Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Multitasking Plasmonic Nanobubbles Kill some Cells, Modify Others

Published: Thursday, December 06, 2012
Last Updated: Thursday, December 06, 2012
Bookmark and Share
Rice University discovery could simplify and improve difficult processes used to treat diseases, including cancer.

Researchers at Rice University have found a way to kill some diseased cells and treat others in the same sample at the same time. The process activated by a pulse of laser light leaves neighboring healthy cells untouched.

The unique use for tunable plasmonic nanobubbles developed in the Rice lab of Dmitri Lapotko shows promise to replace several difficult processes now used to treat cancer patients, among others, with a fast, simple, multifunctional procedure.

The research is the focus of a paper published online this week by the American Chemical Society journal ACS Nano and was carried out at Rice by Lapotko, research scientist and lead author Ekaterina Lukianova-Hleb and undergraduate student Martin Mutonga, with assistance from the Center for Cell and Gene Therapy at Baylor College of Medicine (BCM), Texas Children’s Hospital and the University of Texas MD Anderson Cancer Center.

Plasmonic nanobubbles that are 10,000 times smaller than a human hair cause tiny explosions. The bubbles form around plasmonicgold nanoparticles that heat up when excited by an outside energy source – in this case, a short laser pulse – and vaporize a thin layer of liquid near the particle’s surface. The vapor bubble quickly expands and collapses. Lapotko and his colleagues had already found that plasmonic nanobubbles kill cancer cells by literally exploding them without damage to healthy neighbors, a process that showed much higher precision and selectivity compared with those mediated by gold nanoparticles alone, he said.

The new project takes that remarkable ability a few steps further. A series of experiments proved a single laser pulse creates large plasmonic nanobubbles around hollow gold nanoshells, and these large nanobubbles selectively destroy unwanted cells. The same laser pulse creates smaller nanobubbles around solid gold nanospheres that punch a tiny, temporary pore in the wall of a cell and create an inbound nanojet that rapidly “injects” drugs or genes into the other cells.

In their experiments, Lapotko and his team placed 60-nanometer-wide hollow nanoshells in model cancer cells and stained them red. In a separate batch, they put 60-nanometer-wide nanospheres into the same type of cells and stained them blue.

After suspending the cells together in a green fluorescent dye, they fired a single wide laser pulse at the combined sample, washed the green stain out and checked the cells under a microscope. The red cells with the hollow nanoshells were blasted apart by large plasmonic nanobubbles. The blue cells were intact, but green-stained liquid from outside had been pulled into the cells where smaller plasmonic nanobubbles around the solid gold nanoparticles temporarily pried open the walls.

Because all of this happens in a fraction of a second, as many as 10 billion cells per minute could be selectively processed in a flow-through system like that under development at Rice, said Lapotko, a faculty fellow in biochemistry and cell biology and in physics and astronomy. That has potential to advance cell and gene therapy and bone marrow transplantation, he said.

Most disease-fighting cell and gene therapies require “ex vivo” – outside the body – processing of human cell grafts to eliminate unwanted (like cancerous) cells and to genetically modify other cells to increase their therapeutic efficiency, Lapotko said. “Current cell processing is often slow, expensive and labor intensive and suffers from high cell losses and poor selectivity. Ideally both elimination and transfection (the introduction of materials into cells) should be highly efficient, selective, fast and safe.”

Plasmonic nanobubble technology promises “a method of doing multiple things to a cell population at the same time,” said Malcolm Brenner, a professor of medicine and of pediatrics at BCM and director of BCM’s Center for Cell and Gene Therapy, who collaborates with the Rice team. “For example, if I want to put something into a stem cell to make it turn into another type of cell, and at the same time kill surrounding cells that have the potential to do harm when they go back into a patient — or into another patient — these very tunable plasmonic nanobubbles have the potential to do that.”

The long-term objective of a collaborative effort among Rice, BCM, Texas Children’s Hospital and MD Anderson is to improve the outcome for patients with diseases whose treatment requires ex vivo cell processing, Lapotko said.

Lapotko plans to build a prototype of the technology with an eye toward testing with human cells in the near future. “We’d like for this to be a universal platform for cell and gene therapy and for stem cell transplantation,” he said.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More Than 4,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

‘Missing Tooth’ Hydrogels Handle Hard-to-Deliver Drugs
Rice University’s custom hydrogel traps water-avoiding molecules for slow delivery.
Wednesday, June 08, 2016
New Cancer Fighters Emerge From Lab
Rice University lab simplifies total synthesis of anti-cancer agent.
Wednesday, May 25, 2016
Cancer Cells Coordinate to Form Roving Clusters
Rice University scientists identify ‘smoking gun’ in metastasis of hybrid cells.
Friday, May 20, 2016
Monovar Drills Down Into Cancer Genome
Rice, MD Anderson develop program to ID mutations in single cancer cells.
Wednesday, May 04, 2016
It’s Now Easier To Go With The Flow
Rice University tool simplifies comparison of flow cytometry data for laboratories.
Wednesday, May 04, 2016
Magnetic Nanoparticles May Reveal Early Traces Of Cancer
Rice University students’ computer program aids MD Anderson diagnostic initiative .
Friday, April 29, 2016
Rare DNA Will Have Nowhere To Hide
Two National Institutes of Health grants back Rice University effort to develop new diagnostics.
Friday, April 08, 2016
Scientists Synthesize Anti-Cancer Agent
A team led by Rice University synthetic organic chemist K.C. Nicolaou has developed a new process for the synthesis of a series of potent anti-cancer agents originally found in bacteria.
Monday, March 14, 2016
‘Big Data’ Drills Down Into Metabolic Details
Rice University bioengineers introduce efficient way to analyze, compare tissue-specific pathways.
Monday, March 14, 2016
Cancer Cells’ Evasive Action Revealed
Rice, MD Anderson scientists analyze suppression of proteins key to immune recognition.
Friday, March 04, 2016
DNA Analysis in the Fast Lane
Rice bioengineers' method should lead to better database of thermal behaviors.
Thursday, January 21, 2016
Bacteria Attack Lignin with Enzymatic Tag Team
Team from Rice, University of Wisconsin-Madison shows how nature handles lignin.
Tuesday, January 12, 2016
Obstacles Not Always a Hindrance to Proteins
Rice researchers’ theory finds blocked path sometimes speeds DNA sequence search.
Friday, December 11, 2015
Red Means ‘Go’ to Therapeutic Viruses
Rice University scientists use light to switch viral activity and deliver cargoes to cells.
Thursday, December 03, 2015
Chemical Design Made Easier
Rice University scientists prepare elusive organocatalysts for drug and fine chemical synthesis.
Wednesday, November 25, 2015
Scientific News
Liquid Biopsies: Miracle Diagnostic or Next New Fad?
Thanks to the development of highly specific gene-amplification and sequencing technologies liquid biopsies access more biomarkers relevant to more cancers than ever before.
Connectome Map More Than Doubles Human Cortex’s Known Regions
Researchers at NIH have developed software that automatically detects the “fingerprint” of each of these areas in an individual’s brain scans.
Discovered Through ‘Big Data’ Analysis
Researchers at the SBP have identified over 100 new genetic regions that affect the immune response to cancer.
Human Stem Cells to Rapidly Generate Bone, Heart Muscle
A new study shows that combining positive and negative signals can quickly and efficiently steer stem cells down complex developmental pathways to become specialized tissues that could be used in the clinic.
New Mechanism of Tuberculosis Infection
Researchers at UTSW Medical Center have identified a new way that tuberculosis bacteria get into the body, revealing a potential therapeutic angle to explore.
New Therapeutic Targets For Small Cell Lung Cancer Identified
Researchers at UTSW Medical Center have identified a protein termed ASCL1 that is essential to the development of small cell lung cancer and that, when deleted in the lungs of mice, prevents the cancer from forming.
Eliminating Doubt in Criminal Investigations
New ASU certificate to help curb error, misunderstanding in the quest for justice.
Determination of 13 Organic Toxicants in Human Blood
Researchers have utilised liquid-liquid extraction coupling HPLC-MS/MS to identify and quantify organic toxicants in human blood.
A Novel Cell Culture Model For Forensic Biology Experiments
Researchers have developed a new cell culture model which provides an efficient research tool in forensic biology.
Rhino DNA Bank Aids Anti-Poaching Fight
At the University of Pretoria's Veterinary Genetics Laboratory (VGL) at Onderstepoort, Dr Cindy Harper and her team have developed a ground-breaking technique to collect and catalogue DNA from rhinos and rhino horns.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!