Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Possibilities for Multiphoton Microscopy from Carl Zeiss

Published: Tuesday, October 30, 2012
Last Updated: Tuesday, October 30, 2012
Bookmark and Share
OPO and simultaneous lasers for LSM 7 series.

Multiphoton confocal microscopes from Carl Zeiss now permit the simultaneous use of two NLO lasers or one laser with an optical parametric oscillator (OPO).

Both components are fully integrated and expand the functionality of the multiphoton systems.

In dual laser systems different laser wavelengths simultaneously excite several fluorescent dyes or proteins. Without time loss, users can image specimens with one wavelength and manipulate them in the multiphoton mode with another.

The automatic free beam adjustment gives the system a high degree of stability and reproducibility and ensures exact overlay of the two excitation beams.

Such dual laser systems are used, above all, in intravital microscopy, e.g. for examining functional correlations in the brain of a mouse.

An OPO increases the excitation range of multi- photon microscopy to up to 1300 nanometers and therefore covers in particular the absorption peak of red fluorescent proteins such as mCherry, mPlum and tdTomato.

This efficient, long-wave excitation enables excellent specimen protection. The potentially very high light intensities of the OPO lasers interact with specific structures in the tissue, leading to a doubling and tripling of the oscillation frequency.

This non-linear effect of frequency doubling (SHG) occurs, for example. in striated skeletal muscle and collagen.

Frequency tripling is especially visible on regions where structures with inconsistent optical density converge. These include lipid-water boundaries - for example, between membrane and cytoplasm.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More Than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Carl Zeiss Microscopes help to Conserve Britain's Most Fragile Treasure
A stained glass conservation studio has recently embarked upon the conservation of York Minster's Great East Window with help from Carl Zeiss microscopes.
Thursday, January 12, 2012
Scientific News
Criminal Justice Alcohol Program Linked to Decreased Mortality
Institute has announced that in the criminal justice alcohol program deaths dropped by 4.2 percent over six years.
Charting Kidney Cancer Metabolism
Changes in cell metabolism are increasingly recognized as an important way tumors develop and progress, yet these changes are hard to measure and interpret. A new tool designed by MSK scientists allows users to identify metabolic changes in kidney cancer tumors that may one day be targets for therapy.
Improving Regenerative Medicine
Lab-created stem cells may lack key characteristics, UCLA research finds.
Tick Genome Reveals Secrets of a Successful Bloodsucker
NIH has announced that decipher the genome of the blacklegged tick which could lead to new tick control methods.
"Dark Side" of the Transcriptome
New approach to quantifying gene "read-outs" reveals important variations in protein synthesis and has implications for understanding neurodegenerative diseases.
Individuals' Medical Histories Predicted by their Noncoding Genomes
Researchers have found that analyzing mutations in regions of the genome that control genes can predict medical conditions such as hypertension, narcolepsy and heart problems.
'Molecular Movie' Opens Door to New Cancer Treatments
An international team of scientists led by the University of Liverpool has produced a 'structural movie' revealing the step-by-step creation of an important naturally occurring chemical in the body that plays a role in some cancers.
New Source of Mutations in Cancer
Recently, a new mutation signature found in cancer cells was suspected to have been created by a family of enzymes found in human cells called the APOBEC3 family.
Advancing Synthetic Biology
Living systems rely on a dizzying variety of chemical reactions essential to development and survival. Most of these involve a specialized class of protein molecules — the enzymes.
Madison Researchers Begin Work on Zika Virus
Work will start with basic questions about Zika virus infection.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!