Corporate Banner
Satellite Banner
Scientific Communities
Become a Member | Sign in
Home>News>This Article

Doping Detection Stays a Neck Ahead

Published: Monday, August 13, 2012
Last Updated: Monday, August 13, 2012
Bookmark and Share
Use of LC-MS/MS for the detection of doping in horse racing.

Horse racing is a massive world-wide industry and regular testing is essential to maintain its integrity.

As with human sport, the authorities constantly need to develop methodologies to detect new compounds that drug cheats are using or may start to use. One such compound is peginesatide.

Peginesatide is the first representative of a new class of compounds that mimic the effects of erythropoietin; these include an increase in the number of red blood cells and of haemoglobin levels in the blood.

Both of these increase endurance and so-called erythropoiesis-stimulating agents (ESAs) are banned in human and equine sports.

The approval of the use of peginesatide by the US Food and Drug Administration (FDA) to treat anaemia in patients on dialysis increases its availability and the chance of its use in illegal performance enhancement.

A new mass spectrometry method for detecting peginesatide in humans has already been developed, and now the extension of this for the detection of peginesatide in horse serum is described in an article published in EJMS-European Journal of Mass Spectrometry (doi: 10.1255/ejms.1189) written by Ines Möller, Andreas Thomas, AnkeWingender, Marc Machnik, Wilhelm Schänzer and Mario Thevis from the German Sport University Cologne, Germany.

This is timely since the German Equestrian Federation has recently added peginesatide to its prohibited substance list.

The new method uses electrospray ionization (ESI) liquid chromatography-tandem mass spectrometry (LC-MS/MS). Samples are prepared using the serine endopeptidasesubtilisin to break down the compounds in the sample prior to LC-MS/MS analysis.

According to lead author Mario Thevis, “The method is precise, specific and linear over a wide concentration range. Further, being simple, fast, cost effective, easily transferable to other laboratories and in accordance with the criteria for ‘identification by chromatography and mass spectrometry’ outlined by the Association of Official Racing Chemists (AORC), the method is suitable for routine use in the horse sports drug testing arena.”

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More Than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
High Throughput Mass Spectrometry-Based Screening Assay Trends
Dr John Comley provides an insight into HT MS-based screening with a focus on future user requirements and preferences.
How a Genetic Locus Protects Adult Blood-Forming Stem Cells
Mammalian imprinted Gtl2 protects adult hematopoietic stem cells by restricting metabolic activity in the cells' mitochondria.
Genetic Basis of Fatal Flu Side Effect Discovered
A group of people with fatal H1N1 flu died after their viral infections triggered a deadly hyperinflammatory disorder in susceptible individuals with gene mutations linked to the overactive immune response, according to a recent study.
New Tech Vastly Improves CRISPR/Cas9 Accuracy
A new CRISPR/Cas9 technology developed by scientists at UMass Medical School is precise enough to surgically edit DNA at nearly any genomic location, while avoiding potentially harmful off-target changes typically seen in standard CRISPR gene editing techniques.
The MaxSignal Colistin ELISA Test Kit from Bioo Scientific
Kit can help prevent the antibiotic apocalypse by keeping last resort drugs out of the food supply.
"Good" Mozzie Virus Might Hold Key to Fighting Human Disease
Australian scientists have discovered a new virus carried by one of the country’s most common pest mosquitoes.
Non-Disease Proteins Kill Brain Cells
Scientists at the forefront of cutting-edge research into neurodegenerative diseases such as Alzheimer’s and Parkinson’s have shown that the mere presence of protein aggregates may be as important as their form and identity in inducing cell death in brain tissue.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Potential Treatment for Life-Threatening Viral Infections Revealed
The findings point to new therapies for Dengue, West Nile and Ebola.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos