AnaSpec announces the release of a recombinant human BAFF protein. Naïve B-cells, treated with 20-1000 ng/ml BAFF protein prepared by AnaSpec, has been showing increased IgG secretion compared to 2 leading commercial sources.
The sequence (Accession # NP_006564) corresponding to the extracellular domain of human BAFF along with a 6x His tag was expressed in E. coli. Recombinant human BAFF was purified from urea denatured bacterial lysate using immobilized metal affinity chromatography (IMAC) and refolded into its active state using a proprietary technique.
The molecular weight of the recombinant human BAFF is 17.2 kDa. Containing less than 0.1 EU of endotoxin per 1 µg of protein as determined by LAL assay, the activity of BAFF was determined by its ability to stimulate production of immunoglobulin molecules such as IgG, IgA, and IgM in isolated mouse B-lymphocytes. Purified recombinant human BAFF is supplied as sterile and frozen condition at 0.1 mg/ml in 1 x PBS buffer with 5 mM DTT, pH=7.5.