Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Leiden University uses NanoSight Characterization System to Aid their Drug Delivery Research Programs

Published: Wednesday, February 24, 2010
Last Updated: Wednesday, February 24, 2010
Bookmark and Share
NanoSight helps the research group to focus on vaccine delivery and on protein formulation and characterization with respect to immunogenicity.

The Division of Drug Delivery Technology is part of the Leiden/Amsterdam Center for Drug Research (LACDR), a research institute located at the Gorlaeus laboratories in Leiden in the Netherlands. The group focuses primarily on vaccine delivery and on protein formulation and characterization with respect to immunogenicity. Nanoparticle size and count is vital to these research activities and the Division has selected the NanoSight LM20 to aid their programs.

The leader of the team is Professor Wim Jiskoot. He describes what is important for his research: “The vaccine delivery group aims to develop innovative delivery systems, such as polymeric nanoparticles and liposomes, for the delivery of different types of vaccines through the conventional (injection) or needle-free administration routes (such as transcutaneous or intranasal delivery). It is very important to know the size of the delivery systems as the size can influence the uptake by the cells of the immune system, the diffusion through the skin, the release of vaccine components, and thus the immune response.”

The protein characterization group seeks to understand the causes of unwanted immunogenicity of therapeutic proteins and develop transgenic mouse models capable of predicting immunogenicity of human/humanized proteins in a preclinical setting.

Professor Jiskoot continues: “For the protein group, a good size characterization of protein aggregates is essential to better understand which size class is responsible for triggering unwanted immunogenicity of therapeutic proteins which is believed to be related to the presence of aggregates in the protein formulations. The group aims to stress and thoroughly characterize protein formulations to then test which ones are more immunogenic after their injection in the mouse models.”

Prior to using NanoSight’s LM20 system, the group used a variety of established particle characterization techniques such as Dynamic Light Scattering (DLS), Light Obscuration Particle Counting (LOPC) and Electron Microscopy (EM). However, each has deficiencies in terms of parameters such as sample preparation and speed of use.

The main user is Vasco Filipe and he has seen several benefits which make the NanoSight his system of preference. “We are able to visualize the sample which gives us confidence in our results. Individual particle tracking enables a much better peak resolution than DLS so making it better suited to study polydispersed samples. It gives an approximate particle concentration while letting us see bacterial contamination easily as “swimming” particles.”

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Studying behaviour of Exosomes and Microvesicles using Nanoparticle Tracking Analysis
Dr Gregory Gores of the Mayo Clinic in Rochester, Minnesota is using NTA to study extracellular vesicles.
Tuesday, November 19, 2013
NanoSight Acquired by Malvern Instruments
The acquisition of NanoSight by Malvern Instruments was completed on Friday 27 September.
Monday, September 30, 2013
NanoSight Wins a 2013 Queen's Award for Enterprise – Innovation
Award follows on from their 2012 Queen's Award for Enterprise for International Trade.
Monday, April 22, 2013
Nanoparticle Tracking Analysis Technology Facilitates Cancer Metastasis Research
NanoSight reports on the breakthrough cancer metastasis research of Dr. Hector Peinado Selgas and Dr. David Lyden's research team from Weill Cornell Medical College.
Tuesday, November 13, 2012
Conference Highlights Biomarker Detection Role for NanoSight
Oxford researchers presented new data applying NTA to size and count both cellular microvesicles and exosomes at a low concentration.
Saturday, October 09, 2010
The Forschungszentrum Dresden-Rossendorf Chooses NanoSight to Characterize Magnetic Nanoparticles
NanoSight’s LM-20 nanoparticle characterization system to study magnetic nanoparticles for applications in cancer therapy.
Wednesday, August 18, 2010
NanoSight Holds First European Users Meeting on Nanoparticle Characterization in Langen, Germany
Meeting provided an excellent opportunity to present new data using nanoparticle tracking analysis, NTA.
Thursday, July 15, 2010
NanoSight holds first European users meeting on nanoparticle characterization in Langen, Germany
NanoSight, world-leading manufacturers of unique nanoparticle characterization technology, together with distributor partners Schaefer Technologie, have recently held a meeting of users of their successful range of nanoparticle tracking analysis instruments in Langen, Germany.
Tuesday, July 13, 2010
University of Leicester Uses NanoSight to Characterize Marine Viruses and Bacteriophages
Microbiologists use NanoSight system to study cyanobacteria and their viruses which involves the accurate enumeration of viral particles.
Wednesday, June 09, 2010
Strathclyde’s Nanometrology Centre Selects NanoSight to Develop New Biosensors
University of Strathclyde has selected an LM-10 characterization system to aid in their research and development of new biosensors.
Wednesday, November 11, 2009
NanoSight to Supply Instrumentation for Wellcome Trust Technology Development Grant
NanoSight’s methodologies aimed at measuring cellular nanoparticles in plasma and urine as biomarkers of a broad range of human disease conditions.
Friday, May 22, 2009
NanoSight Nanoparticle Detection Technique Contributes to Virus Production to Counter MRSA
Researchers at the University of Strathclyde use virus particle detection and counting using Nanoparticle Tracking Analysis to combat MRSA.
Tuesday, December 09, 2008
NanoSight Receives £925,000 in Funding Round to Expand their Nanoparticle Characterization Business
The new round of financing which will enable the company to expand the business with the development of new products and sales channels in the US market.
Thursday, November 06, 2008
Nanosight Angel Investor Round
The angel investor round increases its shareholder base and complements previous investments by Venture Capital companies.
Monday, September 10, 2007
NanoSight Raises £250k in Second Tranche of Fundraising
The investment supports Company’s current sales drive and develops capabilities in nanoparticle analysis.
Wednesday, September 27, 2006
Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
Kitchen Utensils Can Spread Bacteria Between Foods
In a recent study researchers found that produce that contained bacteria would contaminate other produce items through the continued use of knives or graters—the bacteria would latch on to the utensils commonly found in consumers' homes and spread to the next item.
Exploring the Causes of Cancer
Queen's research to understand the regulation of a cell surface protein involved in cancer.
Safer, Faster Way To Remove Pollutants From Water
Using nanoparticles filled with enzymes proves more effective than current methods.
Drug May Prevent Life-Threatening Muscle Loss in Advanced Cancers
New data describes how an experimental drug can stop life-threatening muscle wasting (cachexia) associated with advanced cancers and restore muscle health.
Ancient Viral Molecules Essential for Human Development
Genetic material from ancient viral infections is critical to human development, according to researchers at the Stanford University School of Medicine.
Novel Tumor Treatment
In the first published results from a $386,000 National Cancer Institute grant awarded earlier this year, a paper by Scott Verbridge and Rafael Davalos has been published.
Speeding Up the Process of Making Vaccines
System uses a freeze-dry concept to develop "just-add-water" solution.
Chemical Design Made Easier
Rice University scientists prepare elusive organocatalysts for drug and fine chemical synthesis.
New Analysis Technique for Chiral Activity in Molecules
Professor Hyunwoo Kim of the Chemistry Department and his research team have developed a technique that can easily analyze the optical activity of charged compounds by using nuclear magnetic resonance (NMR) spectroscopy.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos