Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Novel Mass Analyzer Unravels Complex Biomolecular Structures

Published: Tuesday, October 16, 2012
Last Updated: Monday, October 15, 2012
Bookmark and Share
The new mass spectrometer allows the measurement of a range of important proteins and protein assemblies.

In Nature Methods the group of Albert Heck of Utrecht University introduces a new mass spectrometer based on Orbitrap™ technology. This high-sensitive instrument might play a crucial role in the development and use of therapeutic antibodies.

The Heck group, in close collaboration with the research group of Alexander Makarov of Thermo Fisher, the inventor of the Orbitrap analyzer, show that protein assemblies of molecular weights over 1 million Da can be analyzed with very high analytical resolving power and exquisite sensitivity down to detection of single ions.

The new mass spectrometer permits a detailed analytical footprint of biologically and medically important proteins.

In the fast-growing arena of biopharmaceuticals (e.g. therapeutic antibodies) this new instrument will be especially important both in research & development and in quality control, to enable such molecularly complex biomolecules to be used safely in the clinic.

Heck: “The impact of the high mass resolving power at very high sensitivity as achievable with this new mass spectrometer is tremendous; it opens up avenues to measure not only protein-protein interactions, but also covalent and non-covalent binding of small molecules to protein assemblies. Wide-ranging applications may include the direct analysis of drug molecules binding to their targets, and the investigation of post-translational and chemical modifications (e.g. phosphorylation, glycosylation) on intact proteins and protein assemblies. I foresee that this instrument will become instrumental in the development and use of therapeutic antibodies, but also for instance in analyzing how drug molecules such as proteasome inhibitors do interact with their target, the proteasome.”

Makarov: “Presently Orbitrap mass spectrometry is probably the fastest growing mass spectrometric technique. Through this collaboration with Utrecht University we have opened up new avenues for the use of this mass analyzer. I always believed in the versatility of the Orbitrap analyzer, but am still amazed to see that we can now also mass analyze huge protein complexes, even whole viruses, with substantially improved resolving power and mass accuracy and sensitivity down to individual ions.”

This research was made possible by support of the Netherlands Proteomics Centre, and the EU-funded large scale facility PRIME-XS.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More Than 4,600+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Thousands of Protein Interactions Identified
Thanks to the work by Utrecht University researcher Fan Liu and her colleagues, it is now possible to map the interactions between proteins in human cells.
Monday, October 05, 2015
Alexander Makarov Appointed as Professor of HR-MS at Utrecht University
Dr Alexander Makarov is the inventor of the Orbitrap mass analyzer, which changed the field of chemical analysis including proteomics.
Wednesday, November 06, 2013
Scientific News
Platelets are the Pathfinders for Leukocyte Extravasation During Inflammation
Findings from the study could help in the prevention and treatment of inflammatory pathologies.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
How Cancer Spreads in the Body
Cancer cells appear to depend on an unusual survival mechanism to spread around the body, according to an early study led by Queen Mary University of London (QMUL).
Fix for 3-Billion-Year-Old Genetic Error
Researchers at The University of Texas at Austin have developed a fix that allows RNA to accurately proofread for the first time.
“Amazing Protein Diversity” Discovered in Maize
The genome of the corn plant – or maize, as it’s called almost everywhere except the US – “is a lot more exciting” than scientists have previously believed. So says the lead scientist in a new effort to analyze and annotate the depth of the plant’s genetic resources.
Manufactured Stem Cells to Advance Clinical Research
Clinical-grade cell line will enable development of new therapies and accelerate early-stage clinical research.
Dengue Virus Exposure May Amplify Zika Infection
Researchers at Imperial College London have found that the previous exposure to the dengue virus may increase the potency of Zika infection.
Gender Determination in Forensic Investigations
This study investigated the effectiveness of lip print analysis as a tool in gender determination.
Identifying Novel Types of Forensic Markers in Degraded DNA
Scientists have tried to verify the nucleosome protection hypothesis by discovering STRs within nucleosome core regions, using whole genome sequencing.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!