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Simple Method for Measuring Sialic Acids in Infant Formula in Half the Time

Published: Wednesday, December 08, 2010
Last Updated: Wednesday, December 08, 2010
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A new method for the simple determination of sialic acids that are present in infant formula in the pmol range has been announced by Dionex.

The new method is detailed in Application Note 266: Determination of Sialic Acids Using UHPLC with Fluorescence  Detection and demonstrates that N-acetylated sialic acids can be determined and O-acetylated sialic acids can be identified in infant  formula samples using high-performance liquid chromatography (HPLC) with fluorescence detection following acid hydrolysis and  1,2-diamino-4,5-methylenedioxybenzene dihydrochloride (DMB) derivatization. By using a water:acetonitrile gradient, high resolution  of the sialic acids can be obtained in a 20 minute analysis time, compared to the common 40 minute isocratic method.

The described assay uses a short-format Acclaim® RSLC 120 C18 column that allows fast run times. The lower flow rate and shorter  run time requires less acetonitrile than other published methods. The sensitivity of the Dionex FLD-3400RS Fluorescence Detector  easily allows determination of sialic acids in the infant formula that are present in the pmol range. The sensitivity provides for simple  determination of Neu5Ac, Neu5Gc, and O-acetylated sialic acids in the derivatized samples. 


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