Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Scientists Discover New Diagnostic Markers for Kawasaki Disease

Published: Friday, December 21, 2012
Last Updated: Thursday, December 20, 2012
Bookmark and Share
Proteins discovered in human urine offers new opportunities for the diagnosis, study and treatment of disease.

Researchers have discovered proteins in human urine that offer new opportunities for the diagnosis, study and maybe even the treatment of Kawasaki disease.

Mass spectrometry-based proteomic analysis of the human urine proteome, the entire set of proteins found in human urine, uncovered molecular markers that offer significant improvements for the diagnosis of the disease.

The results are reported in a new study published in EMBO Molecular Medicine.

“There is no diagnostic test for Kawasaki disease. Currently available diagnostic markers lack the specificity and sensitivity needed for reliable detection of the disease which has motivated our decision to use proteomics to identify new, improved biomarkers,” said Susan Kim, a rheumatologist at Boston Children’s Hospital and an instructor at Harvard Medical School.

Kim continued, “Kawasaki disease is often difficult to diagnose and is the most prevalent cause of acquired childhood heart disease in the developed world. Failure to detect it can lead to coronary artery aneurysms and in some cases death, particularly in children who are not diagnosed early enough or when the diagnosis is not considered and acted upon due to the presence of only some of the classic symptoms.”

Kawasaki disease can occur at any age but is most commonly found in children under the age of five years. The disease appears to influence the immune system in such a way that it attacks its own tissues. This leads to inflammation that can damage blood vessels, most notably around the heart.

If untreated, Kawasaki disease leads to coronary artery aneurysms in up to 25% of cases.

The researchers used highly sensitive mass spectrometry techniques to profile the proteome of urine samples collected from children who had symptoms of Kawasaki disease.

Several molecules were discovered that were exclusively present in the urine of patients with the disease. In particular, elevated levels of filamin C and meprin A were detected in both human blood and urine samples and show considerable potential for use as diagnostics.

Filamin C is a protein that helps maintain the structural integrity of heart and skeletal muscle.

Meprin A is an enzyme that breaks down proteins and which is known to regulate the activities of other proteins linked to inflammation.

Both of these markers could be used to identify patients with Kawasaki disease accurately using tests that are readily amenable for routine medical use.

“The urine proteome consists of thousands of protein molecules. Patients with Kawasaki disease have a unique urinary proteome that is distinct from the proteome observed for children with other causes of fever,” remarked Hanno Steen, director of the Proteomics Center at Boston Children’s Hospital and associate professor at Harvard Medical School.

Steen continued, “In a group of 107 patients, we were able to distinguish children with Kawasaki disease from those with mimicking conditions much more reliably and accurately than currently available testing by measuring their levels of meprin A and filamin C in urine.”

The researchers note that further validation of the diagnostic markers is needed and this work is in progress.

The researchers suggest that the development of clinical tests using these new markers may improve the accuracy of diagnosis of children with suspected Kawasaki disease and assist in the development of new treatments.

For this purpose, the scientists have made the analyzed proteomes openly available at the Peptide Atlas (www.peptideatlas.org).


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,200+ scientific posters on ePosters
  • More Than 4,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Systems-Wide Genetic Investigation Of Blood Pressure Regulation
A genetic investigation of individuals in the Framingham Heart Study may prove useful to identify novel targets for the prevention or treatment of high blood pressure.
Friday, April 17, 2015
Endocrine Disruptors Impair Human Sperm Function
Endocrine disruptors may contribute to widespread fertility problems.
Tuesday, May 13, 2014
Small Non-coding RNAs Could be Warning Signs of Cancer
Results are published in EMBO reports.
Tuesday, February 18, 2014
Alternative Allocation of Science Funding Proposed
The method depends on a collective distribution of funding by the scientific community.
Wednesday, January 08, 2014
Pancreatic Stem Cells Isolated from Mice
Scientists have succeeded in growing stem cells that have the ability to develop into two different types of cells that make up a healthy pancreas.
Tuesday, September 17, 2013
New Muscular Dystrophy Treatment Shows Promise in Early Study
A preclinical study has found that a new oral drug shows early promise for the treatment of muscular dystrophy.
Monday, September 09, 2013
Alzheimer’s Disease Protein Controls Movement in Mice
A protein well known for its role in Alzheimer’s disease controls spindle development in muscle and leads to impaired movement in mice when the protein is absent or treated with inhibitors.
Monday, June 24, 2013
How Disease Mutations Affect the Parkin Protein
Researchers at the MRC Laboratory of Molecular Biology have determined the crystal structure of Parkin, a protein found in cells that when mutated can lead to a hereditary form of Parkinson’s disease.
Monday, June 03, 2013
EMBO Announces 52 New Members for 2013
EMBO announced today that 52 outstanding researchers in the life sciences were newly elected to its membership.
Tuesday, May 21, 2013
Thijn Brummelkamp Receives the EMBO Gold Medal for 2013
The award acknowledges his outstanding work to accelerate the genetic analysis of human disease.
Wednesday, May 08, 2013
Cancer Stem Cells Isolated from Kidney Tumours
Scientists have isolated cancer stem cells that lead to the growth of Wilms’ tumours, a type of cancer typically found in the kidneys of young children.
Friday, December 14, 2012
EMBO, EMBC and the National Science Council of Taiwan Sign Cooperation Agreement
New ways of global scientific interaction have been created following a cooperation agreement between EMBO, the European Molecular Biology Conference (EMBC), and the National Science Council of Taiwan (NSC).
Thursday, November 29, 2012
Brain Neurons and Diet Influence Onset of Obesity and Diabetes in Mice
Mice lacking AgRP-neurons adapt poorly to a carbohydrate diet and their metabolism seems better suited for feeding on fat.
Friday, September 21, 2012
Danes, the wisest among Europeans
A survey of Danish citizens support using GM plants for production of pharmaceuticals
Friday, October 02, 2009
Scientific News
Open Source Seed Initiative – A Welcome Boost to Global Crop Breeding
A team of plant breeders, farmers, non-profit agencies, seed advocates, and policymakers have created the Open Source Seed Initiative.
ASMS 2016: Targeting Mass Spectrometry Tools for the Masses
The expanding application range of MS in life sciences, food, energy, and health sciences research was highlighted at this year's ASMS meeting in San Antonio, Texas.
A New Way Out for Stem Cells
Researchers at North Carolina State University have discovered that therapeutic stem cells exit the bloodstream in a different manner than was previously thought.
One Giant Leap for the Future of Safe Drug Delivery
Sheffield engineers make major breakthrough in developing silk ‘micro-rockets’ that can be used safely in biological environments.
Designing Potential AIDS Vaccine Candidates
Findings represent ‘big accomplishment’ in biomedical engineering and design.
Anticancer Drug Stops Ebola Virus Molecule in its Tracks
A team of scientists from the University of Oxford have successfully mapped the structure of the Ebola virus molecule that drives the attack strategy and leads to fatal infections in humans.
Assessing the Effectiveness of Genome-Editing Technologies
Researchers have developed a cost-effective and rapid method for assessing edits generated by CRISPR-Cas9 and other genome-editing technologies.
Anthrax Proteins Might Help Treat Cancerous Tumors
Studies in mice reveal novel treatment regimen.
New Cancer Drug Target Found in Dual-Function Protein
Findings from a study from TSRI have shown that targeting a protein called GlyRS might help to halt cancer growth.
Key to Chronic Fatigue Syndrome is in Your Gut, Not Head
Researchers report they have identified biological markers of the disease in gut bacteria and inflammatory microbial agents in the blood.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,200+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!