Corporate Banner
Satellite Banner
Technology
Networks
Scientific Communities
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Maglev Tissues Could Speed Toxicity Tests

Published: Monday, January 28, 2013
Last Updated: Monday, January 28, 2013
Bookmark and Share
Scientists use magnetic levitation to make in vitro lung tissue more realistic.

In a development that could lead to faster and more effective toxicity tests for airborne chemicals, scientists from Rice University and the Rice spinoff company Nano3D Biosciences have used magnetic levitation to grow some of the most realistic lung tissue ever produced in a laboratory.

The research is part of an international trend in biomedical engineering to create laboratory techniques for growing tissues that are virtually identical to those found in people’s bodies. In the new study, researchers combined four types of cells to replicate tissue from the wall of the bronchiole deep inside the lung.

The research is available online and scheduled to appear in a future issue of the journal Tissue Engineering Part C: Methods.

“One of the unique things about the magnetic levitation technology is that it allows us to move cells around and arrange them the way that we want for a particular types of tissue,” said study co-author Tom Killian, professor and department chair of physics and astronomy at Rice. “This is the first time anyone has arranged these four cell types in the same way that they are found in lung tissue.”

In vitro laboratory tests have historically been conducted on 2-D cell cultures grown in flat petri dishes, but scientists have become increasingly aware that cells in flat cultures sometimes behave and interact differently than cells that are immersed in 3-D tissue.

Killian and fellow scientists from Rice and the University of Texas MD Anderson Cancer Center co-founded Nano3D Biosciences in 2009 after creating a technology that uses magnetism to levitate and grow 3-D cell cultures. The technology relies on inert, nontoxic magnetic nanoparticles that are inserted into the living cells. Researchers can then use magnets to lift and suspend the cells as they grow and divide.

“Growing realistic lung tissues in vitro is a particular challenge,” said study co-author Jane Grande-Allen, professor of bioengineering at Rice. “There are a number of technical obstacles, and scientific funding agencies have placed a particular emphasis on lung tissue because there’s a large potential payoff in terms of reducing costs for pharmaceutical and toxicological testing.”

Nano3D Biosciences won a Small Business Innovation Research (SBIR) grant from the National Science Foundation (NSF) in 2011 to create a four-layered lung tissue from endothelial cells, smooth muscle cells, fibroblasts and epithelial cells.

Glauco Souza, the company’s chief scientific officer and co-founder, said the project switched into high gear when Rice bioengineering graduate student Hubert Tseng joined the research team as an intern. Tseng was already a student in Grande-Allen’s lab, one of Rice’s leading laboratories for tissue-engineering research.

“Hubert’s and Jane’s expertise in tissue engineering was invaluable for tackling this problem,” Souza said.

Another collaboration that paid off big was a partnership with a group of undergraduate students at Rice’s Oshman Engineering Design Kitchen. The undergraduate team, Cells in 3-D, worked on a magnetic pen that could be used to grab, move and combine magnetized 3-D cell cultures. Souza said Tseng used a version of this tool to create layered bronchiole tissues for this new study.

Tseng said the new tissue resembles native bronchiole tissue more closely than any other tissue yet created in the lab.

“We conducted a number of tests, and the tissue has the same biochemical signature as native tissue,” Tseng said. “We also used primary cells rather than engineered cells, which is important for toxicological testing because primary cells provide the closest possible match to native cells.”

Souza said bronchiole tissue could solve another problem that’s frequently encountered in testing the toxicity of airborne agents.

“With traditional 2-D cultures, it is very difficult to culture cells at the air-liquid interface, which is what you’d prefer for toxicity testing,” he said. “With our technology, we can easily levitate the bronchiole tissue to the air-liquid interface so that airborne toxins are exposed to the epithelial layer of the tissue, just as it would occur in the lungs.”

Grande-Allen said Tseng and other members of her group have already used the same methods pioneered in the bronchiole study to produce heart valve tissue; Souza said the NSF has awarded the company with a second phase of SBIR funding to further develop the technique for other types of tissue.

Study co-authors include Robert Raphael, professor of bioengineering at Rice and co-founder of Nano3D Biosciences; Dr. Robert Moore, a pediatric pulmonologist at Baylor College of Medicine (BCM); and former BCM scientist Jacob Gage, now with Nano3D Biosciences.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More Than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

DNA Analysis in the Fast Lane
Rice bioengineers' method should lead to better database of thermal behaviors.
Thursday, January 21, 2016
Bacteria Attack Lignin with Enzymatic Tag Team
Team from Rice, University of Wisconsin-Madison shows how nature handles lignin.
Tuesday, January 12, 2016
Obstacles Not Always a Hindrance to Proteins
Rice researchers’ theory finds blocked path sometimes speeds DNA sequence search.
Friday, December 11, 2015
Red Means ‘Go’ to Therapeutic Viruses
Rice University scientists use light to switch viral activity and deliver cargoes to cells.
Thursday, December 03, 2015
Chemical Design Made Easier
Rice University scientists prepare elusive organocatalysts for drug and fine chemical synthesis.
Wednesday, November 25, 2015
Biomarker Finder Adjusts On the Fly
Rice University scientists build better tool to find signs of disease.
Thursday, October 22, 2015
Gene On-Off Switch Works Like Backpack Strap
Texas Medical Center-based team unravels how loops form in genome.
Thursday, October 22, 2015
Structure of Protein at Root of Muscular Disease Decoded
Researchers at Rice University and Baylor College of Medicine have unlocked the structural details of a protein seen as key to treating a neuromuscular disease.
Thursday, October 01, 2015
Researchers Find New Clue to Halting Leukemia Relapse
A protein domain once considered of little importance may be key to helping patients who are fighting acute myeloid leukemia (AML) avoid a relapse.
Friday, September 11, 2015
Imaging Software Could Speed Breast Cancer Diagnosis
Technology could improve access to diagnostic services in developing countries.
Wednesday, August 26, 2015
Researchers Strategize to Outsmart Bacteria
Rice University lab identifies mutations that allow bacteria to resist antibiotics.
Thursday, August 06, 2015
Cancer Treatment Models get Real
Researchers at Rice Univ. and Univ. of Texas MD Anderson Cancer Center have developed a way to mimic the conditions under which cancer tumors grow in bones.
Thursday, August 06, 2015
Bacteria Use DNA Replication to Time Key Decision
Rice University researchers have found that in spore-forming bacteria, chromosomal locations of genes can couple the DNA replication cycle to critical decisions about whether to reproduce or form spores.
Monday, July 13, 2015
Massive Genome Shift in one Generation
A team of biologists has discovered that an agricultural pest that began plaguing U.S. apple growers in the 1850s likely did so after undergoing extensive and genome-wide changes in a single generation.
Tuesday, June 16, 2015
DNA Mutations get Harder to Hide
Rice University researchers have developed a method to detect rare DNA mutations with an approach hundreds of times more powerful than current methods.
Wednesday, May 27, 2015
Scientific News
Natural Protein Points to New Inflammation Treatment
Findings may offer insight to effective treatments for inflammatory diseases, such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
Genetic Cause of Rare Allergy
Institute has identified a genetic mutation responsible for a rare form of inherited hives induced by vibratory urticaria.
Battery Component Found to Harm Key Soil Microorganism
The material at the heart of the lithium ion batteries that power electric vehicles, laptop computers and smartphones has been shown to impair a key soil bacterium, according to new research.
Keeping Tumor Growth at Bay
Engineers at Washington University in St. Louis found a way to keep a cancerous tumor from growing by using nanoparticles of the main ingredient in common antacid tablets.
Natural Protein Points to New Inflammation Treatment
Findings may offer insight to effective treatments for inflammatory diseases, such as rheumatoid arthritis, psoriasis, and multiple sclerosis.
Mitochondria Shown to Trigger Cell Ageing
An international team of scientists has for the first time shown that mitochondria, the batteries of the cells, are essential for ageing.
Cancer Cells Kill Off Healthy Neighbours
Cancer cells create space to grow by killing off surrounding healthy cells, according to UK researchers working with fruit flies.
Validating the Accuracy of CRISPR-Cas9
IBS Researchers create multiplex Digenome-seq to find errors in CRISPR-Cas9 processes.
Cancer Drug Target Visualized at Atomic Resolution
New study using cryo-electron microscopy shows how potential drugs could inhibit cancer.
Genetic Mechanism Behind Cancer-Causing Mutations
Researchers at Indiana University has identified a genetic mechanism that is likely to drive mutations that can lead to cancer.
Scroll Up
Scroll Down
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!