Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Possibilities for Multiphoton Microscopy from Carl Zeiss

Published: Tuesday, October 30, 2012
Last Updated: Tuesday, October 30, 2012
Bookmark and Share
OPO and simultaneous lasers for LSM 7 series.

Multiphoton confocal microscopes from Carl Zeiss now permit the simultaneous use of two NLO lasers or one laser with an optical parametric oscillator (OPO).

Both components are fully integrated and expand the functionality of the multiphoton systems.

In dual laser systems different laser wavelengths simultaneously excite several fluorescent dyes or proteins. Without time loss, users can image specimens with one wavelength and manipulate them in the multiphoton mode with another.

The automatic free beam adjustment gives the system a high degree of stability and reproducibility and ensures exact overlay of the two excitation beams.

Such dual laser systems are used, above all, in intravital microscopy, e.g. for examining functional correlations in the brain of a mouse.

An OPO increases the excitation range of multi- photon microscopy to up to 1300 nanometers and therefore covers in particular the absorption peak of red fluorescent proteins such as mCherry, mPlum and tdTomato.

This efficient, long-wave excitation enables excellent specimen protection. The potentially very high light intensities of the OPO lasers interact with specific structures in the tissue, leading to a doubling and tripling of the oscillation frequency.

This non-linear effect of frequency doubling (SHG) occurs, for example. in striated skeletal muscle and collagen.

Frequency tripling is especially visible on regions where structures with inconsistent optical density converge. These include lipid-water boundaries - for example, between membrane and cytoplasm.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More Than 4,600+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Carl Zeiss Microscopes help to Conserve Britain's Most Fragile Treasure
A stained glass conservation studio has recently embarked upon the conservation of York Minster's Great East Window with help from Carl Zeiss microscopes.
Thursday, January 12, 2012
Scientific News
Platelets are the Pathfinders for Leukocyte Extravasation During Inflammation
Findings from the study could help in the prevention and treatment of inflammatory pathologies.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
Benchtop Automation Trends
Gain a better understanding of current interest in and future deployment of benchtop automated systems.
Manufactured Stem Cells to Advance Clinical Research
Clinical-grade cell line will enable development of new therapies and accelerate early-stage clinical research.
Dengue Virus Exposure May Amplify Zika Infection
Researchers at Imperial College London have found that the previous exposure to the dengue virus may increase the potency of Zika infection.
Gender Determination in Forensic Investigations
This study investigated the effectiveness of lip print analysis as a tool in gender determination.
Identifying Novel Types of Forensic Markers in Degraded DNA
Scientists have tried to verify the nucleosome protection hypothesis by discovering STRs within nucleosome core regions, using whole genome sequencing.
Proteins in Blood of Heart Disease Patients May Predict Adverse Events
Nine-protein test shown superior to conventional assessments of risk.
Higher Frequency of Huntington's Disease Mutations Discovered
University of Aberdeen study shows that the gene change that causes Huntington's disease is much more common than previously thought.
Starving Stem Cells May Enable Scientists To Build Better Blood Vessels
Researchers from the University of Illinois at Chicago College of Medicine have uncovered how changes in metabolism of human embryonic stem cells help coax them to mature into specific cell types — and may improve their function in engineered organs or tissues.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,600+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!