs such as Gi coupled GPCRs are not easily measured in current assay formats and other methods have been investigated as alternative readout. One such pathway is the ERK phosphorylation cascade, which results in the phosphorylation of both cytoplasmic and nuclear proteins regulating gene transcription. Hence this type of assay has the ability to measure cellular changes activated by Gs, Gi and Gq coupled receptors giving potential to use a single assay format for screening.

This application note details how the POLARstar Omega is used to detect ERK phosphorylation using a AlphaScreen® SureFire® proximity-based assay. ERK phosphorylation is demonstrated at receptors which signal via Gi and Gq G-proteins.  

Further Information

Related Content

25 Years of Revolutionary Microplate Reader Technology at the LS2
BMG LABTECH exhibits at the biggest Swiss Life Sciences Meeting for the first time.
Tuesday, December 16, 2014
BMG LABTECH Announces First FP User Group Meeting
The meeting brings together a wide range of speakers with extensive experience of Fluorescence Polarization techniques.
Monday, April 26, 2010
BMG LABTECH Announces First FP User Group Meeting
Company announces their inaugural UK Fluorescence Polarisation user group meeting on the 23rd of April 2010.
Monday, April 19, 2010
BMG LABTECH, World Leader in Microplate Reader Technology, is proud to be a Bronze Sponsor of the SBS Conference 2010.
Visit us at booth 817 and join us at our workshops to see latest innovations in microplate reading technology including the ultimate HTS Microplate Reader, PHERAstar FS
Tuesday, March 30, 2010
BMG Labtech Joins ALSSA
The ALSSA - Analytical & Life Science Systems Association - Board of Directors recently approved applications for membership submitted by BMG LABTECH, Inc. Based in Cary, North Carolina.
Thursday, March 25, 2010
DLReady Certification for the Omega Series of Microplate Readers
DLReady certification, awarded by Promega Corp., validates an instrument that has passed the strict performance levels and standards required for the Dual-Luciferase Reporter (DLR) assay system.
Monday, December 03, 2007
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!