Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

IBN's 'Fish and Chips' May Help Accelerate Drug Discovery

Published: Monday, April 09, 2012
Last Updated: Monday, April 09, 2012
Bookmark and Share
IBN scientists develop cheaper, faster and more efficient platform for preclinical drug discovery applications.

A cheaper, faster and more efficient platform for preclinical drug discovery applications has been invented by scientists at the Institute of Bioengineering and Nanotechnology (IBN), the world's first bioengineering and nanotechnology research institute.

Called 'Fish and Chips', the novel multi-channel microfluidic perfusion platform can grow and monitor the development of various tissues and organs inside zebrafish embryos for drug toxicity testing.

This research, published recently in Lab on a Chip, has been selected for feature on the journal's back cover (see Image 1 at http://goo.gl/cfqXh).

Zebrafish, and especially their embryos, are an important model for studying diseases and drug screening. The morphological and molecular basis of tissue and organ development in zebrafish embryos resembles that of humans, and the overall drug toxicity is also comparable with that observed in animals.

In contrast to animal models, zebrafish are inexpensive, easily obtainable in large quantity, readily accessible immediately after fertilization, require a shorter development time, and are cheaper to maintain.

Current drug studies on zebrafish embryos are performed on traditional microtiter plates, which do not allow perfusion or the replenishment of growth media and drugs, and cannot facilitate live imaging since the embryos are not fixed in one position due to the size of the well.

The conventional way of visualizing tissues and organs in embryos is a laborious process, which includes first mounting the embryos in a viscous medium such as gel, and then manually orienting the embryos using fine needles.

The embryos also need to be anesthetized to restrict their motion and a drop of saline needs to be continuously applied to prevent the embryos from drying. These additional precautions could further complicate the drug testing results.

The IBN 'Fish and Chips' has been designed for dynamic long-term culturing and live imaging of the zebrafish embryos. The microfluidic platform comprises three parts: 1) a row of eight fish tanks, in which the embryos are placed and covered with an oxygen permeable membrane, 2) a fluidic concentration gradient generator to dispense the growth medium and drugs, and 3) eight output channels for the removal of the waste products (see Image 2 at http://goo.gl/5P6qa).

The novelty of the 'Fish and Chips' lies in its unique diagonal flow architecture, which allows the embryos to be continually submerged in a uniform and consistent flow of growth medium and drugs (see Image 3 at http://goo.gl/VW99M), and the attached gradient generator, which can dispense different concentrations of drugs to eight different embryos at the same time for dose-dependent drug studies.

Other key design elements include customizing the dimensions of the fish tank, which is over 200 times smaller than the individual well of a microtiter plate, to fit the embryos exactly in the tank and restrict its movement for live imaging.

This is crucial to monitor the growth and development of the various tissues and organs in the embryos.

The microfluidic structure was also fabricated using silicon and glass, which provide greater accuracy and reproducibility for commercial application, in comparison with polydimethylsiloxane (PDMS) polymeric-based chips.

Using high-resolution bright-field and fluorescence imaging, the researchers were able to observe the development of various organs such as the eyes, ears, melanophores, brain, yolk sac, trunk and chorion, as well as heartbeats in the zebrafish embryos.

The researchers also conducted drug toxicity testing on the 'Fish and Chips' with valproic acid (VPA), a drug which causes birth defects if consumed by women during their pregnancy.

VPA caused abnormality in the development of the eyes and tail in the embryos. The findings clearly established the proof-of-concept that IBN's 'Fish and Chips' could be used as an organ-level drug screening model.

Professor Hanry Yu, IBN Group Leader, who led the research efforts at IBN, said, "Toxicity is a major cause of drug failures in clinical trials and our novel 'Fish and Chips' device can be used as the first step in drug screening during the preclinical phase to complement existing animal models and improve toxicity testing. The design of our platform can also be modified to accommodate more zebrafish embryos, as well as the embryos of other animal models. Our next step will involve investigating cardiotoxicity and hepatoxicity on the chip."

"Miniaturization is being explored in various ways by our researchers to revolutionize drug development and disease diagnosis. This latest microfluidic platform developed by IBN enables researchers to cut down the time and cost of drug testing significantly. Our technology is available for licensing to companies, and we are also open to collaboration to develop customized assays for drug testing," added Professor Jackie. Y. Ying, IBN Executive Director.

This multi-channel microfluidic perfusion platform was developed in collaboration with Dr Danny van Noort of the MechanoBiology Institute, Singapore, who provided guidance on chip design and Associate Professor Vladimir Korzh's group at the Institute of Molecular and Cell Biology, who provided the transgenic zebrafish lines and fish culture expertise.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More Than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

IBN Creates Unlimited Source of Human Kidney Cells
Applications include in vitro toxicology, disease models & regenerative medicine.
Monday, June 03, 2013
IBN Develops Superior Fuel Cell Material
This technology can be used to power airplanes, vehicles and electronic devices.
Monday, August 27, 2012
Scientific News
The Changing Tides of the In Vitro Diagnostics Market
With the increasing focus in personalized medicine, diagnostics plays a crucial role in patient monitoring.
Immunotherapy Agent Benefits Patients with Drug-Resistant Multiple Myeloma in First Human Trial
Daratumumab proved generally safe in patients, even at the highest doses.
Low-level Arsenic Exposure Before Birth Associated with Early Puberty in Female Mice
Study examine whether low-dose arsenic exposure could have similar health outcomes in humans.
Inciting an Immune Attack On Cancer Cells
A new minimally invasive vaccine that combines cancer cells and immune-enhancing factors could be used clinically to launch a destructive attack on tumors.
‘Mutation-Tracking’ Blood Test for Breast Cancer
Scientists have developed a blood test for breast cancer able to identify which patients will suffer a relapse after treatment, months before tumours are visible on hospital scans.
Cellular Contamination Pathway for Heavy Elements Identified
Berkeley Lab scientists find that an iron-binding protein can transport actinides into cells.
Intensity of Desert Storms May Affect Ocean Phytoplankton
MIT study finds phytoplankton are extremely sensitive to changing levels of desert dust.
Common ‘Heart Attack’ Blood Test May Predict Future Hypertension
Small rises in troponin levels may have value as markers for subclinical heart damage and high blood pressure.
LaVision BioTec Reports on the Neuro Research on the Human Brain After Trauma
Company reports on the work of Dr Ali Ertürk from the Institute for Stroke and Dementia Research at LMU Munich.
NIH Study Shows No Benefit of Omega-3 Supplements for Cognitive Decline
Research was published in the Journal of the American Medical Association.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!