Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Virus-induced Gene Silencing in Studying Aphid Resistance in Wheat

Published: Thursday, December 09, 2010
Last Updated: Thursday, December 09, 2010
Bookmark and Share
A research team used barley stripe mosaic virus (BSMV) to target and silence a WRKY53 transcription activator and a phenylalanine-ammonia-lyase (PAL) gene which are both suspected of stimulating aphid defense in wheat.

Virus-induced gene silencing (VIGS) technology is one of the emerging reverse genetic approaches in cereal crops. The use of VIGS in studying aphid defense gene function in wheat has not been explored. Thus, Leon van Eck of Colorado State University, and colleagues, used barley stripe mosaic virus (BSMV) to target and silence a WRKY53 transcription activator and a phenylalanine-ammonia-lyase (PAL) gene which are both suspected of stimulating aphid defense in wheat. They inoculated the resistant wheat with VIGS constructs and observed that the WRKY53 levels decreased, reaching almost the same level as that of the susceptible wheat. At the same time, the PAL expression also decreased, which may imply that both genes are in the same defense response network. Aphid infestation is higher in the silenced plants and there was an increase in the fitness of the aphids compared with those feeding on the control plants. Therefore, it is confirmed that WRKY53 an d PAL  have significant functions in garnering positive resistance against aphids.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
World’s First Therapeutic Venom Database
Open-source library describes nearly 43,000 effects on the human body.
Biologists Induce Flatworms to Grow Heads and Brains of Other Species
Findings shed light on role of a new kind of epigenetic signaling in evolution, could yield clues for understanding birth defects and regeneration.
Fat Cells Originating from Bone Marrow Found in Humans
Cells could contribute to diabetes, heart disease.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos