Transcriptional and Posttranscriptional Regulation of HIV-1 Gene Expression
The transcription factors that control YAP gene expression are unknown. This study demonstrates that ß-catenin/TCF4 complexes bind a DNA enhancer element within the first intron of the YAP gene to drive YAP expression in CRC cells.

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Cholinesterases, a Target of Pharmacology and Toxicology
This review’s main focus is on cholinesterases as targets of toxins and drugs. The biochemistry of AChE and BuChE is also discussed. Drugs and toxins are divided in chapters according to target sites.

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Launch of DIRECT - An Innovative Medicines Initiative Project for Personalized Medicine in Diabetes
Academia and the pharmaceutical industry working together in the fight against diabetes.

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Estimation of drug-metabolizing capacity by CYP-genotyping and CYP-expression
The lack of therapeutic effect of drugs or the appearance of undesired side-effects is partly caused by differences or changes in drug metabolism. This work aims to introduce an approach of combining CYP-genotyping and CYP-phenotyping tools to estimate patients’ drug-metabolizing capacity.

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Biodegradable Nanoparticles of mPEG-PLGA-PLL Triblock Copolymers as Novel Non-Viral Vectors for Improving siRNA Delivery and Gene Silencing
The gene silencing efficiency of mPEG-PLGA-PLL NPs is shown to be higher than that of commercially available transfecting agent Lipofectamine, while showing no cytotoxicity. The purpose of this work was to synthesize the novel biodegradable mPEG-PLGA-PLL NPs encapsulating siRNA, and examine the physical properties of NPs and in vitro siRNA release profile from NPs.

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Study on Pivot-Point Vibration of Molecular Bond-Rupture Events by Quartz Crystal Microbalance for Biomedical Diagnostics
Molecular bond-rupture scanning is an effective method for the measurement of the energy level necessary to break a bond and distinguishing specific and nonspecific binding of biological molecules.

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FDA Approves Gen-Probe's PROGENSA® PCA3 Assay
Company reveals first molecular test to help determine the need for repeat prostate biopsies in men who have had a previous negative biopsy.

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An ice age flower 30,000 year old revived - resurrection of seed from permafrost
It was an Ice Age squirrel's treasure chamber, a burrow containing fruit and seeds that had been stuck in the Siberian permafrost for over 30,000 years. From the fruit tissues, a team of Russian scientists managed to resurrect an entire plant in a pioneering experiment that paves the way for the revival of other species.

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NIH Common Fund Researchers Uncover Structure of Important Target for Drug Design
Researchers have uncovered a potent class of small molecules that selectively turn on the S1P1 receptor.

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Two Decades of Studying Non-Covalent Biomolecular Assemblies by Means of Electrospray Ionization Mass Spectrometry
MS has evolved to impact a wide range of applications in structural biology. This paper charts some of the milestones in MS development as they pertain to the study of non-covalent complexes, and the novel applications they have enabled.

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OGT Signs Licensing Deal for Colorectal Cancer Biomarkers
OGT has entered into an exclusive licensing agreement with Inven2.

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JHS Secures Multi-Million Dollar Contracts to Manufacture Sterile Parenteral Products
Company has secured contracts with four innovator life science companies.

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Boehringer Ingelheim and Xencor Enter a Collaboration Agreement
Antibodies engineered with Xencor’s proprietary Xtend™ technology for increasing antibody half-life.

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Shire Announces European Approval of Manufacturing Facility for VPRIV®
EMA approval adds significant capacity for the manufacture of Shire’s enzyme replacement therapies.

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Theratechnologies Announces a Supply, Distribution and Licensing Agreement with Actelion
Agreement provides Actelion with the exclusive commercialization rights to tesamorelin in Canada.

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Genticel Completed Recruitment for its Phase Ib Vaccine Trial in HPV Infected Women
Company expects the final results before the end of the second quarter 2012.

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Expression Analysis Announces RNA-SEQ Grant Program
EA teams with Golden Helix and Illumina to offer three fully-funded grants for RNA-Seq studies.

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Debiopharm and Curis Announce Initiation of Phase Ib Expansion Study of Debio 0932
Company expects to initiate a combination Phase I/II study in NSCLC patients in the second quarter of 2012.

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Brenntag Opens New Head Office in India
New office represents Brenntag’s continued commitment to invest in India.

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PRECOS to Unveil New Service Portfolio at Cancer Targets & Therapeutics and AACR 2012
2012 conference examines new and emerging targets and therapeutics in the area of cancer research.

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A Simple, Robust Automated Multiplexed Cell-Based Assay Process for the Assessment of Mitochondrial Dysfunction and Cytotoxicity
Biotek Instruments

Recent studies have shown that an increasing number of drugs no longer on the market have negative effects on mitochondrial function in key organs such as the liver and heart. Therefore it is increasingly important to monitor the effects of lead compounds on mitochondrial function in relevant cell systems. The ability to incorporate a simple, rapid, multiplexed, predictive assay can make the detection of potential toxic effects easier to perform early on in the drug discovery process.

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An Automated, Cell-based Platform for the Rapid Detection of Novel Androgen Receptor Modulators
BioTek Instruments

The Androgen Receptor (AR) is a member of the family of nuclear receptors responsive to steroid hormones. This poster aims to devise, validate and perform a preliminary automated HTS screening campaign to identify novel modulators of AR activity.

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Automated Solutions for Cellular Screening and Characterization of Therapeutic Antibodies for Antibody-Dependent Cellular Cytotoxicity Utility
BioTek Instruments

Since the end of the 1990’s, the pharmaceutical industry has seen an increased interest in biologics, especially in the therapeutic areas of oncology and inflammation. Here we present the automation of two assays for the characterization and selection of potent antibody drug candidates. Both assays rely on HTRF® detection. The first assay quantifies the binding affinity of antibodies to their target antigen, on live cells.

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Validation of an Automated Cell-Based Bioluminescent TNFa Blocker Bioassay
BioTek Instruments

TNFa blocker biopharmaceuticals represent an important and successful class of protein drugs used in the treatment of several autoimmune diseases. Bioassays are indispensible tools in biopharmaceutical drug development and commercialization that are used to quantify biological activity and stability of drugs or drug candidates. The automation of these assays can serve to create an accurate, robust process which can allow the researcher to perform other more important functions.

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Automation of a Generic Fluorescence Methyltransferase Activity Assay
BioTek Instruments

Epigenetic processes are attracting considerable attention in drug discovery as their fundamental roles in controlling normal cell development and contributions to disease states become more clearly defined. This work combines a fluorescence-based assay with liquid handling and dispensing instrumentation and a multi-mode reader which can be used to monitor the biological activity of the histone methyltransferase (HMT) G9a, a model system.

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Automation of a Novel Cell-Based ELISA for Cell Signaling Pathway Analysis
BioTek Instruments

Monitoring and quantifying cell signaling pathways is critical for understanding the behavior of cell processes and many disease states. Monitoring and quantifying cell signaling pathways is critical for understanding the behavior of cell processes and many disease states.

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Efficacy of Using a Combination Microplate Washer for Vacuum-Based DNA Sequencing Reaction Cleanup
BioTek Instruments

The ability to determine the specific pattern of base pairs in DNA molecules is an indispensable part of contemporary molecular biology. This poster demonstrates how the vacuum filtration module available on the BioTek 405 Touch effectively cleans contaminating artifacts from DNA sequencing reactions, which wil contribute to the genomic workflow typical of many molecular biology laboratories and core facilities.

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Improving Cell-Mediated Cytotoxicity Assessment through the Use of an Automated Luminescent ADCC Assay
BioTek Instruments

Assays that can assess the ability of a biosimilar to act in a manner similar to the original biologic have seen increased interest. This poster describes the use of a non-radioactive luminescent chemistry to simplify the assay process and provide improved data quality.

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The Mesenchymal Stem Cells Bioengineered Tissue Using Cobalt-60 Radiation
IPEN/CNEN - USP

The biological effects of gamma radiation by cobalt-60 sources on epidermal-dermal matrix have not been fully elucidated when mesenchymal stem cells cultured add in. This poster shows how scientists standardized the keratinocytes and adipose-derived stem cell (ADSC) culture procedure before constructing a bioengineered dermal-epidermal equivalent populated by keratinocytes and ADSC cultured cells.

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Modular Bench Top Automation
BioTek Instruments

ELISA is one the most utilized assay formats in biomedical research. Numerous clinical, veterinary, and research assays use the specificity of antibodies to identify a diverse array of analytes from any number of different matrices. This poster details a modular bench top workstation capable of automating the assay process steps of most conventional ELISA assays.

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Analyzing Biological Samples
Tecan

This technical note describes the capability of Tecan’s Infinite® 200 PRO multimode reader to regulate the CO2 partial pressure inside its measurement chamber.

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Performance Qualification of an Automated Plate Washer Using the Artel MVS®
Artel

The purpose of this application note is to demonstrate the ability of the MVS® Multichannel Verification System to test the performance of an automated plate washer. The previously written application notes, Using the MVS to Measure Residual Volumes Remaining in a Plate After Sample Aspiration: General Approach1 will be cited within as these unique applications relate to this discussion involving tests for (1) bulk dispense precision and accuracy and (2) volume evacuation efficiency.

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Using the MVS to Measure Residual Volumes Remaining in a Plate After Sample Aspiration: General Approach
Artel

The purpose of this application note is to demonstrate an alternative use for the Artel MVS® Multichannel Verification System. As opposed to measuring the accuracy and precision for a volume transfer of sample into a microtiter plate, the MVS is instead used to measure resididual liquid after sample is aspirated out of each well.

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Artel MVS as a Tool For Measuring Liquid Mixing Efficacy in Microplates
Artel

This application note describes how the ARTEL MVS® Multichannel Verification System can be used as a tool for measuring the efficacy of liquid mixing protocols.

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Evaluation of Forward and Reverse Mode Liquid Handling Techniques Using the ARTEL MVS®
Artel

This study illustrates the use of the Artel MVS® Multichannel Verification System to rapidly evaluate the accuracy and precision of dispensed volumes from the Caliper RapidPlate liquid handler.

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Analysis of Fat-Soluble Vitamins from Food Matrix for Nutrition Labeling
Agilent Technologies

Labelling vitamins in a matrix like infant formula is a mandatory requirement by the FDA. The diversity in the chemical nature of vitamins makes concurrent extraction and analysis of all vitamins challenging. Due to this, analysis of water soluble and fat soluble vitamins will be usually performed separately. This Application Note describes a sen¬sitive and robust method for simulta¬neous determination of nine fat soluble vitamins with UV detection.

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A MIQE Case Study — Effect of RNA Sample Quality and Reference Gene Stability on Gene Expression Data
Bio-Rad Laboratories

In this study, the minichromosome maintenance (MCM) protein, MCM7, was selected as a model target gene to investigate the importance of appropriate reference gene selection and RNA sample quality as described by the MIQE guidelines.

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Analysis of Natural and Artificial Vanilla Preparations
Agilent Technologies

Vanilla extract is widely used as a flavoring ingredient in foods and beverages. It contains approximately 200 substances. Since natural vanilla extract is limited and prices are high, artificial vanilla flavorings are often used. Due to quality and price concerns, it is important to differentiate between these two.

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Tools for Enhancing Sequence Diversity and Reducing Bias in DNA-seq Library Preparation
Bioo Scientific

The generation of high quality next generation sequencing data begins with libraries that have the desired insert size and proper adapter ligation.

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Advanced TC™: A Novel Cell Culture Surface Improving the Cultivation and Differentiation of Embryonic Stem Cells
Greiner Bio-One GmbH

The importance of embryonic stem cells rests in their lack of specialisation. These basic cells are present in the earliest stages of developing embryos and are able to develop into virtually any type of cell and tissue in the body.

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